The Leader in Assays to Study the Lympho-Hematopoietic System
Please refer to our Statement below regarding why Preferred Cell Systems™ has decided to remove all colony-forming assays from it's product list
Media and Cell Expansion Reagents
- HemoGro™ Media: Base serum-free and low serum for optimal growth
- CellExpand™: Cell expansion reagents for multiple populations and species. New reagents are now available for expanding T- or B-lymphopoietic cell populations.
- HemaFlow™: The Intelligent Cell Differentiation Assay to replace the CFU assay.
Non-Biased, Lympho-Hematopoietic Cell Viability and Proliferation Assays that Replace the Colony-Forming Assay
- Standardized and Validated ATP Bioluminescence Assays
- HALO®: Assays for discerning scientists. Individual assays for multiple lympho-hematopoietic cells populations from up to 9 different species, including assays for T- and B-lymphopoietic progenitor cells.
- HALO® "Global": 4-, 5- and 7-population assays that predict the "global" response of the lympho-hematopoietic system.
- Fast and Easy to Use ATP Bioluminescence Assays with Optional Standardization
- HemoGLO™: An easy and rapid ATP bioluminescence assay, equivalent to HemoFLUOR™ and HemoLIGHT™ (see below) for lympho-hematopoietic stem and progenitor cells that is similar in price to MethoCult™.
- Fast and Easy to Use Fluorescence Assays with Excellent Flow Cytometric Capability
- MultiCellGro™: Multiple lineage assay with phenotypic capability
- HemoFLUOR™: The fluorescence assay equivalent to HemoGLO™, but with better flow cytometric multiplexing capability, but similar in price to MethoCult™.
- Fast and Easy to Use Absorbance/Colorimetric Assays with Excellent Flow Cytometric Capability
- HemoLIGHT™: The absorbance/colorimetric assay equivalent to HemoGLO™ and HemoFLUOR™, but with excellent flow cytometric multiplexing capability, and similar in price to MethoCult™.
Methylcellulose Colony-Forming Assays are no longer offered by Preferred Cell Systems™: A New Era in Hematopoietic Research and Cellular Therapy
From Colony-Forming Assays to Instrument-Based Assays
The Rationale for Change
The decision to pivot from colony-forming assays to instrument-based assays was not made lightly. It was driven by several compelling factors that underscore the need for more advanced and reliable methodologies.Inherent Limitations of Colony-Forming Assays
Colony-forming assays have long been a staple in hematopoietic research. These assays involve culturing hematopoietic cells in a semi-solid medium to allow the formation of colonies, which are then counted to assess the cells' differentiation potential. Despite their widespread use, colony-forming assays are susceptible to several limitations:- Subjectivity: The interpretation of colony-forming assays can be highly subjective, with variations in colony counting and classification among different researchers.
- Variability: These assays are prone to variability due to differences in culture conditions, reagents, and operator techniques.
- Time-Consuming: Colony-forming assays require extended incubation periods, up to 14 or more days, delaying the acquisition of results.
The Advantages of Instrument-Based Assays
Instrument-based assays, in contrast, offer a host of benefits that address the shortcomings of colony-forming assays:- Objectivity: These assays leverage automated instrumentation to provide unbiased, consistent measurements, eliminating subjective interpretation.
- Precision: Instrument-based assays deliver high precision and reproducibility, ensuring reliable and trustworthy data.
- Efficiency: These assays significantly reduce the time required to obtain results, accelerating the pace of research and decision-making.
Preferred Cell Systems™' Proprietary Instrument-Based Assays
Preferred Cell Systems™ has developed a suite of proprietary instrument-based assays specifically designed for hematopoietic research and cellular therapy. These innovative assays represent a significant advancement in the field, offering enhanced accuracy and reproducibility.Key Features and Benefits
The proprietary instrument-based assays from Preferred Cell Systems™ incorporate cutting-edge technology and are optimized for a range of applications:- Non-Biased Measurements: The assays utilize automated systems to ensure non-biased data collection, minimizing human error and variability.
- High Throughput: Capable of processing large sample volumes, these assays facilitate high-throughput screening and analysis, expediting research workflows.
- Comprehensive Data Insights: The advanced instrumentation provides detailed and comprehensive data insights, enabling researchers to make informed decisions and draw robust conclusions.
Applications in Hematopoietic Research
In hematopoietic research, the proprietary instrument-based assays are poised to revolutionize the study of blood and bone marrow cells. Key applications include:- Stem Cell Characterization: Accurate identification, characterization and regulation of hematopoietic stem cells, critical for understanding their role in blood formation and regeneration.
- Drug Screening: High-throughput screening of potential therapeutics for hematopoietic, immune and other disorders, accelerating the discovery of new treatments.
- Functional Analysis: Detailed functional analysis of hematopoietic cells, providing insights into their behavior and response to various stimuli.
Advancements in Cellular Therapy
Cellular therapy is a rapidly evolving field that stands to benefit immensely from the adoption of instrument-based assays. Key advancements include:- Quality and Potency Control: Rigorous quality and potency control of cellular products, ensuring their safety, efficacy, and consistency for therapeutic use.
- Process Optimization: Enhanced process optimization for the expansion and differentiation of therapeutic cells, improving the efficiency of cell manufacturing.
- Patient-Specific Therapies: Development of patient-specific cellular therapies, tailored to individual needs and conditions, enhancing treatment outcomes.