HALO®-Tox HT
A Standardized and Validated In Vitro
ATP Bioluminescence Lympho-Hematotoxicity /
Bone Marrow Toxicity Testing and
High-Throughput Screening Platform
Buy HALO-Tox HT™ for Human Cells
HALO-Tox HT™
A Fast and Easy "Global" Fluorescence Assay to Monitor Lympho-Hematopoietic Reconstitution After Transplantation
| Cell Population | Species | Formulation | Catalog Number | Quantity | |
|---|---|---|---|---|---|
| Stem Cells | |||||
| SC-HPP 2 | Human | Low serum | K2-HPP2-4HH | 4-Plate /1 Kit | |
| SC-GEMM 1 | Human | Low serum | K2-GEMM1-4HH | 4-Plate /1 Kit | |
| Stem Cells | |||||
| SC-HPP 2 | Human | Serum-free | K2SF-HPP2-4HH | 4-Plate /1 Kit | |
| SC-GEMM 1 | Human | Serum-free | K2SF-GEMM1-4HH | 4-Plate /1 Kit | |
| Hematopoietic Progenitor Cells | |||||
| P-BFU 1 | Human | Low serum | K2-BFU1-4HH | 4-Plate /1 Kit | |
| P-GM 1 | Human | Low serum | K2-GM1-4HH | 4-Plate /1 Kit | |
| P-Mk 1 | Human | Low serum | K2-Mk1-4HH | 4-Plate /1 Kit | |
| Hematopoietic Progenitor Cells | |||||
| P-BFU 1 | Human | Serum-free | K2SF-BFU1-4HH | 4-Plate /1 Kit | |
| P-GM 1 | Human | Serum-free | K2SF-GM1-4HH | 4-Plate /1 Kit | |
| P-Mk 1 | Human | Serum-free | K2SF-Mk1-4HH | 4-Plate /1 Kit | |
| Lymphopoietic Progenitor Cells | |||||
| P-Tcell 3 | Human | Low serum | K2-T3-4HH | 4-Plate /1 Kit | |
| P-Bcell 3 | Human | Low serum | K2-B3-4HH | 4-Plate /1 Kit | |
| Lymphopoietic Progenitor Cells | |||||
| P-Tcell 3 | Human | Low serum | K2-T3-4HH | 4-Plate /1 Kit | |
| P-Bcell 3 | Human | Low serum | K2-B3-4HH | 4-Plate /1 Kit | |
| No Growth Factors Added | |||||
| Any | Human | Low serum | K2-0-4HH | 4-Plate /1 Kit | |
| Any | Human | Serum-free | K2SF-0-4HH | 4-Plate /1 Kit | |
| "Global" Research Assays | |||||
| "Global" 4-Pop Tox | Human | Low serum | K2-4P-5HH | 5-Plate/1 Kit | |
| "Global" 5-Pop Tox | Human | Low serum | K2-5P-6HH | 6-Plate/1 Kit | |
| "Global" 7-Pop Tox | Human | Low serum | K2-7P-8HH | 8-Plate/1 Kit | |
| "Global" Research Assays | |||||
| "Global" 4-Pop Tox | Human | Serum-free | K2SF-4P-5HH | 5-Plate/1 Kit | |
| "Global" 5-Pop Tox | Human | Serum-free | K2SF-5P-6HH | 6-Plate/1 Kit | |
| "Global" 7-Pop Tox | Human | Serum-free | K2SF-7P-8HH | 8-Plate/1 Kit | |
HALO-Tox HT™
A Fast and Easy "Global" Fluorescence Assay to Monitor Lympho-Hematopoietic Reconstitution After Transplantation
| Cell Population | Species | Formulation | Catalog Number | Quantity | |
|---|---|---|---|---|---|
| Stem Cells | |||||
| SC-HPP 2 | Mouse | Low serum | K2-HPP2-4MH | 4-Plate /1 Kit | |
| SC-GEMM 1 | Mouse | Low serum | K2-GEMM1-4MH | 4-Plate /1 Kit | |
| Stem Cells | |||||
| SC-HPP 2 | Mouse | Serum-free | K2SF-HPP2-4MH | 4-Plate /1 Kit | |
| SC-GEMM 1 | Mouse | Serum-free | K2SF-GEMM1-4MH | 4-Plate /1 Kit | |
| Hematopoietic Progenitor Cells | |||||
| P-BFU 1 | Mouse | Low serum | K2-BFU1-4MH | 4-Plate /1 Kit | |
| P-GM 1 | Mouse | Low serum | K2-GM1-4MH | 4-Plate /1 Kit | |
| P-Mk 1 | Mouse | Low serum | K2-Mk1-4MH | 4-Plate /1 Kit | |
| Hematopoietic Progenitor Cells | |||||
| P-BFU 1 | Mouse | Serum-free | K2SF-BFU1-4MH | 4-Plate /1 Kit | |
| P-GM 1 | Mouse | Serum-free | K2SF-GM1-4MH | 4-Plate /1 Kit | |
| P-Mk 1 | Mouse | Serum-free | K2SF-Mk1-4MH | 4-Plate /1 Kit | |
| Lymphopoietic Progenitor Cells | |||||
| P-Tcell 1 | Mouse | Low serum | K2-T3-4MH | 4-Plate /1 Kit | |
| P-Bcell 3 | Mouse | Low serum | K2-B3-4MH | 4-Plate /1 Kit | |
| Lymphopoietic Progenitor Cells | |||||
| P-Tcell 1 | Mouse | Low serum | K2-T3-4MH | 4-Plate /1 Kit | |
| P-Bcell 3 | Mouse | Low serum | K2-B3-4MH | 4-Plate /1 Kit | |
| No Growth Factors Added | |||||
| Any | Mouse | Low serum | K2-0-4MH | 4-Plate /1 Kit | |
| Any | Mouse | Serum-free | K2SF-0-4MH | 4-Plate /1 Kit | |
| "Global" Research Assays | |||||
| "Global" 4-Pop Tox | Mouse | Low serum | K2-4P-5MH | 5-Plate/1 Kit | |
| "Global" 5-Pop Tox | Mouse | Low serum | K2-5P-6MH | 6-Plate/1 Kit | |
| "Global" 7-Pop Tox | Mouse | Low serum | K2-7P-8MH | 8-Plate/1 Kit | |
| "Global" Research Assays | |||||
| "Global" 4-Pop Tox | Mouse | Serum-free | K2SF-4P-5MH | 5-Plate/1 Kit | |
| "Global" 5-Pop Tox | Mouse | Serum-free | K2SF-5P-6MH | 6-Plate/1 Kit | |
| "Global" 7-Pop Tox | Mouse | Serum-free | K2SF-7P-8MH | 8-Plate/1 Kit | |
HALO-Tox HT™
A Fast and Easy "Global" Fluorescence Assay to Monitor Lympho-Hematopoietic Reconstitution After Transplantation
| Cell Population | Species | Formulation | Catalog Number | Quantity | |
|---|---|---|---|---|---|
| Stem Cells | |||||
| SC-HPP 2 | Primate | Low serum | K2-HPP2-4PrH | 4-Plate /1 Kit | |
| SC-GEMM 1 | Primate | Low serum | K2-GEMM1-4PrH | 4-Plate /1 Kit | |
| Stem Cells | |||||
| SC-HPP 2 | Primate | Serum-free | K2SF-HPP2-4PrH | 4-Plate /1 Kit | |
| SC-GEMM 1 | Primate | Serum-free | K2SF-GEMM1-4PrH | 4-Plate /1 Kit | |
| Hematopoietic Progenitor Cells | |||||
| P-BFU 1 | Primate | Low serum | K2-BFU1-4PrH | 4-Plate /1 Kit | |
| P-GM 1 | Primate | Low serum | K2-GM1-4PrH | 4-Plate /1 Kit | |
| P-Mk 1 | Primate | Low serum | K2-Mk1-4PrH | 4-Plate /1 Kit | |
| Hematopoietic Progenitor Cells | |||||
| P-BFU 1 | Primate | Serum-free | K2SF-BFU1-4PrH | 4-Plate /1 Kit | |
| P-GM 1 | Primate | Serum-free | K2SF-GM1-4PrH | 4-Plate /1 Kit | |
| P-Mk 1 | Primate | Serum-free | K2SF-Mk1-4PrH | 4-Plate /1 Kit | |
| Lymphopoietic Progenitor Cells | |||||
| P-Tcell 1 | Primate | Low serum | K2-T1-4PrH | 4-Plate /1 Kit | |
| P-Bcell 1 | Primate | Low serum | K2-B1-4PrH | 4-Plate /1 Kit | |
| Lymphopoietic Progenitor Cells | |||||
| P-Tcell 1 | Primate | Low serum | K2-T1-4PrH | 4-Plate /1 Kit | |
| P-Bcell 1 | Primate | Low serum | K2-B1-4PrH | 4-Plate /1 Kit | |
| No Growth Factors Added | |||||
| Any | Primate | Low serum | K2-0-4PrH | 4-Plate /1 Kit | |
| Any | Primate | Serum-free | K2SF-0-4PrH | 4-Plate /1 Kit | |
| "Global" Research Assays | |||||
| "Global" 4-Pop Tox | Primate | Low serum | K2-4P-5PrH | 5-Plate/1 Kit | |
| "Global" 5-Pop Tox | Primate | Low serum | K2-5P-6PrH | 6-Plate/1 Kit | |
| "Global" 7-Pop Tox | Primate | Low serum | K2-7P-8PrH | 8-Plate/1 Kit | |
| "Global" Research Assays | |||||
| "Global" 4-Pop Tox | Primate | Serum-free | K2SF-4P-5PrH | 5-Plate/1 Kit | |
| "Global" 5-Pop Tox | Primate | Serum-free | K2SF-5P-6PrH | 6-Plate/1 Kit | |
| "Global" 7-Pop Tox | Primate | Serum-free | K2SF-7P-8PrH | 8-Plate/1 Kit | |
HALO-Tox HT™
A Fast and Easy "Global" Fluorescence Assay to Monitor Lympho-Hematopoietic Reconstitution After Transplantation
| Cell Population | Species | Formulation | Catalog Number | Quantity | |
|---|---|---|---|---|---|
| Stem Cells | |||||
| SC-HPP 2 | Rat | Low serum | K2-HPP2-4RH | 4-Plate /1 Kit | |
| SC-GEMM 1 | Rat | Low serum | K2-GEMM1-4RH | 4-Plate /1 Kit | |
| Hematopoietic Progenitor Cells | |||||
| P-BFU 1 | Rat | Low serum | K2-BFU1-4RH | 4-Plate /1 Kit | |
| P-GM 1 | Rat | Low serum | K2-GM1-4RH | 4-Plate /1 Kit | |
| P-Mk 1 | Rat | Low serum | K2-Mk1-4RH | 4-Plate /1 Kit | |
| Lymphopoietic Progenitor Cells | |||||
| P-Tcell 1 | Rat | Low serum | K2-T1-4RH | 4-Plate /1 Kit | |
| P-Bcell 1 | Rat | Low serum | K2-B1-4RH | 4-Plate /1 Kit | |
| No Growth Factors Added | |||||
| Any | Rat | Low serum | K2-0-4RH | 4-Plate /1 Kit | |
| "Global" Research Assays | |||||
| "Global" 4-Pop Tox | Rat | Low serum | K2-4P-5RH | 5-Plate/1 Kit | |
| "Global" 5-Pop Tox | Rat | Low serum | K2-5P-6RH | 6-Plate/1 Kit | |
| "Global" 7-Pop Tox | Rat | Low serum | K2-7P-8RH | 8-Plate/1 Kit | |
HALO-Tox HT™
A Fast and Easy "Global" Fluorescence Assay to Monitor Lympho-Hematopoietic Reconstitution After Transplantation
| Cell Population | Species | Formulation | Catalog Number | Quantity | |
|---|---|---|---|---|---|
| Stem Cells | |||||
| SC-HPP 2 | Dog | Low serum | K2-HPP2-4CH | 4-Plate /1 Kit | |
| SC-GEMM 1 | Dog | Low serum | K2-GEMM1-4CH | 4-Plate /1 Kit | |
| Hematopoietic Progenitor Cells | |||||
| P-BFU 1 | Dog | Low serum | K2-BFU1-4CH | 4-Plate /1 Kit | |
| P-GM 1 | Dog | Low serum | K2-GM1-4CH | 4-Plate /1 Kit | |
| P-Mk 1 | Dog | Low serum | K2-Mk1-4CH | 4-Plate /1 Kit | |
| Lymphopoietic Progenitor Cells | |||||
| P-Tcell 1 | Dog | Low serum | K2-T1-4CH | 4-Plate /1 Kit | |
| P-Bcell 1 | Dog | Low serum | K2-B1-4CH | 4-Plate /1 Kit | |
| No Growth Factors Added | |||||
| Any | Dog | Low serum | K2-0-4CH | 4-Plate /1 Kit | |
| "Global" Research Assays | |||||
| "Global" 4-Pop Tox | Dog | Low serum | K2-4P-5CH | 5-Plate/1 Kit | |
| "Global" 5-Pop Tox | Dog | Low serum | K2-5P-6CH | 6-Plate/1 Kit | |
| "Global" 7-Pop Tox | Dog | Low serum | K2-7P-8CH | 8-Plate/1 Kit | |
Preferred Cell Systems™ is the only company, worldwide, that provides HALO®-Tox HT, the most advanced in vitro lympho-hematotoxicity assays. Originally developed and a flagship assay of HemoGenix® Contract Research Servives, HALO®-Tox HT provides academia, governmental and biopharmaceutical companies the ability to perform lympho-hematotoxicity high-throughput screening and testing in-house. This page also includes other reagents and assays that might be useful for lympho-hematotoxicity testing.
Download the HALO®-Tox HT Flyer
Benefits of Using HALO®-Tox HT for Bone Marrow/Stem Cell Hematotoxicity Testing
- Predictive in vitro to in vivo surrogate assay for human and animal hematotoxicity/hemotoxicity testing.
- Predictive in vitro stem and progenitor hematotoxicity screening and testing platform for all stages of drug development, including patient monitoring during clinical trials.
- Predictive in vitro stem and progenitor hematotoxicity screening and testing for xenobiotic agents.
- Incorporation into routine high throughput ADME/Tox screening.
- Drug / compound ranking according to species or cell population sensitivity.
- Gene targeting and expression for new drug development and therapies, e.g. CRISPR-Cas9.
- Incorporates proven, standardized and validated ATP bioluminescence technology, the most sensitive signal detection system available.
- All assay kits include everything needed to culture the required cell population and all reagents to calibrate and standardize the assay as well as 96-well plates. Assay kits are also available with 384-well plates upon request.
- Measurement assurance parameters included to ensure the assay is working correctly.
- Reliably compare results over time and between drug lots.
- Highly predictive with greater than 80% concordance between in vitro and in vivo results (Toxicol Lett. 2009; 188:98-103).
- 3Rs Alternative Assay Platform for Reduction, Refinement and Replacement of animal testing.
- Rapid turnaround: results in 5 days for human and non-human primate cells, and 4 days for other animal cells.
- Validated according to FDA Bioanalytical Method Validation Guidelines and against the Registry of Cytotoxicity Prediction Model as a cytotoxicity assay.
- Estimate in vivo starting doses and maximum tolerated doses (MTD) from in vitro IC values.
- Incorporates Suspension Expansion Culture™ (SEC™) technology for the most advanced and reliable hematotoxicity assay available.
- Designed for multiplexing with other assays.
- Simple and time efficient.
All ATP bioluminescence assays kits from Preferred Cell Systems™ include an ATP standard(s) and controls. Performing the ATP standard curve and controls is highly recommended. The controls are used to calibrate the luminescence plate reader and the ATP standard is used to standardize the assay. The values obtained from the standard curve and controls (see diagram below) are then compared to the measurement assurance parameters that are provided in the instruction manual. If the values obtained are within the ranges provided by the measurement assurance parameters, you, the user, have successfully performed the proficiency testing to ensure that you have not only performed the assay correctly, but that the results you obtain can be trusted.
No additional proficiency testing is required if the calibration and standardization procedure is performed. The values you obtain from each calibration and standardization can be logged and used for certification that the assay has been performed correctly and that the results are trustworthy.
Species
No additional proficiency testing is required if the calibration and standardization procedure is performed. The values you obtain from each calibration and standardization can be logged and used for certification that the assay has been performed correctly and that the results are trustworthy.
- Human
- Non-human primate (Cynomologus and Rhesus)
- Dog
- Rat
- Mouse
- Horse, pig and sheep also available as custom orders
- Bone marrow; human and animal tissues
- Peripheral blood; human and animal tissues
- Umbilical cord blood; human and some animals
- Spleen; rodents
- Fetal and adult liver; rodents
- Yolk sac; rodents
Stem Cells
€¢ SC-HPP 2: EPO, GM-CSF, IL-2, IL-3, Il-6, IL-7, SCF, TPO, Flt3-L
€¢ SC-GEMM 1: EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L
Progenitor Cells
€¢ P- BFU 1: EPO, IL-3, SCF
€¢ P-GM 1: GM-CSF, IL-3, SCF
€¢ P-Mk 1: TPO, IL-3, SCF
Lymphopoietic Cells
€¢ P-Tcell 1: IL-2 (requires co-stimulators, primate, dog, rat, mouse only)
€¢ P-Tcell 3: IL-2 co-stimulators CD3 CD28 (human only)
€¢ P-Bcell 1: IL-7 (requires co-stimulators, primate, dog, rat only)
€¢ P-Bcell 3: Cocktail includes IgD-Dex and cytokines (human and mouse)
No growth factors: Add growth factors/cytokines of choice.
Cell Populations for "Global" Hematotoxicity Testing
4 Population Hemotoxicity Assay Kits (4 Pop)
- SC-GEMM 1
- P-BFU 1
- P-GM 1
- P-Mk 1
- Background control
5 Population Hematotoxicity Assay Kits (5 Pop)
- SC-HPP 2
- SC-GEMM 1
- P-BFU 1
- P-GM 1
- P-Mk 1
- Background control
7 Population Lympho-Hematotoxicity Assay Kits (7 Pop)
- SC-HPP 2
- SC-GEMM 1
- P-BFU 1
- P-GM 1
- P-Mk 1
- P-Tcell 1 or 3 (depending on species)
- P-Bcell 1 or 3 (depending on species)
- Background control
For Research Use Only.
Luminescence or multimode plate reader with "glow" luminescence measuring capability.
Assay Kit Contents
Luminescence or multimode plate reader with "glow" luminescence measuring capability.
- HALO®-Tox HT Master Mix
- ATP standard
- ATP high and low controls
- ATP Enumeration Reagent
- Sterile, 96-well plates
- Non-sterile, 96-well plates
For Research Use Only.
Luminescence or multimode plate reader with "glow" luminescence measuring capability.
HemaToxFlow™ and Other In Vitro Reagents and Assays for Hematotoxicity Testing
Luminescence or multimode plate reader with "glow" luminescence measuring capability.
For studies that might involve cytotoxicity at the cell differentiation level rather than at the cell proliferation level, Preferred Cell Systems™ offers HemaToxFlow™, a flow cytometric toxicity assay that is more quantitative and better suited than the CFC/CFU assay described below.
Prior to the introduction of HALO®-Tox HT, the methylcellulose colony-forming cell or unit (CFC/CFU) assay was used to study potential toxicity. The CFC/CFU assay is still used today, despite its many flaws and its inability to be used for high-throughput toxicity screening. Nevertheless, the CFC/CFU assay may be preferred by investigators who want to study hematopoietic differentiation as a response to potentially toxic compounds. Preferred Cell Systems™ offers three CFC/CFU formats for this purpose and are listed below and accessed on other pages of our website.
In addition, Preferred Cell Systems™ also offers CellExpand™ Reagents that are used to culture and expand different hematopoietic cell populations from different species and use other readouts for hematotoxicity testing.
HALO®-Tox HT Quick Guide
Prior to the introduction of HALO®-Tox HT, the methylcellulose colony-forming cell or unit (CFC/CFU) assay was used to study potential toxicity. The CFC/CFU assay is still used today, despite its many flaws and its inability to be used for high-throughput toxicity screening. Nevertheless, the CFC/CFU assay may be preferred by investigators who want to study hematopoietic differentiation as a response to potentially toxic compounds. Preferred Cell Systems™ offers three CFC/CFU formats for this purpose and are listed below and accessed on other pages of our website.
In addition, Preferred Cell Systems™ also offers CellExpand™ Reagents that are used to culture and expand different hematopoietic cell populations from different species and use other readouts for hematotoxicity testing.
- Methylcellulose Colony-Forming Assays
- Cell Expansion Media
- CellExpand™: Cell expansion reagents for multiple cell populations and species.
Please note that HALO®-Tox HT "Global" 4-, 5- and 7-Population In Vitro Toxicity Assays are performed in the same manner as shown below, but using separate culture plates for each cell population.
Technical Manual, CoAs, SDS Sheets
Download the HALO®-Tox HT Technical Manual
Download the ATP Optimization Kit Protocol for First-Time Users
Download the Luminometer Setup and RLU to ATP Conversion Protocol
Download SDS for SEC Media for Kits
Download ATP ER SDS sheet
Download ATP Substrate and Buffer SDS sheet
Download ATP Standard and Control SDS sheet
Download Certificate of Analysis (CoAs) for ATP Enumeration Reagent (ATP-ER)
Download Certificate of Analysis for ATP Standards
Download Certificate of Analysis for ATP Controls
Download Certificate of Analysis of ATP Reconstitution Reagent
Download Certificate of Analysis for Sterile 96-Well Plates
Download Certificate of Analysis for Non-Sterile, 96-Well Plates
Download Certificate of Analysis for Sealing Films
Download Certificate of Analysis for IMDM
Download the ATP Optimization Kit Protocol for First-Time Users
Download the Luminometer Setup and RLU to ATP Conversion Protocol
Download SDS for SEC Media for Kits
Download ATP ER SDS sheet
Download ATP Substrate and Buffer SDS sheet
Download ATP Standard and Control SDS sheet
Download Certificate of Analysis (CoAs) for ATP Enumeration Reagent (ATP-ER)
Download Certificate of Analysis for ATP Standards
Download Certificate of Analysis for ATP Controls
Download Certificate of Analysis of ATP Reconstitution Reagent
Download Certificate of Analysis for Sterile 96-Well Plates
Download Certificate of Analysis for Non-Sterile, 96-Well Plates
Download Certificate of Analysis for Sealing Films
Download Certificate of Analysis for IMDM