An In Vitro Absorbance Assay for Skin Cell Viability and Function
The Easy and Rapid In Vitro Research Absorbance Assay for Skin Cells
|Cell Population||Species||Medium Formulation||Catalog Number||Quantity|
|Skin Cells (adherent)||Any||User defined||KDL-A-1||1 Kit|
|Skin Cells (non-adherent)||Any||User defined||KDL-NA-1||1 Kit|
Uses of SkinLight™
- Study epidermal stem cell function, self-renewal and proliferation.
- Factors affecting wound healing.
- Determine the metabolic viability of different cell types derived from skin tissue.
- The role of adhesion factors.
- Study the effect of agents on skin function in different cell types.
- Study the effect of nanoparticles on skin cell types.
- Study diseases of the skin.
- Incorporates the non-destructive and proven Roche® WST-1 tetrazolium reagent to produce a soluble yellow formazan product.
- Uses an absorbance plate reader or multimode plate reader, with a 420nm to 480nm filter.
- Non-subjective, instrument-based and quantitative readout procedure.
- Combine directly with other endpoint assays.
- Flexibility to use investigators own growth medium and culture protocols.
- Available for adherent or non-adherent cells.
- High-throughput capability.
- Results in 1-4 hours after addition of the single-step WST-1 reagent.
- Simple, time efficient and cost effective.
SkinGlo™ can be used for primary skin cells as well as skin cell lines from multiple species.SkinLight™ Principle
- Incorporates a terazolium WST-1 reagent that is reduced to a soluble yellow formazan, which can be measured at an maximum absorbance of 440nm (420nm to 480nm) in an absorbance plate reader.
- After culture, add 10Î¼l of the WST-1 reagent, mix and read absorbance at 440nm after 1-4 hours in a 96-well plate reader. Plates can be returned to incubator and re-read at a later time for increased sensitivity.
- No solubilization step as in a MTT reaction. Replaces MTT assays.
For Research Use Only. Not for clinical diagnostic use.
Absorbance or multimode plate reader with a 490nm filter.
- WST-1 Reagent.
- Sterile, clear, 96-well plates. Choice of non-adherent or adherent plates.
- Non-sterile, 96-well plate for background determination.
- Sterile, adhesive foil covers to maintain sterility of unused wells.