HemoLIGHT™

The Easy and Rapid In Vitro Research Absorbance Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

HemoLIGHT™

The Easy and Rapid In Vitro Research Absorbance Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

HemoLIGHT™

The Easy and Rapid In Vitro Research Absorbance Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

HemoLIGHT™

TThe Easy and Rapid In Vitro Research Absorbance Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

HemoLIGHT™

The Easy and Rapid In Vitro Research Absorbance Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

HemoLIGHT™

TThe Easy and Rapid In Vitro Research Absorbance Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

HemoLIGHT™

The Easy and Rapid In Vitro Research Absorbance Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

HemoLIGHT™

The Easy and Rapid In Vitro Research Absorbance Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

Please click on the links to take you directly to the videos.

How to Setup HALO®, HemoFLUOR™ or HemoLIGHT™ Hematopoietic Research Assays

How to Perform a Absorbance-Based Readout (for HemoLIGHT™ Research Assays)

Please navigate to HALO® or HemoFLUOR™ to see how to perform an ATP bioluminescence or absorbance readout. 

• Determine viability, proliferation ability and potential.
• Compare hematopoietic cell populations from different species.
• Replace hematopoietic colony-forming cell (CFC) assay applications.
• Experimental transplantation models.
• Measure effects of growth factors/cytokines.
• In vivo to in vitro assay concordance.
• Gene targeting assays.

• Uses a colorimetric/absorbance ELISA plate reader, available in most laboratories.
• No colony counting required.
• Non-subjective, instrument-based and quantitative.
• Results in about 7 days depending on species.
• Complete assay kit; includes everything needed to culture and measure hematopoietic cell growth.
• Simple, time efficient and cost effective. 
• Incorporates proven Suspension Expansion Culture™ (SEC™) Technology.

Available with low-serum SEC™ Growth Media for:

• Human
• Non-human primate (Macaca fascicularis (Cynomologus) and Macaca mulatta (Rhesus)
• Horse
• Pig
• Sheep
• Dog
• Rat
• Mouse

Available with serum-free SEC™ Growth Media for:

• Human
• Primate
• Mouse

(See Lympho-Hematopoietic System to view cell populations and growth factor/cytokine cocktails)

HemoLIGHT™-96 Assays:

Stem Cells (download the Stem Cell Population Heirarchy Chart)

• SC-HPP X: IL-3, IL-6, SCF, Flt3-L (only for human cells and only serum-free)
• SC-HPP 1: IL-3, IL-6, SCF, TPO, Flt3-L
• SC-HPP 2: EPO, GM-CSF, IL-2, IL-3, IL-6, IL-7, SCF, TPO, Flt3-L
• SC-GEMM 1: EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L
• SC-GEMM 2: EPO, GM-CSF, IL-3, IL-6, SCF, TPO
• SC-GEM 1: EPO, GM-CSF, IL-3, IL-6, SCF
• SC-GEM 2: EPO, GM-CSF, IL-3, SCF
• SC-GEM 3: EPO, GM-CSF, G-CSF, IL-3, SCF

Primitive Stem Cell-Producing, T-Lymphocyte Cell Populations

• SC-HPP XT: IL-3, IL-6, SCF, Flt3-L CD3 CD28 (only for human cells and only serum-free; see also T-CFC)
• SC-HPP 1T: IL-3, IL-6, SCF, Flt3-L IL-2, CD3 CD28 (only for human cells and only serum-free; see also T-CFC)

Progenitor Cells

• P-BFU 1: EPO, IL-3, SCF
• P-BFU 2: EPO alone
• P-GM 1: GM-CSF, IL-3, SCF
• P-GM 2: GM-CSF, G-CSF, IL-3, SCF
• P-GM 3: GM-CSF alone
• P-Mk 1: TPO, IL-3, SCF
• P-Mk 2: TPO alone

Lymphopoietic cells

• P-Tcell 1: IL-2 alone
• P-Tcell 2: CD3 CD28 (human and mouse cells only)
• P-Tcell 3: IL2, CD3 CD28 (human and mouse cells only)
• P-Bcell 1: IL-7 only (primate, rat, mouse and canine)
• P-Bcell 2: Cocktail includes cytokines only (human cells only)
• P-Bcell 3: Cocktail includes IgD-Dex cytokines (human and mouse)
• For studying mature immune cells, please refer to ImmunoLight™, ImmunoLight™ TCP and ImmunoLight™ BCP

No growth factors: Add growth factors/cytokines of choice.
 

HemoLIGHT™-96 Research "Global" Population Assays
 

4-Population "Global" Assays include reagents to measure:

• SC-GEMM 1
• P-BFU 1
• P-GM 1
• P-Mk 1
• Background controls

5-Population "Global" Assays include reagents to measure:

• SC-HPP 2
• SC-GEMM 1
• P-BFU 1
• P-GM 1
• P-Mk 1
• Background control

7-Population "Global" Assays include reagents to measure:

• SC-HPP 2
• SC-GEMM 1
• P-BFU 1
• P-GM 1
• P-Mk 1
• P-Tcell 3
• P-Bcell 3
• Background control

(1) = The number of samples tested depends on the number of replicates/sample performed. For example, if 4 replicates/sample are performed, then 24 samples can be analyzed on a single 96-well plate. For 6 replicates, 16 samples. For 8 replicates, 12 replicates. These samples, however, do not have to performed on the same day.
(2) = Suspension Expansion Culture™ (SEC™) Technology takes the place of methylcellulose, allowing easier and faster dispensing.
(3) = Some HALO® assays, including STEMpredict™ and all HALO®-Tox HT assays for hematotoxicity testing are available with 384-well plates upon request.
(4) = Multimode plate readers are also av available that have the capability of luminescence, fluorescence and absorbance.
(5) = HemoGLO™ does not include the reagents to calibrate and standardize the assay. However, these reagents are available separately for this assay.
(6) = The total culture volume will depend on the number of replicates required. For example, if 6 replicates are required, a total volume of 0.8mL should be prepared.
(7) = Culture time depends on the state of fractionation or purification of the source cells, cell population tested and species. In some cases it also depends on the assay itself. For example, STEMpredict™ is a 3-day assay.
(8) = Instrument calibration is performed using the ATP controls included with the assay kit or obtained separately for HemoGLO™.
(9) = Standardization implies performing a standard curve. ATP standards are included in all HALO® assay kits and can be obtained separately for HemoGLO™.. 
10 = The inclusion of standards and controls allows the results to be compared to measurement assurance parameters (provided) to ensure the assay is working correctly. This allows proficiency testing to be determined directly prior to measuring samples. 
11 = Measurement assurance parameters are included in all HALO® assays and when standards and controls are purchased separately for HemoGLO™.
12 = A validated assay allows the user to trust the results obtained. This can only be achieved if standards and controls are included. HALO® and HemoGLO™ have been validated. The validation parameters are included for all HALO® assays and included when the optional standardization kit is purchased for HemoGLO™. Standards and controls allow for in-house validation.

• Incorporates a terazolium WST-1 reagent that is reduced to a soluble yellow formazan, which can be measured at an maximum absorbance of 440nm (420nm to 480nm) in an absorbance plate reader.
• After culture, add 10μl of the WST-1 reagent, mix and read absorbance at 440nm after 1-4 hours in a 96-well plate reader. Plates can be returned to incubator and re-read at a later time for increased sensitivity.
• No solubilization step as in a MTT reaction. Replaces MTT assays.

For Research Use Only.

An absorbance plate reader or multimode plate reader to measure absorbance at 440nm.

• HemoLIGHT™ Master Mix (available with low serum and serum-free formulations)
• WST-1 reagent
• Sterile 96-well clear culture plates
• Sterile adhesive foil covers to keep unused wells sterile

Download the HemoLIGHT™ Technical Manual