HepatoLight™

An In Vitro Absorbance Assay for Hepatocyte Viability and Function


 

 

HepatoLight™

The Easy and Rapid In Vitro Research Absorbance Assay for Hepatocytes

Cell Population Species Medium Formulation Catalog Number Quantity
Hepatocytes (adherent) Any User defined KHL-A-1 1 Kit
Hepatocytes (non-adherent) Any User defined KHL-NA-1 1 Kit

HepatoLight™: An In Vitro Absorbance Assay for Hepatocyte Viability and Function

 

  • Determine viability of hepatocyte cultures.
  • Measure hepatocyte life span in culture.
  • Study hepatocyte stem cells.
  • Determine hepatocyte mitochondrial dysfunction.
  • Study liver cell interactions.
  • Determine the response of hepatocytes to different agents.
  • Incorporates the non-destructive and proven Roche® WST-1 tetrazolium reagent to produce a soluble yellow formazan product.  
  • Uses an absorbance plate reader or multimode plate reader, with a 420nm to 480nm filter.
  • Non-subjective, instrument-based and quantitative readout procedure.
  • Combine directly with other endpoint assays.
  • Flexibility to use investigators own growth medium and culture protocols.
  • Available for adherent or non-adherent cells.
  • High-throughput capability.
  • Results in 1-4 hours after addition of the single-step WST-1 reagent.
  • Simple, time efficient and cost effective.

Principle of Using STEMLight Assays on Multiple Proliferating Cell Types Using an Absorbance / Colorimetric Readout
 

HepatoLight™ can be used for multiple species and different sources of hepatocytes (e.g. fresh, cryopreserved, male, female, mixed and hepatocyte cell lines such as HepG2).
  • Incorporates a terazolium WST-1 reagent that is reduced to a soluble yellow formazan, which can be measured at an maximum absorbance of 440nm (420nm to 480nm) in an absorbance plate reader.
  • After culture, add 10μl of the WST-1 reagent, mix and read absorbance at 440nm after 1-4 hours in a 96-well plate reader. Plates can be returned to incubator and re-read at a later time for increased sensitivity.
  • No solubilization step as in a MTT reaction. Replaces MTT assays.

For Research Use Only. Not for clinical diagnostic use.
Absorbance or multimode plate reader with a 490nm filter.

  • WST-1 Reagent.
  • Sterile, clear, 96-well plates.
  • Non-sterile, 96-well plate for background determination.
  • Sterile, adhesive foil covers to maintain sterility of unused wells.
PLEASE NOTE. The investigator has the flexibility to culture hepatocytes using their own hepatocyte growth and/or maintenance medium. However, HepatoGro™ medium can be purchased separately from Preferred Cell Systems™.
Please visit the Instructional Videos page
 
Download the HepatoLight™ Technical Manual
Download Certificate of Analysis for Sterile 96-Well Plates
Download Certificate of Analysis for Non-Sterile, 96-Well Plates
Download Certificate of Analysis for Sealing Films