An In Vitro Absorbance Assay for Cardiomyocyte Viability and Function




The Easy and Rapid In Vitro Research Absorbance Assay for Cardiomyocytes

Cell Population Species Medium Formulation Catalog Number Quantity
Cardiomyocytes (adherent) Any User defined KCL-A-1 1 Kit
Cardiomyocytes (non-adherent) Any User defined KCL-NA-1 1 Kit

CardioLight™: An In Vitro Absorbance Assay for Cardiomyocyte Viability and Function


  • Determine viability of cultured myocytes.
  • Measure cardiomyocyte life span.
  • Study the role of adhesion molecules and ions on cardiomyocyte function.
  • Determine cardiomyocyte mitochondrial dysfunction.
  • Study the effect of agents on the contractile apparatus and excitation and contraction coupling.
  • Study mechanisms of injury.
  • Do cardiac stem cells exist? Examine stem cell function as they relate to cardiac cells.
  • Incorporates the non-destructive and proven Roche® WST-1 tetrazolium reagent to produce a soluble yellow formazan product.  
  • Uses an absorbance plate reader or multimode plate reader, with a 420nm to 480nm filter.
  • Non-subjective, instrument-based and quantitative readout procedure.
  • Combine directly with other endpoint assays.
  • Flexibility to use investigators own growth medium and culture protocols.
  • Available for adherent or non-adherent cells.
  • High-throughput capability.
  • Results in 1-4 hours after addition of the single-step WST-1 reagent.
  • Simple, time efficient and cost effective.

Principle of Using STEMLight Assays on Multiple Proliferating Cell Types Using an Absorbance / Colorimetric Readout

CardioLIGHT™ can be used for multiple species.
  • Incorporates a terazolium WST-1 reagent that is reduced to a soluble yellow formazan, which can be measured at an maximum absorbance of 440nm (420nm to 480nm) in an absorbance plate reader.
  • After culture, add 10μl of the WST-1 reagent, mix and read absorbance at 440nm after 1-4 hours in a 96-well plate reader. Plates can be returned to incubator and re-read at a later time for increased sensitivity.
  • No solubilization step as in a MTT reaction. Replaces MTT assays.

For Research Use Only. Not for clinical diagnostic use.
Absorbance or multimode plate reader with a 490nm filter.

  • WST-1 Reagent.
  • Sterile, clear, 96-well plates. Choice of non-adherent or adherent plates.
  • Non-sterile, 96-well plate for background determination.
  • Sterile, adhesive foil covers to maintain sterility of unused wells.
PLEASE NOTE. The investigator has the flexibility to culture cardiac myocytes using their own growth medium and culture protocols.