HemoLIGHT™

The Easy and Rapid In Vitro Research Absorbance Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

HemoLIGHT™

The Easy and Rapid In Vitro Research Absorbance Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

HemoLIGHT™

The Easy and Rapid In Vitro Research Absorbance Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

HemoLIGHT™

TThe Easy and Rapid In Vitro Research Absorbance Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

HemoLIGHT™

The Easy and Rapid In Vitro Research Absorbance Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

HemoLIGHT™

TThe Easy and Rapid In Vitro Research Absorbance Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

HemoLIGHT™

The Easy and Rapid In Vitro Research Absorbance Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

HemoLIGHT™

The Easy and Rapid In Vitro Research Absorbance Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells

Please click on the links to take you directly to the videos.

How to Setup HALO®, HemoFLUOR™ or HemoLIGHT™ Hematopoietic Research Assays

How to Perform a Absorbance-Based Readout (for HemoLIGHT™ Research Assays)

Please navigate to HALO® or HemoFLUOR™ to see how to perform an ATP bioluminescence or absorbance readout. 

• Determine viability, proliferation ability and potential.
• Compare hematopoietic cell populations from different species.
• Replace hematopoietic colony-forming cell (CFC) assay applications.
• Experimental transplantation models.
• Measure effects of growth factors/cytokines.
• In vivo to in vitro assay concordance.
• Gene targeting assays.

• Uses a colorimetric/absorbance ELISA plate reader, available in most laboratories.
• No colony counting required.
• Non-subjective, instrument-based and quantitative.
• Results in about 7 days depending on species.
• Complete assay kit; includes everything needed to culture and measure hematopoietic cell growth.
• Simple, time efficient and cost effective. 
• Incorporates proven Suspension Expansion Culture™ (SEC™) Technology.

Available with low-serum SEC™ Growth Media for:

• Human
• Non-human primate (Macaca fascicularis (Cynomologus) and Macaca mulatta (Rhesus)
• Horse
• Pig
• Sheep
• Dog
• Rat
• Mouse

Available with serum-free SEC™ Growth Media for:

• Human
• Primate
• Mouse

(See Lympho-Hematopoietic System to view cell populations and growth factor/cytokine cocktails)

HemoLIGHT™-96 Assays:

Stem Cells (download the Stem Cell Population Heirarchy Chart)

• SC-HPP X: IL-3, IL-6, SCF, Flt3-L (only for human cells and only serum-free)
• SC-HPP 1: IL-3, IL-6, SCF, TPO, Flt3-L
• SC-HPP 2: EPO, GM-CSF, IL-2, IL-3, IL-6, IL-7, SCF, TPO, Flt3-L
• SC-GEMM 1: EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L
• SC-GEMM 2: EPO, GM-CSF, IL-3, IL-6, SCF, TPO
• SC-GEM 1: EPO, GM-CSF, IL-3, IL-6, SCF
• SC-GEM 2: EPO, GM-CSF, IL-3, SCF
• SC-GEM 3: EPO, GM-CSF, G-CSF, IL-3, SCF

Primitive Stem Cell-Producing, T-Lymphocyte Cell Populations

• SC-HPP XT: IL-3, IL-6, SCF, Flt3-L CD3 CD28 (only for human cells and only serum-free; see also T-CFC)
• SC-HPP 1T: IL-3, IL-6, SCF, Flt3-L IL-2, CD3 CD28 (only for human cells and only serum-free; see also T-CFC)

Progenitor Cells

• P-BFU 1: EPO, IL-3, SCF
• P-BFU 2: EPO alone
• P-GM 1: GM-CSF, IL-3, SCF
• P-GM 2: GM-CSF, G-CSF, IL-3, SCF
• P-GM 3: GM-CSF alone
• P-Mk 1: TPO, IL-3, SCF
• P-Mk 2: TPO alone

Lymphopoietic cells

• P-Tcell 1: IL-2 alone
• P-Tcell 2: CD3 CD28 (human and mouse cells only)
• P-Tcell 3: IL2, CD3 CD28 (human and mouse cells only)
• P-Bcell 1: IL-7 only (primate, rat, mouse and canine)
• P-Bcell 2: Cocktail includes cytokines only (human cells only)
• P-Bcell 3: Cocktail includes IgD-Dex cytokines (human and mouse)
• For studying mature immune cells, please refer to ImmunoLight™, ImmunoLight™ TCP and ImmunoLight™ BCP

No growth factors: Add growth factors/cytokines of choice.
 

HemoLIGHT™-96 Research "Global" Population Assays
 

4-Population "Global" Assays include reagents to measure:

• SC-GEMM 1
• P-BFU 1
• P-GM 1
• P-Mk 1
• Background controls

5-Population "Global" Assays include reagents to measure:

• SC-HPP 2
• SC-GEMM 1
• P-BFU 1
• P-GM 1
• P-Mk 1
• Background control

7-Population "Global" Assays include reagents to measure:

• SC-HPP 2
• SC-GEMM 1
• P-BFU 1
• P-GM 1
• P-Mk 1
• P-Tcell 3
• P-Bcell 3
• Background control

• Incorporates a terazolium WST-1 reagent that is reduced to a soluble yellow formazan, which can be measured at an maximum absorbance of 440nm (420nm to 480nm) in an absorbance plate reader.
• After culture, add 10μl of the WST-1 reagent, mix and read absorbance at 440nm after 1-4 hours in a 96-well plate reader. Plates can be returned to incubator and re-read at a later time for increased sensitivity.
• No solubilization step as in a MTT reaction. Replaces MTT assays.

For Research Use Only.

An absorbance plate reader or multimode plate reader to measure absorbance at 440nm.

• HemoLIGHT™ Master Mix (available with low serum and serum-free formulations)
• WST-1 reagent
• Sterile 96-well clear culture plates
• Sterile adhesive foil covers to keep unused wells sterile

HemoLIGHT™ Assay Manual