MSCGlo™-Tox HT

A Standardized and Validated In Vitro Bioluminescence Assay to Detect Toxicity to Mesenchymal Cells (MSC)

Cell Population	Tissue	Formulation	Catalog Number	Quantity
Mesenchymal Stem/Stromal Cells	Human	Serum-free, Humanized	KLMC-T96SF-4	4 plate/1 Kit

• Predictive in vitro to in vivo surrogate assay for human and animal MSC/MPC toxicity.
• Predictive in vitro MSC/MPC screening and testing platform for all stages of drug development. 
• Predictive in vitro MSC/MPC toxicity screening and testing for xenobiotic agents.
• Incorporation into routine high throughput ADME/Tox screening.
• Drug/ compound ranking according to species and/or cell population sensitivity.
• Regenerative drug therapy using gene targeting methodology, e.g. CRISPR-Cas9.

• High throughput, ATP bioluminescence assay to measure potential toxicity to the MSC/MPC system.
• Fully standardized with validation capability.
• A 3Rs Alternative Assay Platform for Reduction, Refinement and Replacement of animal testing.
• The only in vitro high throughput MSC toxicity assay platform available with both 96-well and 384-well plate formats.
• Rapid turnaround: results in about 3 days.
• Customized assay kits with either CRUXRUFA Human Platelet Lysate or different formulations of MSCGro™ media.
• Designed for multiplexing with other assays to produce the most amount of information from a single sample.
• Simple, time efficient and cost effective.

All ATP bioluminescence assays kits from Preferred Cell Systems™ include an ATP standard(s) and controls.  Performing the ATP standard curve and controls is highly recommended. The controls are used to calibrate the luminescence plate reader and the ATP standard is used to standardize the assay. The values obtained from the standard curve and controls (see diagram below) are then compared to the measurement assurance parameters that are provided in the instruction manual. If the values obtained are within the ranges provided by the measurement assurance parameters, you, the user, have successfully performed the proficiency testing to ensure that you have not only performed the assay correctly, but that the results you obtain can be trusted.

No additional proficiency testing is required if the calibration and standardization procedure is performed. The values you obtain from each calibration and standardization can be logged and used for certification that the assay has been performed correctly and that the results are trustworthy.


 

• Human
• Non-human primate (Cynomologus and Rhesus)
• Rat
• Mouse

MSCGlo™-Tox HT can be used with MSCs derived from fresh primary tissues such as bone marrow and umbilical cord blood or MSCs derived from other cells such as induced pluripotent stem cells (iPSC).

For Research Use Only.

Luminescence or multimode plate reader with "glow" luminescence measuring capability.

• MSCGro™ Medium of choice
• ATP standard
• ATP high and low controls
• ATP Enumeration Reagent
• Sterile, 96-well plates
• Non-sterile, 96-well plates
• Sterile, adhesive foil covers

MSCGlo™-Tox HT Assay Manual

When ordering MSCGlo™-Tox HT, click on the catalog number link in the table below to go directly to the product order page.
 

MSCGlo™-Tox HT Assay Kits

Catalog Number	Species	MSC Growth Medium Formulation
KLMC-T96SF-2	Human, Primate, Rat, Mouse	3
KLMC-T96SF-4	Human, Primate, Rat, Mouse	Human