An In Vitro Absorbance Assay for Intestinal Cell Viability and Function



The Easy and Rapid In Vitro Research Absorbance Assay for Intestinal Cells

Cell Population Species Medium Formulation Catalog Number Quantity
Intestinal Cells (adherent) Any User defined KEL-A-1 1 Kit
Intestinal Cells (non-adherent) Any User defined KEL-NA-1 1 Kit

EpiLight™: An In Vitro Absorbance Assay for Tumor Cell Viability and Function


  • Study intestinal epithelium stem cell research.
  • Measure stem cell self-renewal, proliferation potential and ability.
  • Study intestinal progenitor cells, cell differentiation and markers.
  • Study cell functional interaction.
  • Determine the response of crypt and villi cells to different agents.
  • Incorporates the non-destructive and proven Roche® WST-1 tetrazolium reagent to produce a soluble yellow formazan product.  
  • Uses an absorbance plate reader or multimode plate reader, with a 420nm to 480nm filter.
  • Non-subjective, instrument-based and quantitative readout procedure.
  • Combine directly with other endpoint assays.
  • Flexibility to use investigators own growth medium and culture protocols.
  • Available for adherent or non-adherent cells.
  • High-throughput capability.
  • Results in 1-4 hours after addition of the single-step WST-1 reagent.
  • Simple, time efficient and cost effective.

Principle of Using STEMLight Assays on Multiple Proliferating Cell Types Using an Absorbance / Colorimetric Readout

EpiLIGHT™ can be used for to study intestinal cells from multiple species. Intestinal cell lines can also be used with EpiLIGHT™
  • Incorporates a terazolium WST-1 reagent that is reduced to a soluble yellow formazan, which can be measured at an maximum absorbance of 440nm (420nm to 480nm) in an absorbance plate reader.
  • After culture, add 10μl of the WST-1 reagent, mix and read absorbance at 440nm after 1-4 hours in a 96-well plate reader. Plates can be returned to incubator and re-read at a later time for increased sensitivity.
  • No solubilization step as in a MTT reaction. Replaces MTT assays.

For Research Use Only. Not for clinical diagnostic use.
Absorbance or multimode plate reader with a 490nm filter.

  • WST-1 Reagent
  • Sterile, clear, 96-well plates. Choice of non-adherent or adherent plates.
  • Non-sterile, 96-well plate for background determination.
  • Sterile, adhesive foil covers to maintain sterility of unused wells.
EpiLIGHT™ has the flexibility to use virtually any growth medium and culture protocol.