HemoFLUOR™

The In Vitro Standardized and Validated Bioluminescence Research Assay Platform for Lympho-Hematopoietic Cells

HemoFLUOR™

The Easy and Rapid In Vitro Research Bioluminescence Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

HemoFLUOR™

The Easy and Rapid In Vitro Research Bioluminescence Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

HemoFLUOR™

The Easy and Rapid In Vitro Research Bioluminescence Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

HemoFLUOR™

The Easy and Rapid In Vitro Research Bioluminescence Assay for Measuring Cell Viability and ProlifeDogion of Lympho-Hematopoietic Cells

HemoFLUOR™

The Easy and Rapid In Vitro Research Bioluminescence Assay for Measuring Cell Viability and ProlifeHorseion of Lympho-Hematopoietic Cells

HemoFLUOR™

The Easy and Rapid In Vitro Research Fluorescence Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

HemoFLUOR™

The Easy and Rapid In Vitro Research Bioluminescence Assay for Measuring Cell Viability and ProlifeSheepion of Lympho-Hematopoietic Cells

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How to Setup HALO®, HemoFLUOR™ or HemoLIGHT™ Hematopoietic Research Assays

How to Perform a Fluorescence-Based Readout (for HemoFLUOR™ Research Assays)

Please navigate to HALO® or HemoLIGHT™ to see how to perform an ATP bioluminescence or absorbance readout. 

• Determine viability, proliferation ability and potential.
• Compare hematopoietic cell populations from different species.
• Replace hematopoietic colony-forming cell (CFC) assay.
• Experimental transplantation models.
• Effects of growth factors/cytokines.
• In vivo to in vitro concordance.
• Gene targeting assays.

• No colony counting needed.
• Non-subjective, instrument-based and quantitative.
• Uses a single-step, resazurin (alamarBlue™) reagent that is converted to a pink/red fluorescent resorufin product (excitation filter, 560nm; emission filter, 590nm).
• Multiplex with flow cytometry allowing cells to be detected using other fluorescent labels/markers. 
• Multiplex with bioluminescence assays.
• Results in about 4-7 days depending on cell population and species.
• Simple, time efficient, cost effective and flexible.
• Incorporates proven Suspension Expansion Culture™ (SEC™) Technology available as low serum or serum-free formulations.

Available with a low serum formulation for the following species:

• Human
• Non-human primate (Macaca fascicularis (Cynomologus) and Macaca mulatta (Rhesus))
• Horse
• Pig
• Sheep
• Dog
• Rat
• Mouse

Available with serum-free formulation for the following species:

• Human
• Primate
• Mouse

HemoFLUOR™ is available for the following cell populations:

Stem Cells 

• SC-HPP X: IL-3, IL-6, SCF, Flt3-L (only for human cells and only serum-free)
• SC-HPP 1: IL-3, IL-6, SCF, TPO, Flt3-L
• SC-HPP 2: EPO, GM-CSF, IL-2, IL-3, IL-6, IL-7, SCF, TPO, Flt3-L
• SC-GEMM 1: EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L
• SC-GEMM 2: EPO, GM-CSF, IL-3, IL-6, SCF, TPO
• SC-GEM 1: EPO, GM-CSF, IL-3, IL-6, SCF
• SC-GEM 2: EPO, GM-CSF, IL-3, SCF
• SC-GEM 3: EPO, GM-CSF, G-CSF, IL-3, SCF

Primitive Stem Cell-Producing, T-Lymphocyte Cell Populations

• SC-HPP XT: IL-3, IL-6, SCF, Flt3-L CD3 CD28 (only for human cells and only serum-free; see also T-CFC)
• SC-HPP 1T: IL-3, IL-6, SCF, Flt3-L IL-2, CD3 CD28 (only for human cells and only serum-free; see also T-CFC)

Progenitor Cells

• P-BFU 1: EPO, IL-3, SCF
• P-BFU 2: EPO alone
• P-GM 1: GM-CSF, IL-3, SCF
• P-GM 2: GM-CSF, G-CSF, IL-3, SCF
• P-GM 3: GM-CSF alone
• P-Mk 1: TPO, IL-3, SCF
• P-Mk 2: TPO alone

Lymphopoietic Cells

• P-Tcell 1: IL-2 alone
• P-Tcell 2: CD3 CD28 (human  and mouse cells only)
• P-Tcell 3: IL-2, CD3 CD28 (human and mouse cells only)
• P-Bcell 1: IL-7 only (primate, rat, mouse and canine)
• P-Bcell 2: Cocktail includes cytokines only (human cells only)
• P-Bcell 3: Cocktail includes IgD-Dex  cytokines (human and mouse)
• For mature immune cells, please refer to ImmunoFluor™, ImmunoFluor™ TCP and ImmunoFluor™ BCP

No growth factors: Add growth factors/cytokines of choice.

HemoFLUOR™  "Global" Population Assays
 

4-Population "Global" Assays include reagents to measure:

• SC-GEMM 1
• P-BFU 1
• P-GM 1
• P-Mk 1
• Background controls

5-Population "Global" Assays include reagents to measure:

• SC-HPP 2
• SC-GEMM 1
• P-BFU 1
• P-GM 1
• P-Mk 1
• Background control

7-Population "Global" Assays include reagents to measure:

• SC-HPP 2
• SC-GEMM 1
• P-BFU 1
• P-GM 1
• P-Mk 1
• P-Tcell 3
• P-Bcell 3
• Background control

(1) = The number of samples tested depends on the number of replicates/sample performed. For example, if 4 replicates/sample are performed, then 24 samples can be analyzed on a single 96-well plate. For 6 replicates, 16 samples. For 8 replicates, 12 replicates. These samples, however, do not have to performed on the same day.
(2) = Suspension Expansion Culture™ (SEC™) Technology takes the place of methylcellulose, allowing easier and faster dispensing.
(3) = Some HALO® assays, including STEMpredict™ and all HALO®-Tox HT assays for hematotoxicity testing are available with 384-well plates upon request.
(4) = Multimode plate readers are also av available that have the capability of luminescence, fluorescence and absorbance.
(5) = HemoGLO™ does not include the reagents to calibrate and standardize the assay. However, these reagents are available separately for this assay.
(6) = The total culture volume will depend on the number of replicates required. For example, if 6 replicates are required, a total volume of 0.8mL should be prepared.
(7) = Culture time depends on the state of fractionation or purification of the source cells, cell population tested and species. In some cases it also depends on the assay itself. For example, STEMpredict™ is a 3-day assay.
(8) = Instrument calibration is performed using the ATP controls included with the assay kit or obtained separately for HemoGLO™.
(9) = Standardization implies performing a standard curve. ATP standards are included in all HALO® assay kits and can be obtained separately for HemoGLO™.. 
10 = The inclusion of standards and controls allows the results to be compared to measurement assurance parameters (provided) to ensure the assay is working correctly. This allows proficiency testing to be determined directly prior to measuring samples. 
11 = Measurement assurance parameters are included in all HALO® assays and when standards and controls are purchased separately for HemoGLO™.
12 = A validated assay allows the user to trust the results obtained. This can only be achieved if standards and controls are included. HALO® and HemoGLO™ have been validated. The validation parameters are included for all HALO® assays and included when the optional standardization kit is purchased for HemoGLO™. Standards and controls allow for in-house validation.

For Research Use Only.

A fluorescence plate reader or multimode plate reader with an excitation filter at 530-560nm and an emission filter at 590nm.

• HemoFLUOR™-96 Master Mix (available in low serum and serum-free formulations)
• Resazurin Reagent
• 3% SDS to stop the reaction
• Resorufin to standardize the assay
• Non-sterile 96-well plate use to standardize the assay
• Sterile 96-well culture plates
• Sterile adhesive fo