AllColonies™

A Multi-Stem Cell, Multi-Lineage, Hematopoietic 
Methylcellulose CFU Assay

 

AllColonies™ Micro: Miniaturized, 4-well assay that includes 50 culture plates for 50 samples.

 

AllColonies™

A Multi-Stem Cell, Multi-Lineage, Hematopoietic Minaturized Methylcellulose CFU Assay

Cell Population Species Formulation Catalog Number Quantity
Multi-Stem Cells
Multi-Lineage Human Low serum AL-KC-50H 50mL
Multi-Lineage Mouse Low serum AL-KC-50M 50mL
Multi-Lineage Primate Low serum AL-KC-50Pr 50mL
Multi-Lineage Rat Low serum AL-KC-50R 50mL
Multi-Lineage Dog Low serum AL-KC-50C 50mL
AllColonies™ Traditional: 35mm Petri dish format, used for all colony-forming unit (CFU) assays.
Sufficient reagent for 44 samples.

AllColonies™

A Multi-Stem Cell, Multi-Lineage, Hematopoietic Minaturized Methylcellulose CFU Assay

Cell Population Species Formulation Catalog Number Quantity
Multi-Stem Cells
Multi-Lineage Human Low serum AL-KCO-1H 1 Kit
Multi-Lineage Mouse Low serum AL-KCO-1M 1 Kit
Multi-Lineage Primate Low serum AL-KCO-1Pr 1 Kit
Multi-Lineage Rat Low serum AL-KCO-1R 1 Kit
Multi-Lineage Dog Low serum AL-KCO-1C 1 Kit
AllColonies™ Multi: A complete assay kit using a 96-well plate format that allows manual enumeration of colonies and measurement of total cell colony proliferation to standardize the AllColonies™ assay using an ATP bioluminescence readout.

 

AllColonies™

A Multi-Stem Cell, Multi-Lineage, Hematopoietic Traditional Methylcellulose CFU Assay

Cell Population Species Formulation Catalog Number Quantity
Multi-Stem Cells
Multi-Lineage Human Low serum AL-CG-100H 100mL
Multi-Lineage Mouse Low serum AL-CG-100M 100mL
Multi-Lineage Primate Low serum AL-CG-100Pr 100mL
Multi-Lineage Rat Low serum AL-CG-100R 100mL
Multi-Lineage Dog Low serum AL-CG-100C 100mL

 

AllColonies: A Multi-Stem Cell, Multi-Lineage Methylcellulose Colony-Forming Unit (CFU) Assay in 3 Formats

 

A Multi-Stem Cell, Multi-Lineage, Hematopoietic Methylcellulose Colony-Forming Cell or Unit (CFC/CFU) Assay Available in 3 Different Formats for Research and Cellular Therapy from The Colony Experts
  • Detect multiple stem cell and multi-lineage colonies in a single culture.
  • Any type of hematopoietic research requiring clonal growth of proliferating cell populations.
  • Any hematopoietic cellular therapy application requiring the simultaneous enumeration of multiple cell types in vitro
  • Includes a proprietary growth factor cocktail that allows the production of different hematopoietic stem cell colonies, (CFC-GEMM and CFC-GEM) as well as multiple progenitor cell colonies (BFU-E, CFU-E, GM-CFC, M-CFC, G-CFC, Mk-CFC and even T-CFC and B-CFC). PLEASE NOTE: The formation of colonies from different lineages depends on the source, status and degree of fractionation of the cell suspension used.
  • Available in 3 different culture formats (see below).
  • Colonies can be enumerated between day 5 and 12 of culture depending on species and tissue.
  • Colony cells can be removed from the plate and prepared for flow cytometric phenotypic analysis.
  • The proprietary growth factor and supplement cocktail used allows a greater diversity of colonies to be produced than any other competitor product.
The following are a series of colony images from cultures using the AllColonies™ Reagent. AllColonies™ produces massive colonies that are derived from very primitive lympho-hematopoietic stem cells stimulated with a proprietary cocktails of factors. The colonies produced demonstrate that the cells can be from individual lineages or multiple lineages. The size of some of the colonies is an indication of the primitiveness of the cell giving rise to the colony.

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Image 10: How many colonies are in this image?
  • Human
  • Non-human primate
  • Dog
  • Rat
  • Mouse
  • Bone marrow from all species
  • Umbilical cord blood from human tissue
  • Peripheral blood from all species
  • Mobilized peripheral blood from human tissue
  • Other hematopoietic organs and tissues from different species

AllColonies™ Traditional: 35mm Plate Format

  • 100mL AllColonies™ Reagent

AllColonies™ Micro: Miniaturized Format

  • AllColonies™ Reagent
  • 50 culture plates

AllColonies™ Multi: 96-Well Plate Format

  • AllColonies™ Reagent
  • Sterile 96-well plate for cell culture
  • Non-sterile 96-well plate for assay standardization
  • ATP Enumeration Reagent
  • ATP standard
  • ATP controls
  • Sterile adhesive foil to maintain unused wells sterile
For Research Use Only.

An inverted microscope with 40x and at least 100x magnification is required for all three assay formats.
A luminescence plate reader is also required for the 96-well plate format assay.
AllColonies™ Traditional, Micro or Multi: Detection of primitive hematopoietic stem cells
 
Download the AllColonies™ Traditional Manual
Download the AllColonies™ Micro Manual
Download the AllColonies™ Multi Manual
Download the Document for Luminometer Setup and RLU to ATP Conversion
Download Certificate of Analysis (CoAs) for ATP Bioluminescence Components. Note: Some CoAs will be included with assay kit
Download the SDS sheet