Assay Kits for In Vitro Toxicity Testing
Preferred Cell Systems™ is the only company worldwide to provide in vitro toxicity testing assay kits that are fully standardized and validated and designed specifically for different cell types, tissues and organs.
All of the assay kits include 2 or 4, 96-well plates (bulk is also available), standards and controls and reagents to measure intracellular ATP concentrations using a luciferin/luciferase bioluminescence readout. Therefore, a luminescence plate reader is required to perform any of the in vitro toxicity assays. All assays have been standardized and validated according to FDA Bioanaytical Method Validation Guidelines. However, since reagents to calibrate and standardize the assays are included, additional in-house validation can also be perform, if required.
Preferred Cell Systems™ does not include target cells with any of the in vitro toxicity assay kits. Please contact Preferred Cell Systems™ for vendors of target cells.
Many different types of cells and tissues require their own specific plating/maintenance/growth medium. Not every medium combination can be accomodated in our assay kits. However, the in vitro toxicity assay kits are flexible so that investigators can use their own medium and protocols.
All Preferred Cell Systems™ in vitro toxicity assay kits can be adapted to multiplex with other readouts. Please contact Preferred Cell Systems™ for further information.
An Overview and Summary of all In Vitro Toxicity Assay Kit Platforms
| Cells / Tissues / Organ Types | Type of Toxicity | Name of Assay | Cell Types | Species Available |
|---|---|---|---|---|
| Lympho-hematopoietic cells (Bone marrow toxicity) |
Lympho-hematotoxicity | HALO®-Tox HT HALO®-Tox HT "Global" |
Stem cells Erythropoietic progenitors Myelomonocytic progenitors Megakaryopoietic progenitors Lymphopoietic (T- and B-cell) progenitors |
Human Primate Dog Rat Mouse |
| Immune cells | Immunotoxicity | ImmunoGLO™-Tox HT ImmunoGLO™-Tox HT TCP ImmunoGLO™-Tox HT BCP |
Functional immune cells Functional T-lymphocytes Functional B-lymphocytes |
Human Primate Dog rat Mouse |
| Liver cells | Hepatotoxicity | HepatoGLO™-Tox HT | Hepatocytes | Any |
| Heart cells | Cardiotoxicity | CardioGLO™-Tox HT | Cardiomyocytes | Any |
| Kidney cells | Renal toxicity | RenalGLO™-Tox HT | Cortex, medulla and tubule | Any |
| Skin cells | Skin toxicity | SkinGLO™-Tox HT | Epidermal | Any |
| Neural cells | Neurotoxicity | NeuroGLO™-Tox HT | Neural cells | Any |
| Intestinal cells | Intestinal toxicity | EpiGLO™-Tox HT | Intestinal epithelium | Any |
| Mesenchymal cells | Mesenchymal cell toxicity | MSCGlo™-Tox HT | Mesenchymal cells from any source | Any |
| Tumor cells | Tumor cell toxicity | CellaGLO™-Tox HT | Multiple tumnor types | Any |
| Cell lines | Transforms cell lines, including ES and iPS | STEMGlo™-Tox HT | Any cell line | Any |