HemoFLUOR™

The In Vitro Standardized and Validated Bioluminescence Research Assay Platform for Lympho-Hematopoietic Cells

HemoFLUOR™

The Easy and Rapid In Vitro Research Bioluminescence Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

HemoFLUOR™

The Easy and Rapid In Vitro Research Bioluminescence Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

HemoFLUOR™

The Easy and Rapid In Vitro Research Bioluminescence Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

HemoFLUOR™

The Easy and Rapid In Vitro Research Bioluminescence Assay for Measuring Cell Viability and ProlifeDogion of Lympho-Hematopoietic Cells

HemoFLUOR™

The Easy and Rapid In Vitro Research Bioluminescence Assay for Measuring Cell Viability and ProlifeHorseion of Lympho-Hematopoietic Cells

HemoFLUOR™

The Easy and Rapid In Vitro Research Fluorescence Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

HemoFLUOR™

The Easy and Rapid In Vitro Research Bioluminescence Assay for Measuring Cell Viability and ProlifeSheepion of Lympho-Hematopoietic Cells

Please click on the links to take you directly to the videos.

How to Setup HALO®, HemoFLUOR™ or HemoLIGHT™ Hematopoietic Research Assays

How to Perform a Fluorescence-Based Readout (for HemoFLUOR™ Research Assays)

Please navigate to HALO® or HemoLIGHT™ to see how to perform an ATP bioluminescence or absorbance readout. 

• Determine viability, proliferation ability and potential.
• Compare hematopoietic cell populations from different species.
• Replace hematopoietic colony-forming cell (CFC) assay.
• Experimental transplantation models.
• Effects of growth factors/cytokines.
• In vivo to in vitro concordance.
• Gene targeting assays.

• No colony counting needed.
• Non-subjective, instrument-based and quantitative.
• Uses a single-step, resazurin (alamarBlue™) reagent that is converted to a pink/red fluorescent resorufin product (excitation filter, 560nm; emission filter, 590nm).
• Multiplex with flow cytometry allowing cells to be detected using other fluorescent labels/markers. 
• Multiplex with bioluminescence assays.
• Results in about 4-7 days depending on cell population and species.
• Simple, time efficient, cost effective and flexible.
• Incorporates proven Suspension Expansion Culture™ (SEC™) Technology available as low serum or serum-free formulations.

Available with a low serum formulation for the following species:

• Human
• Non-human primate (Macaca fascicularis (Cynomologus) and Macaca mulatta (Rhesus))
• Horse
• Pig
• Sheep
• Dog
• Rat
• Mouse

Available with serum-free formulation for the following species:

• Human
• Primate
• Mouse

HemoFLUOR™ is available for the following cell populations:

Stem Cells 

• SC-HPP X: IL-3, IL-6, SCF, Flt3-L (only for human cells and only serum-free)
• SC-HPP 1: IL-3, IL-6, SCF, TPO, Flt3-L
• SC-HPP 2: EPO, GM-CSF, IL-2, IL-3, IL-6, IL-7, SCF, TPO, Flt3-L
• SC-GEMM 1: EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L
• SC-GEMM 2: EPO, GM-CSF, IL-3, IL-6, SCF, TPO
• SC-GEM 1: EPO, GM-CSF, IL-3, IL-6, SCF
• SC-GEM 2: EPO, GM-CSF, IL-3, SCF
• SC-GEM 3: EPO, GM-CSF, G-CSF, IL-3, SCF

Primitive Stem Cell-Producing, T-Lymphocyte Cell Populations

• SC-HPP XT: IL-3, IL-6, SCF, Flt3-L CD3 CD28 (only for human cells and only serum-free; see also T-CFC)
• SC-HPP 1T: IL-3, IL-6, SCF, Flt3-L IL-2, CD3 CD28 (only for human cells and only serum-free; see also T-CFC)

Progenitor Cells

• P-BFU 1: EPO, IL-3, SCF
• P-BFU 2: EPO alone
• P-GM 1: GM-CSF, IL-3, SCF
• P-GM 2: GM-CSF, G-CSF, IL-3, SCF
• P-GM 3: GM-CSF alone
• P-Mk 1: TPO, IL-3, SCF
• P-Mk 2: TPO alone

Lymphopoietic Cells

• P-Tcell 1: IL-2 alone
• P-Tcell 2: CD3 CD28 (human  and mouse cells only)
• P-Tcell 3: IL-2, CD3 CD28 (human and mouse cells only)
• P-Bcell 1: IL-7 only (primate, rat, mouse and canine)
• P-Bcell 2: Cocktail includes cytokines only (human cells only)
• P-Bcell 3: Cocktail includes IgD-Dex  cytokines (human and mouse)
• For mature immune cells, please refer to ImmunoFluor™, ImmunoFluor™ TCP and ImmunoFluor™ BCP

No growth factors: Add growth factors/cytokines of choice.

HemoFLUOR™  "Global" Population Assays
 

4-Population "Global" Assays include reagents to measure:

• SC-GEMM 1
• P-BFU 1
• P-GM 1
• P-Mk 1
• Background controls

5-Population "Global" Assays include reagents to measure:

• SC-HPP 2
• SC-GEMM 1
• P-BFU 1
• P-GM 1
• P-Mk 1
• Background control

7-Population "Global" Assays include reagents to measure:

• SC-HPP 2
• SC-GEMM 1
• P-BFU 1
• P-GM 1
• P-Mk 1
• P-Tcell 3
• P-Bcell 3
• Background control

• Incorporates the measurement of non-fluorescent Resazurin (alimaBlue™) to fluorescent resorufin in an oxidation/reduction reaction that occurs only in live cells. The amount of fluorescence (relative fluorescence units, RFU) is dependent on the metabolic status of the cells and therefore is not only a measure of metabolic viability, but cell proliferation or inhibition.
• After culture, add 10μL of reagent, mix and measure signal between 30 minutes and 4 hours in a fluorescence plate reader.
• Uses a plate fluorometer or multi mode plate reader to measure fluorescence with an excitation filter 560nm and an emission filter of 590nm.

For Research Use Only.

A fluorescence plate reader or multimode plate reader with an excitation filter at 390-400nm and an emission filter at 505nm.

• HemoFLUOR™-96 Master Mix (available in low serum and serum-free formulations)
• Resazurin Reagent
• 3% SDS to stop the reaction
• Resorufin to standardize the assay
• Non-sterile 96-well plate use to standardize the assay
• Sterile 96-well culture plates
• Sterile adhesive fo