HALO®-Tox HT

A Standardized and Validated In Vitro
ATP Bioluminescence Lympho-Hematotoxicity / 
Bone Marrow Toxicity Testing and
High-Throughput Screening Platform

 

 

HALO-Tox HT™

A Fast and Easy "Global" Fluorescence Assay to Monitor Lympho-Hematopoietic Reconstitution After Transplantation

Cell Population Species  Formulation Catalog Number Quantity
Stem Cells
SC-HPP 2 Human Low serum K2-HPP2-4HH 4-Plate /1 Kit
SC-GEMM 1 Human Low serum K2-GEMM1-4HH 4-Plate /1 Kit
Stem Cells
SC-HPP 2 Human Serum-free K2SF-HPP2-4HH 4-Plate /1 Kit
SC-GEMM 1 Human Serum-free K2SF-GEMM1-4HH 4-Plate /1 Kit
Hematopoietic Progenitor Cells
P-BFU 1 Human Low serum K2-BFU1-4HH 4-Plate /1 Kit
P-GM 1 Human Low serum K2-GM1-4HH 4-Plate /1 Kit
P-Mk 1 Human Low serum K2-Mk1-4HH 4-Plate /1 Kit
Hematopoietic Progenitor Cells
P-BFU 1 Human Serum-free K2SF-BFU1-4HH 4-Plate /1 Kit
P-GM 1 Human Serum-free K2SF-GM1-4HH 4-Plate /1 Kit
P-Mk 1 Human Serum-free K2SF-Mk1-4HH 4-Plate /1 Kit
Lymphopoietic Progenitor Cells
P-Tcell 3 Human Low serum K2-T3-4HH 4-Plate /1 Kit
P-Bcell 3 Human Low serum K2-B3-4HH 4-Plate /1 Kit
Lymphopoietic Progenitor Cells
P-Tcell 3 Human Low serum K2-T3-4HH 4-Plate /1 Kit
P-Bcell 3 Human Low serum K2-B3-4HH 4-Plate /1 Kit
No Growth Factors Added
Any Human Low serum K2-0-4HH 4-Plate /1 Kit
Any Human Serum-free K2SF-0-4HH 4-Plate /1 Kit
"Global" Research Assays
"Global" 4-Pop Tox Human Low serum K2-4P-5HH 5-Plate/1 Kit
"Global" 5-Pop Tox Human Low serum K2-5P-6HH 6-Plate/1 Kit
"Global" 7-Pop Tox Human Low serum K2-7P-8HH 8-Plate/1 Kit
"Global" Research Assays
"Global" 4-Pop Tox Human Serum-free K2SF-4P-5HH 5-Plate/1 Kit
"Global" 5-Pop Tox Human Serum-free K2SF-5P-6HH 6-Plate/1 Kit
"Global" 7-Pop Tox Human Serum-free K2SF-7P-8HH 8-Plate/1 Kit

HALO-Tox HT™

A Fast and Easy "Global" Fluorescence Assay to Monitor Lympho-Hematopoietic Reconstitution After Transplantation

Cell Population Species  Formulation Catalog Number Quantity
Stem Cells
SC-HPP 2 Mouse Low serum K2-HPP2-4MH 4-Plate /1 Kit
SC-GEMM 1 Mouse Low serum K2-GEMM1-4MH 4-Plate /1 Kit
Stem Cells
SC-HPP 2 Mouse Serum-free K2SF-HPP2-4MH 4-Plate /1 Kit
SC-GEMM 1 Mouse Serum-free K2SF-GEMM1-4MH 4-Plate /1 Kit
Hematopoietic Progenitor Cells
P-BFU 1 Mouse Low serum K2-BFU1-4MH 4-Plate /1 Kit
P-GM 1 Mouse Low serum K2-GM1-4MH 4-Plate /1 Kit
P-Mk 1 Mouse Low serum K2-Mk1-4MH 4-Plate /1 Kit
Hematopoietic Progenitor Cells
P-BFU 1 Mouse Serum-free K2SF-BFU1-4MH 4-Plate /1 Kit
P-GM 1 Mouse Serum-free K2SF-GM1-4MH 4-Plate /1 Kit
P-Mk 1 Mouse Serum-free K2SF-Mk1-4MH 4-Plate /1 Kit
Lymphopoietic Progenitor Cells
P-Tcell 1 Mouse Low serum K2-T3-4MH 4-Plate /1 Kit
P-Bcell 3 Mouse Low serum K2-B3-4MH 4-Plate /1 Kit
Lymphopoietic Progenitor Cells
P-Tcell 1 Mouse Low serum K2-T3-4MH 4-Plate /1 Kit
P-Bcell 3 Mouse Low serum K2-B3-4MH 4-Plate /1 Kit
No Growth Factors Added
Any Mouse Low serum K2-0-4MH 4-Plate /1 Kit
Any Mouse Serum-free K2SF-0-4MH 4-Plate /1 Kit
"Global" Research Assays
"Global" 4-Pop Tox Mouse Low serum K2-4P-5MH 5-Plate/1 Kit
"Global" 5-Pop Tox Mouse Low serum K2-5P-6MH 6-Plate/1 Kit
"Global" 7-Pop Tox Mouse Low serum K2-7P-8MH 8-Plate/1 Kit
"Global" Research Assays
"Global" 4-Pop Tox Mouse Serum-free K2SF-4P-5MH 5-Plate/1 Kit
"Global" 5-Pop Tox Mouse Serum-free K2SF-5P-6MH 6-Plate/1 Kit
"Global" 7-Pop Tox Mouse Serum-free K2SF-7P-8MH 8-Plate/1 Kit

HALO-Tox HT™

A Fast and Easy "Global" Fluorescence Assay to Monitor Lympho-Hematopoietic Reconstitution After Transplantation

Cell Population Species  Formulation Catalog Number Quantity
Stem Cells
SC-HPP 2 Primate Low serum K2-HPP2-4PrH 4-Plate /1 Kit
SC-GEMM 1 Primate Low serum K2-GEMM1-4PrH 4-Plate /1 Kit
Stem Cells
SC-HPP 2 Primate Serum-free K2SF-HPP2-4PrH 4-Plate /1 Kit
SC-GEMM 1 Primate Serum-free K2SF-GEMM1-4PrH 4-Plate /1 Kit
Hematopoietic Progenitor Cells
P-BFU 1 Primate Low serum K2-BFU1-4PrH 4-Plate /1 Kit
P-GM 1 Primate Low serum K2-GM1-4PrH 4-Plate /1 Kit
P-Mk 1 Primate Low serum K2-Mk1-4PrH 4-Plate /1 Kit
Hematopoietic Progenitor Cells
P-BFU 1 Primate Serum-free K2SF-BFU1-4PrH 4-Plate /1 Kit
P-GM 1 Primate Serum-free K2SF-GM1-4PrH 4-Plate /1 Kit
P-Mk 1 Primate Serum-free K2SF-Mk1-4PrH 4-Plate /1 Kit
Lymphopoietic Progenitor Cells
P-Tcell 1 Primate Low serum K2-T1-4PrH 4-Plate /1 Kit
P-Bcell 1 Primate Low serum K2-B1-4PrH 4-Plate /1 Kit
Lymphopoietic Progenitor Cells
P-Tcell 1 Primate Low serum K2-T1-4PrH 4-Plate /1 Kit
P-Bcell 1 Primate Low serum K2-B1-4PrH 4-Plate /1 Kit
No Growth Factors Added
Any Primate Low serum K2-0-4PrH 4-Plate /1 Kit
Any Primate Serum-free K2SF-0-4PrH 4-Plate /1 Kit
"Global" Research Assays
"Global" 4-Pop Tox Primate Low serum K2-4P-5PrH 5-Plate/1 Kit
"Global" 5-Pop Tox Primate Low serum K2-5P-6PrH 6-Plate/1 Kit
"Global" 7-Pop Tox Primate Low serum K2-7P-8PrH 8-Plate/1 Kit
"Global" Research Assays
"Global" 4-Pop Tox Primate Serum-free K2SF-4P-5PrH 5-Plate/1 Kit
"Global" 5-Pop Tox Primate Serum-free K2SF-5P-6PrH 6-Plate/1 Kit
"Global" 7-Pop Tox Primate Serum-free K2SF-7P-8PrH 8-Plate/1 Kit

HALO-Tox HT™

A Fast and Easy "Global" Fluorescence Assay to Monitor Lympho-Hematopoietic Reconstitution After Transplantation

Cell Population Species  Formulation Catalog Number Quantity
Stem Cells
SC-HPP 2 Rat Low serum K2-HPP2-4RH 4-Plate /1 Kit
SC-GEMM 1 Rat Low serum K2-GEMM1-4RH 4-Plate /1 Kit
Hematopoietic Progenitor Cells
P-BFU 1 Rat Low serum K2-BFU1-4RH 4-Plate /1 Kit
P-GM 1 Rat Low serum K2-GM1-4RH 4-Plate /1 Kit
P-Mk 1 Rat Low serum K2-Mk1-4RH 4-Plate /1 Kit
Lymphopoietic Progenitor Cells
P-Tcell 1 Rat Low serum K2-T1-4RH 4-Plate /1 Kit
P-Bcell 1 Rat Low serum K2-B1-4RH 4-Plate /1 Kit
No Growth Factors Added
Any Rat Low serum K2-0-4RH 4-Plate /1 Kit
"Global" Research Assays
"Global" 4-Pop Tox Rat Low serum K2-4P-5RH 5-Plate/1 Kit
"Global" 5-Pop Tox Rat Low serum K2-5P-6RH 6-Plate/1 Kit
"Global" 7-Pop Tox Rat Low serum K2-7P-8RH 8-Plate/1 Kit

HALO-Tox HT™

A Fast and Easy "Global" Fluorescence Assay to Monitor Lympho-Hematopoietic Reconstitution After Transplantation

Cell Population Species  Formulation Catalog Number Quantity
Stem Cells
SC-HPP 2 Dog Low serum K2-HPP2-4CH 4-Plate /1 Kit
SC-GEMM 1 Dog Low serum K2-GEMM1-4CH 4-Plate /1 Kit
Hematopoietic Progenitor Cells
P-BFU 1 Dog Low serum K2-BFU1-4CH 4-Plate /1 Kit
P-GM 1 Dog Low serum K2-GM1-4CH 4-Plate /1 Kit
P-Mk 1 Dog Low serum K2-Mk1-4CH 4-Plate /1 Kit
Lymphopoietic Progenitor Cells
P-Tcell 1 Dog Low serum K2-T1-4CH 4-Plate /1 Kit
P-Bcell 1 Dog Low serum K2-B1-4CH 4-Plate /1 Kit
No Growth Factors Added
Any Dog Low serum K2-0-4CH 4-Plate /1 Kit
"Global" Research Assays
"Global" 4-Pop Tox Dog Low serum K2-4P-5CH 5-Plate/1 Kit
"Global" 5-Pop Tox Dog Low serum K2-5P-6CH 6-Plate/1 Kit
"Global" 7-Pop Tox Dog Low serum K2-7P-8CH 8-Plate/1 Kit

 

HALO®-Tox HT and HALO®-Tox HT "Global": Standardized and Validated Lympho-Hematotoxity Assays
 

Preferred Cell Systems™ is the only company, worldwide, that provides HALO®-Tox HT, the most advanced in vitro lympho-hematotoxicity assays. Originally developed and a flagship assay of HemoGenix® Contract Research Servives, HALO®-Tox HT provides academia, governmental and biopharmaceutical companies the ability to perform lympho-hematotoxicity high-throughput screening and testing in-house. This page also includes other reagents and assays that might be useful for lympho-hematotoxicity testing.

Download the HALO®-Tox HT Flyer 

 

  • Predictive in vitro to in vivo surrogate assay for human and animal hematotoxicity/hemotoxicity testing.
  • Predictive in vitro stem and progenitor hematotoxicity screening and testing platform for all stages of drug development, including patient monitoring during clinical trials.
  • Predictive in vitro stem and progenitor hematotoxicity screening and testing for xenobiotic agents.
  • Incorporation into routine high throughput ADME/Tox screening.
  • Drug / compound ranking according to species or cell population sensitivity.
  • Gene targeting and expression for new drug development and therapies, e.g. CRISPR-Cas9.
  • Incorporates proven, standardized and validated ATP bioluminescence technology, the most sensitive signal detection system available.
  • All assay kits include everything needed to culture the required cell population and all reagents to calibrate and standardize the assay as well as 96-well plates. Assay kits are also available with 384-well plates upon request.
  • Measurement assurance parameters included to ensure the assay is working correctly.
  • Reliably compare results over time and between drug lots.
  • Highly predictive with greater than 80% concordance between in vitro and in vivo results (Toxicol Lett. 2009; 188:98-103).
  • 3Rs Alternative Assay Platform for Reduction, Refinement and Replacement of animal testing.
  • Rapid turnaround: results in 5 days for human and non-human primate cells, and 4 days for other animal cells.
  • Validated according to FDA Bioanalytical Method Validation Guidelines and against the Registry of Cytotoxicity Prediction Model as a cytotoxicity assay.
  • Estimate in vivo starting doses and maximum tolerated doses (MTD) from in vitro IC values.
  • Incorporates Suspension Expansion Culture™ (SEC™) technology for the most advanced and reliable hematotoxicity assay available.
  • Designed for multiplexing with other assays.
  • Simple and time efficient.

Principle of ATP Bioluminescence Cell Proliferation/Cytotoxicity Assays

All ATP bioluminescence assays kits from Preferred Cell Systems™ include an ATP standard(s) and controls.  Performing the ATP standard curve and controls is highly recommended. The controls are used to calibrate the luminescence plate reader and the ATP standard is used to standardize the assay. The values obtained from the standard curve and controls (see diagram below) are then compared to the measurement assurance parameters that are provided in the instruction manual. If the values obtained are within the ranges provided by the measurement assurance parameters, you, the user, have successfully performed the proficiency testing to ensure that you have not only performed the assay correctly, but that the results you obtain can be trusted.

No additional proficiency testing is required if the calibration and standardization procedure is performed. The values you obtain from each calibration and standardization can be logged and used for certification that the assay has been performed correctly and that the results are trustworthy.

Calibration and standardization of an ATP bioluminescence assay from Preferred Cell Systems™ - Measurement Assurance
 
  • Human
  • Non-human primate (Cynomologus and Rhesus)
  • Dog
  • Rat
  • Mouse
  • Horse, pig and sheep also available as custom orders
  • Bone marrow; human and animal tissues
  • Peripheral blood; human and animal tissues
  • Umbilical cord blood; human and some animals
  • Spleen; rodents
  • Fetal and adult liver; rodents
  • Yolk sac; rodents

Stem Cells

SC-HPP 2: EPO, GM-CSF, IL-2, IL-3, Il-6, IL-7, SCF, TPO, Flt3-L

SC-GEMM 1: EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L

Progenitor Cells

• P- BFU 1: EPO, IL-3, SCF

P-GM 1: GM-CSF, IL-3, SCF

P-Mk 1: TPO, IL-3, SCF

Lymphopoietic Cells

•  P-Tcell 1: IL-2 (requires co-stimulators, primate, dog, rat, mouse only)

•  P-Tcell 3: IL-2 co-stimulators CD3 CD28 (human only)

•  P-Bcell 1: IL-7 (requires co-stimulators, primate, dog, rat only)

•  P-Bcell 3: Cocktail includes IgD-Dex and cytokines (human and mouse)

 

No growth factors: Add growth factors/cytokines of choice.

 

 

Cell Populations for "Global" Hematotoxicity Testing

 

4 Population Hemotoxicity Assay Kits (4 Pop)

  • SC-GEMM 1
  • P-BFU 1
  • P-GM 1
  • P-Mk 1
  • Background control

 

5 Population Hematotoxicity Assay Kits (5 Pop)

  • SC-HPP 2
  • SC-GEMM 1
  • P-BFU 1
  • P-GM 1
  • P-Mk 1
  • Background control
 

7 Population Lympho-Hematotoxicity Assay Kits (7 Pop)

  • SC-HPP 2
  • SC-GEMM 1
  • P-BFU 1
  • P-GM 1
  • P-Mk 1
  • P-Tcell 1 or 3 (depending on species)
  • P-Bcell 1 or 3 (depending on species)
  • Background control
For Research Use Only.

Luminescence or multimode plate reader with "glow" luminescence measuring capability.
  • HALO®-Tox HT Master Mix
  • ATP standard
  • ATP high and low controls
  • ATP Enumeration Reagent
  • Sterile, 96-well plates
  • Non-sterile, 96-well plates
For Research Use Only.

Luminescence or multimode plate reader with "glow" luminescence measuring capability.
For studies that might involve cytotoxicity at the cell differentiation level rather than at the cell proliferation level, Preferred Cell Systems™ offers HemaToxFlow™, a flow cytometric toxicity assay that is more quantitative and better suited than the CFC/CFU assay described below.

Prior to the introduction of HALO®-Tox HT, the methylcellulose colony-forming cell or unit (CFC/CFU) assay was used to study potential toxicity. The CFC/CFU assay is still used today, despite its many flaws and its inability to be used for high-throughput toxicity screening. Nevertheless, the CFC/CFU assay may be preferred by investigators who want to study hematopoietic differentiation as a response to potentially toxic compounds. Preferred Cell Systems™ offers three CFC/CFU formats for this purpose and are listed below and accessed on other pages of our website.

In addition, Preferred Cell Systems™ also offers CellExpand™ Reagents that are used to culture and expand different hematopoietic cell populations from different species and use other readouts for hematotoxicity testing.
 
  • Methylcellulose Colony-Forming Assays
    • CAMEO™-4: A quadruplicate, miniaturized CFC assay for multiple populations and species.
    • CAMEO™-96: A dual endpoint CFC, 96-well plate assay that allows colony enumeration and measures total colony proliferation for bone marrow toxicity/stem cell hematotoxicity. 
    • ColonyGro: The traditional CFC assay.
  • Cell Expansion Media
    • CellExpand™: Cell expansion reagents for multiple cell populations and species.
 
Please note that HALO®-Tox HT "Global" 4-, 5- and 7-Population In Vitro Toxicity Assays are performed in the same manner as shown below, but using separate culture plates for each cell population.
HALO®-Tox HT: The Advanced High Throughput Screening and Testing Hematotoxicity Platform

 

Download the HALO®-Tox HT Technical Manual
Download the ATP Optimization Kit Protocol for First-Time Users
Download the Luminometer Setup and RLU to ATP Conversion Protocol
Download Certificate of Analysis (CoAs) for ATP bioluminescence components. Note: Some of CoAs will be included with the assay kits.
Download SDS for SEC Media for Kits
Download Certificate of Analysis (CoAs) for ATP Enumeration Reagent (ATP-ER)
Download Certificate of Analysis for ATP Stanadrds
Download Certificate of Analysis for ATP Controls
Download Certificate of Analysis of ATP Reconstitution Reagent
Download Certificate of Analysis for Sterile 96-Well Plates
Download Certificate of Analysis for Non-Sterile, 96-Well Plates
Download Certificate of Analysis for Sealing Films
Download Certificate of Analysis for IMDM