An In Vitro Absorbance Assay for Skin Cell Viability and Function




The Easy and Rapid In Vitro Research Absorbance Assay for Skin Cells

Cell Population Species Medium Formulation Catalog Number Quantity
Skin Cells (adherent) Any User defined KDL-A-1 1 Kit
Skin Cells (non-adherent) Any User defined KDL-NA-1 1 Kit

SkinLight™: An In Vitro Absorbance Assay for Skin Cell Viability and Function


  • Study epidermal stem cell function, self-renewal and proliferation.
  • Factors affecting wound healing.
  • Determine the metabolic viability of different cell types derived from skin tissue.
  • The role of adhesion factors.
  • Study the effect of agents on skin function in different cell types.
  • Study the effect of nanoparticles on skin cell types.
  • Study diseases of the skin.
  • Incorporates the non-destructive and proven Roche® WST-1 tetrazolium reagent to produce a soluble yellow formazan product.  
  • Uses an absorbance plate reader or multimode plate reader, with a 420nm to 480nm filter.
  • Non-subjective, instrument-based and quantitative readout procedure.
  • Combine directly with other endpoint assays.
  • Flexibility to use investigators own growth medium and culture protocols.
  • Available for adherent or non-adherent cells.
  • High-throughput capability.
  • Results in 1-4 hours after addition of the single-step WST-1 reagent.
  • Simple, time efficient and cost effective.

Principle of Using STEMLight Assays on Multiple Proliferating Cell Types Using an Absorbance / Colorimetric Readout
SkinGlo™ can be used for primary skin cells as well as skin cell lines from multiple species.
  • Incorporates a terazolium WST-1 reagent that is reduced to a soluble yellow formazan, which can be measured at an maximum absorbance of 440nm (420nm to 480nm) in an absorbance plate reader.
  • After culture, add 10μl of the WST-1 reagent, mix and read absorbance at 440nm after 1-4 hours in a 96-well plate reader. Plates can be returned to incubator and re-read at a later time for increased sensitivity.
  • No solubilization step as in a MTT reaction. Replaces MTT assays.

For Research Use Only. Not for clinical diagnostic use.
Absorbance or multimode plate reader with a 490nm filter.

  • WST-1 Reagent.
  • Sterile, clear, 96-well plates. Choice of non-adherent or adherent plates.
  • Non-sterile, 96-well plate for background determination.
  • Sterile, adhesive foil covers to maintain sterility of unused wells.
PLEASE NOTE. The investigator has the flexibility to culture skin cells using their own growth medium.