HALO®
(Hematopoietic Assays via Luminescence Output)
An In Vitro Standardized and Validated
Bioluminescence Research Assay for
Lympho-Hematopoietic Cells
Buy HALO™ for Human Cells
HALO™
The In Vitro Standardized and Validated Bioluminescence Research Assay Platform for Lympho-Hematopoietic Cells
| Cell Population | Species | Formulation | Catalog Number | Quantity | |
|---|---|---|---|---|---|
| Stem Cells | |||||
| SC-HPP 1 | Human | Low serum. Methylcellulose-free | K2-HPP1-1H | 1 Kit | |
| SC-HPP 2 | Human | Low serum. Methylcellulose-free | K2-HPP2-1H | 1 Kit | |
| SC-GEMM 1 | Human | Low serum. Methylcellulose-free | K2-GEMM1-1H | 1 Kit | |
| SC-GEMM 2 | Human | Low serum. Methylcellulose-free | K2-GEMM2-1H | 1 Kit | |
| SC-GEMM 3 | Human | Low serum. Methylcellulose-free | K2-GEMM3-1H | 1 Kit | |
| SC-GEM 1 | Human | Low serum. Methylcellulose-free | K2-GEM1-1H | 1 Kit | |
| SC-GEM 3 | Human | Low serum. Methylcellulose-free | K2-GEM3-1H | 1 Kit | |
| SC-GEM 2 | Human | Low serum. Methylcellulose-free | K2-GEM2-1H | 1 Kit | |
| Stem Cells | |||||
| SC-HPP1T | Human | Serum-free. Methylcellulose-free | K2SF-HPP1T-1H | 1 Kit | |
| SC-HPPX | Human | Serum-free. Methylcellulose-free | K2SF-HPPX-1H | 1 Kit | |
| SC-HPPXT | Human | Serum-free. Methylcellulose-free | K2SF-HPPXT-1H | 1 Kit | |
| SC-HPP 1 | Human | Serum-free. Methylcellulose-free | K2SF-HPP1-1H | 1 Kit | |
| SC-HPP 2 | Human | Serum-free. Methylcellulose-free | K2SF-HPP2-1H | 1 Kit | |
| SC-GEMM 1 | Human | Serum-free. Methylcellulose-free | K2SF-GEMM1-1H | 1 Kit | |
| SC-GEMM 2 | Human | Serum-free. Methylcellulose-free | K2SF-GEMM2-1H | 1 Kit | |
| SC-GEMM 3 | Human | Serum-free. Methylcellulose-free | K2SF-GEMM3-1H | 1 Kit | |
| SC-GEM 1 | Human | Serum-free. Methylcellulose-free | K2SF-GEM1-1H | 1 Kit | |
| SC-GEM 2 | Human | Serum-free. Methylcellulose-free | K2SF-GEM2-1H | 1 Kit | |
| SC-GEM 3 | Human | Serum-free. Methylcellulose-free | K2SF-GEM3-1H | 1 Kit | |
| Hematopoietic Progenitor Cells | |||||
| P-BFU 1 | Human | Low serum. Methylcellulose-free | K2-BFU1-1H | 1 Kit | |
| P-BFU 2 | Human | Low serum. Methylcellulose-free | K2-BFU2-1H | 1 Kit | |
| P-GM 1 | Human | Low serum. Methylcellulose-free | K2-GM1-1H | 1 Kit | |
| P-GM 2 | Human | Low serum. Methylcellulose-free | K2-GM2-1H | 1 Kit | |
| P-GM 3 | Human | Low serum. Methylcellulose-free | K2-GM3-1H | 1 Kit | |
| P-Mk 1 | Human | Low serum. Methylcellulose-free | K2-Mk1-1H | 1 Kit | |
| Hematopoietic Progenitor Cells | |||||
| P-BFU 1 | Human | Serum-free. Methylcellulose-free | K2SF-BFU1-1H | 1 Kit | |
| P-BFU 2 | Human | Serum-free. Methylcellulose-free | K2SF-BFU2-1H | 1 Kit | |
| P-GM 1 | Human | Serum-free. Methylcellulose-free | K2SF-GM1-1H | 1 Kit | |
| P-GM 2 | Human | Serum-free. Methylcellulose-free | K2SF-GM2-1H | 1 Kit | |
| P-GM 3 | Human | Serum-free. Methylcellulose-free | K2SF-GM3-1H | 1 Kit | |
| P-Mk 1 | Human | Serum-free. Methylcellulose-free | K2SF-Mk1-1H | 1 Kit | |
| Lymphopoietic Progenitor Cells | |||||
| P-Tcell 1 | Human | Low serum. Methylcellulose-free | K2-T1-1H | 1 Kit | |
| P-Tcell 2 | Human | Low serum. Methylcellulose-free | K2-T2-1H | 1 Kit | |
| P-Tcell 3 | Human | Low serum. Methylcellulose-free | K2-T3-1H | 1 Kit | |
| P-Bcell 2 | Human | Low serum. Methylcellulose-free | K2-B2-1H | 1 Kit | |
| P-Bcell 3 | Human | Low serum. Methylcellulose-free | K2-B3-1H | 1 Kit | |
| Lymphopoietic Progenitor Cells | |||||
| P-Tcell 1 | Human | Serum-free. Methylcellulose-free | K2SF-T1-1H | 1 Kit | |
| P-Tcell 2 | Human | Serum-free. Methylcellulose-free | K2SF-T2-1H | 1 Kit | |
| P-Tcell 3 | Human | Serum-free. Methylcellulose-free | K2SF-T3-1H | 1 Kit | |
| No Growth Factors Added | |||||
| Any | Human | Low serum. Methylcellulose-free | K2-0-1H | 1 Kit | |
| Any | Human | Serum-free. Methylcellulose-free | K2SF-0-1H | 1 Kit | |
| "Global" Research Assays | |||||
| "Global" 4-Pop Research | Human | Low serum, methylcellulose-free | K2-4P-5H | 1 Kit | |
| "Global" 5-Pop Research | Human | Low serum, methylcellulose-free | K2-5P-6H | 1 Kit | |
| "Global" 7-Pop Research | Human | Low serum, methylcellulose-free | K2-7P-8H | 1 Kit | |
| "Global" Research Assays | |||||
| "Global" 4-Pop Research | Human | Serum-free. Methylcellulose-free | K2SF-4P-5H | 1 Kit | |
| "Global" 5-Pop Research | Human | Serum-free. Methylcellulose-free | K2SF-5P-6H | 1 Kit | |
| "Global" 7-Pop Research | Human | Serum-free. Methylcellulose-free | K2SF-7P-8H | 1 Kit | |
HALO™
The Easy and Rapid In Vitro Research Bioluminescence Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells
| Cell Population | Species | Formulation | Catalog Number | Quantity | |
|---|---|---|---|---|---|
| Stem Cells | |||||
| SC-HPP 2 | Mouse | Low serum. Methylcellulose-free | K2-HPP2-1M | 1 Kit | |
| SC-GEMM 1 | Mouse | Low serum. Methylcellulose-free | K2-GEMM1-1M | 1 Kit | |
| Stem Cells | |||||
| SC-HPP 2 | Mouse | Serum-free. Methylcellulose-free | K2SF-HPP2-1M | 1 Kit | |
| SC-GEMM 1 | Mouse | Serum-free. Methylcellulose-free | K2SF-GEMM1-1M | 1 Kit | |
| Hematopoietic Progenitor Cells | |||||
| P-BFU 1 | Mouse | Low serum. Methylcellulose-free | K2-BFU1-1M | 1 Kit | |
| P-BFU 2 | Mouse | Low serum. Methylcellulose-free | K2-BFU2-1M | 1 Kit | |
| P-GM 1 | Mouse | Low serum. Methylcellulose-free | K2-GM1-1M | 1 Kit | |
| P-GM 3 | Mouse | Low serum. Methylcellulose-free | K2-GM3-1M | 1 Kit | |
| P-Mk 1 | Mouse | Low serum. Methylcellulose-free | K2-Mk1-1M | 1 Kit | |
| Hematopoietic Progenitor Cells | |||||
| P-BFU 1 | Mouse | Serum-free. Methylcellulose-free | K2SF-BFU1-1M | 1 Kit | |
| P-BFU 2 | Mouse | Serum-free. Methylcellulose-free | K2SF-BFU2-1M | 1 Kit | |
| P-GM 1 | Mouse | Serum-free. Methylcellulose-free | K2SF-GM1-1M | 1 Kit | |
| P-GM 3 | Mouse | Serum-free. Methylcellulose-free | K2SF-GM3-1M | 1 Kit | |
| P-Mk 1 | Mouse | Serum-free. Methylcellulose-free | K2SF-Mk1-1M | 1 Kit | |
| Lymphopoietic Progenitor Cells | |||||
| P-Tcell 1 | Mouse | Low serum. Methylcellulose-free | K2-T1-1M | 1 Kit | |
| P-Bcell 3 | Mouse | Low serum. Methylcellulose-free | K2-B3-1M | 1 Kit | |
| Lymphopoietic Progenitor Cells | |||||
| P-Tcell 1 | Mouse | Serum-free. Methylcellulose-free | K2SF-T1-1M | 1 Kit | |
| No Growth Factors Added | |||||
| Any | Mouse | Low serum. Methylcellulose-free | K2-0-1M | 1 Kit | |
| Any | Mouse | Serum-free. Methylcellulose-free | K2SF-0-1M | 1 Kit | |
| "Global" Research Assays | |||||
| "Global" 4-Pop Research | Mouse | Low serum, methylcellulose-free | K2-4P-5M | 1 Kit | |
| "Global" 5-Pop Research | Mouse | Low serum, methylcellulose-free | K2-5P-6M | 1 Kit | |
| "Global" 7-Pop Research | Mouse | Low serum, methylcellulose-free | K2-7P-8M | 1 Kit | |
| "Global" Research Assays | |||||
| "Global" 4-Pop Research | Mouse | Serum-free. Methylcellulose-free | K2SF-4P-5M | 1 Kit | |
| "Global" 5-Pop Research | Mouse | Serum-free. Methylcellulose-free | K2SF-5P-6M | 1 Kit | |
| "Global" 7-Pop Research | Mouse | Serum-free. Methylcellulose-free | K2SF-7P-8M | 1 Kit | |
HALO™
The Easy and Rapid In Vitro Research Bioluminescence Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells
| Cell Population | Species | Formulation | Catalog Number | Quantity | |
|---|---|---|---|---|---|
| Stem Cells | |||||
| SC-HPP 2 | Primate | Low serum. Methylcellulose-free | K2-HPP2-1Pr | 1 Kit | |
| SC-GEMM 1 | Primate | Low serum. Methylcellulose-free | K2-GEMM1-1Pr | 1 Kit | |
| Stem Cells | |||||
| SC-HPP 2 | Primate | Serum-free. Methylcellulose-free | K2SF-HPP2-1Pr | 1 Kit | |
| SC-GEMM 1 | Primate | Serum-free. Methylcellulose-free | K2SF-GEMM1-1Pr | 1 Kit | |
| Hematopoietic Progenitor Cells | |||||
| P-BFU 1 | Primate | Low serum. Methylcellulose-free | K2-BFU1-1Pr | 1 Kit | |
| P-BFU 2 | Primate | Low serum. Methylcellulose-free | K2-BFU2-1Pr | 1 Kit | |
| P-GM 1 | Primate | Low serum. Methylcellulose-free | K2-GM1-1Pr | 1 Kit | |
| P-GM 3 | Primate | Low serum. Methylcellulose-free | K2-GM3-1Pr | 1 Kit | |
| P-Mk 1 | Primate | Low serum. Methylcellulose-free | K2-Mk1-1Pr | 1 Kit | |
| Hematopoietic Progenitor Cells | |||||
| P-BFU 1 | Primate | Serum-free. Methylcellulose-free | K2SF-BFU1-1Pr | 1 Kit | |
| P-BFU 2 | Primate | Serum-free. Methylcellulose-free | K2SF-BFU2-1Pr | 1 Kit | |
| P-GM 1 | Primate | Serum-free. Methylcellulose-free | K2SF-GM1-1Pr | 1 Kit | |
| P-GM 3 | Primate | Serum-free. Methylcellulose-free | K2SF-GM3-1Pr | 1 Kit | |
| P-Mk 1 | Primate | Serum-free. Methylcellulose-free | K2SF-Mk1-1Pr | 1 Kit | |
| Lymphopoietic Progenitor Cells | |||||
| P-Tcell 1 | Primate | Low serum. Methylcellulose-free | K2-T1-1Pr | 1 Kit | |
| Lymphopoietic Progenitor Cells | |||||
| P-Tcell 1 | Primate | Serum-free. Methylcellulose-free | K2SF-T1-1Pr | 1 Kit | |
| No Growth Factors Added | |||||
| Any | Primate | Low serum. Methylcellulose-free | K2-0-1Pr | 1 Kit | |
| Any | Primate | Serum-free. Methylcellulose-free | K2SF-0-1Pr | 1 Kit | |
| "Global" Research Assays | |||||
| "Global" 4-Pop Research | Primate | Low serum, methylcellulose-free | K2-4P-5Pr | 1 Kit | |
| "Global" 5-Pop Research | Primate | Low serum, methylcellulose-free | K2-5P-6Pr | 1 Kit | |
| "Global" 7-Pop Research | Primate | Low serum, methylcellulose-free | K2-7P-8Pr | 1 Kit | |
| "Global" Research Assays | |||||
| "Global" 4-Pop Research | Primate | Serum-free. Methylcellulose-free | K2SF-4P-5Pr | 1 Kit | |
| "Global" 5-Pop Research | Primate | Serum-free. Methylcellulose-free | K2SF-5P-6Pr | 1 Kit | |
| "Global" 7-Pop Research | Primate | Serum-free. Methylcellulose-free | K2SF-7P-8Pr | 1 Kit | |
HALO™
The Easy and Rapid In Vitro Research Bioluminescence Assay for Measuring Cell Viability and Proliferation of Lympho-Hematopoietic Cells
| Cell Population | Species | Formulation | Catalog Number | Quantity | |
|---|---|---|---|---|---|
| Stem Cells | |||||
| SC-HPP 2 | Rat | Low serum. Methylcellulose-free | K2-HPP2-1R | 1 Kit | |
| SC-GEMM 1 | Rat | Low serum. Methylcellulose-free | K2-GEMM1-1R | 1 Kit | |
| Hematopoietic Progenitor Cells | |||||
| P-BFU 1 | Rat | Low serum. Methylcellulose-free | K2-BFU1-1R | 1 Kit | |
| P-BFU 2 | Rat | Low serum. Methylcellulose-free | K2-BFU2-1R | 1 Kit | |
| P-GM 1 | Rat | Low serum. Methylcellulose-free | K2-GM1-1R | 1 Kit | |
| P-GM 3 | Rat | Low serum. Methylcellulose-free | K2-GM3-1R | 1 Kit | |
| P-Mk 1 | Rat | Low serum. Methylcellulose-free | K2-Mk1-1R | 1 Kit | |
| Lymphopoietic Progenitor Cells | |||||
| P-Tcell 1 | Rat | Low serum. Methylcellulose-free | K2-T1-1R | 1 Kit | |
| P-Bcell 1 | Rat | Low serum. Methylcellulose-free | K2-B1-1R | 1 Kit | |
| No Growth Factors Added | |||||
| Any | Rat | Low serum. Methylcellulose-free | K2-0-1R | 1 Kit | |
| "Global" Research Assays | |||||
| "Global" 4-Pop Research | Rat | Low serum, methylcellulose-free | K2-4P-5R | 1 Kit | |
| "Global" 5-Pop Research | Rat | Low serum, methylcellulose-free | K2-5P-6R | 1 Kit | |
| "Global" 7-Pop Research | Rat | Low serum, methylcellulose-free | K2-7P-8R | 1 Kit | |
HALO™
The Easy and Rapid In Vitro Research Bioluminescence Assay for Measuring Cell Viability and ProlifeDogion of Lympho-Hematopoietic Cells
| Cell Population | Species | Formulation | Catalog Number | Quantity | |
|---|---|---|---|---|---|
| Stem Cells | |||||
| SC-HPP 2 | Dog | Low serum. Methylcellulose-free | K2-HPP2-1C | 1 Kit | |
| SC-GEMM 1 | Dog | Low serum. Methylcellulose-free | K2-GEMM1-1C | 1 Kit | |
| Hematopoietic Progenitor Cells | |||||
| P-BFU 1 | Dog | Low serum. Methylcellulose-free | K2-BFU1-1C | 1 Kit | |
| P-BFU 2 | Dog | Low serum. Methylcellulose-free | K2-BFU2-1C | 1 Kit | |
| P-GM 1 | Dog | Low serum. Methylcellulose-free | K2-GM1-1C | 1 Kit | |
| P-GM 3 | Dog | Low serum. Methylcellulose-free | K2-GM3-1C | 1 Kit | |
| P-Mk 1 | Dog | Low serum. Methylcellulose-free | K2-Mk1-1C | 1 Kit | |
| No Growth Factors Added | |||||
| Any | Dog | Low serum. Methylcellulose-free | K2-0-1C | 1 Kit | |
| "Global" Research Assays | |||||
| "Global" 4-Pop Research | Dog | Low serum, methylcellulose-free | K2-4P-5C | 1 Kit | |
| "Global" 5-Pop Research | Dog | Low serum, methylcellulose-free | K2-5P-6C | 1 Kit | |
| "Global" 7-Pop Research | Dog | Low serum, methylcellulose-free | K2-7P-8C | 1 Kit | |
HALO™
The Easy and Rapid In Vitro Research Bioluminescence Assay for Measuring Cell Viability and ProlifeHorseion of Lympho-Hematopoietic Cells
| Cell Population | Species | Formulation | Catalog Number | Quantity | |
|---|---|---|---|---|---|
| Stem Cells | |||||
| SC-HPP 2 | Horse | Low serum. Methylcellulose-free | K2-HPP2-1E | 1 Kit | |
| SC-GEMM 1 | Horse | Low serum. Methylcellulose-free | K2-GEMM1-1E | 1 Kit | |
| Hematopoietic Progenitor Cells | |||||
| P-BFU 1 | Horse | Low serum. Methylcellulose-free | K2-BFU1-1E | 1 Kit | |
| P-BFU 2 | Horse | Low serum. Methylcellulose-free | K2-BFU2-1E | 1 Kit | |
| P-GM 1 | Horse | Low serum. Methylcellulose-free | K2-GM1-1E | 1 Kit | |
| P-GM 3 | Horse | Low serum. Methylcellulose-free | K2-GM3-1E | 1 Kit | |
| P-Mk 1 | Horse | Low serum. Methylcellulose-free | K2-Mk1-1E | 1 Kit | |
| No Growth Factors Added | |||||
| Any | Horse | Low serum. Methylcellulose-free | K2-0-1E | 1 Kit | |
HALO™
The Easy and Rapid In Vitro Research Bioluminescence Assay for Measuring Cell Viability and ProlifePigion of Lympho-Hematopoietic Cells
| Cell Population | Species | Formulation | Catalog Number | Quantity | |
|---|---|---|---|---|---|
| Stem Cells | |||||
| SC-HPP 2 | Pig | Low serum. Methylcellulose-free | K2-HPP2-1Po | 1 Kit | |
| SC-GEMM 1 | Pig | Low serum. Methylcellulose-free | K2-GEMM1-1Po | 1 Kit | |
| Hematopoietic Progenitor Cells | |||||
| P-BFU 1 | Pig | Low serum. Methylcellulose-free | K2-BFU1-1Po | 1 Kit | |
| P-BFU 2 | Pig | Low serum. Methylcellulose-free | K2-BFU2-1Po | 1 Kit | |
| P-GM 1 | Pig | Low serum. Methylcellulose-free | K2-GM1-1Po | 1 Kit | |
| P-GM 3 | Pig | Low serum. Methylcellulose-free | K2-GM3-1Po | 1 Kit | |
| P-Mk 1 | Pig | Low serum. Methylcellulose-free | K2-Mk1-1Po | 1 Kit | |
| No Growth Factors Added | |||||
| Any | Pig | Low serum. Methylcellulose-free | K2-0-1Po | 1 Kit | |
HALO™
The Easy and Rapid In Vitro Research Bioluminescence Assay for Measuring Cell Viability and ProlifeSheepion of Lympho-Hematopoietic Cells
| Cell Population | Species | Formulation | Catalog Number | Quantity | |
|---|---|---|---|---|---|
| Stem Cells | |||||
| SC-HPP 2 | Sheep | Low serum. Methylcellulose-free | K2-HPP2-1O | 1 Kit | |
| SC-GEMM 1 | Sheep | Low serum. Methylcellulose-free | K2-GEMM1-1O | 1 Kit | |
| Hematopoietic Progenitor Cells | |||||
| P-BFU 1 | Sheep | Low serum. Methylcellulose-free | K2-BFU1-1O | 1 Kit | |
| P-BFU 2 | Sheep | Low serum. Methylcellulose-free | K2-BFU2-1O | 1 Kit | |
| P-GM 1 | Sheep | Low serum. Methylcellulose-free | K2-GM1-1O | 1 Kit | |
| P-GM 3 | Sheep | Low serum. Methylcellulose-free | K2-GM3-1O | 1 Kit | |
| P-Mk 1 | Sheep | Low serum. Methylcellulose-free | K2-Mk1-1O | 1 Kit | |
| No Growth Factors Added | |||||
| Any | Sheep | Low serum. Methylcellulose-free | K2-0-1O | 1 Kit | |
Instructional Videos on How to Setup and Use HALO®
Please click on the links to take you directly to the videos.
How to Setup HALO®, HemoFLUOR™ or HemoLIGHT™ Hematopoietic Research Assays
How to Calibrate and Standardize Any ATP Bioluminescence Assay - Part 1 (for HALO® Research Assays)
How to Calibrate and Standardize Any ATP Bioluminescence Assay - Part 2 (for HALO® Research Assays)
Please navigate to HemoFLUOR™ or HemoLIGHT™ to see how to perform a fluorescence or absorbance readout.
- Measure lympho-hematopoietic clonal proliferation and cell growth.
- Detect and measure proliferation potential and ability of human and animal stem and progenitor cells, including T-cell progenitor cells.
- Reliably and reproducibly replaces all applications of the colony-forming assay (CFU/CFC).
- Measure and compare proliferation status or potential between tissues and cell populations.
- Experimental transplantation models.
- Effects of growth factors and/or cytokines.
- In vivo to in vitro assays.
- Gene targeting assays, e.g. CRISPR-Cas9.
- HALO® measures the viability, proliferation ability and potential of lympho-hematopoietic cells by using a highly sensitive ATP bioluminescence readout.
- Correlates directly with any methylcellulose CFC/CFU, fluorescence or absorbance assay.
- Minimal training, simple (no colony counting required), time efficient and cost effective.
- Results are non-subjective, instrument-based and quantitative.
- Fully standardized using external standards and controls included in assay kit.
- Direct comparison of results between samples, and over time without the normalization of data.
- Results in 4 days for animal cells, and 5 days for human cells.
- Incorporates proprietary Suspension Expansion Culture™ (SEC™) Growth Medium to distinguish up to 7 different stem cell, and 9 different progenitor cell populations from 8 species.
- Also available as 4-, 5- and 7-Population "Global" assays using low serum or serum-free SEC™ growth media.
- HALO® can be multiplexed with other assay readouts such as flow cytometric phenotypic analysis or gene expression assays.
Please visit the Instructional Videos page
An example of an ATP standard curve and control results are shown in the diagram below and actual values for the kit being used are given in the Technical Manual. If the values obtained are within the ranges given in the Technical Manual, the user can process the samples knowing that the results will be trustworthy.
Species
All ATP bioluminescence assays kits from Preferred Cell Systems™ include an ATP standard(s) and controls. Performing the ATP standard curve and controls is highly recommended. The controls are used to calibrate the instrument and the ATP standard is used to standardize the assay. The values obtained provide the measurement assurance parameters that allow you, the user, to ensure that the results obtained can be trusted.
An example of an ATP standard curve and control results are shown in the diagram below and actual values for the kit being used are given in the Technical Manual. If the values obtained are within the ranges given in the Technical Manual, the user can process the samples knowing that the results will be trustworthy.
HALO® is available with a low serum formulation of SEC™ Growth Media for the following species:
- Human
- Non-human primate (Macaca fascicularis (Cynomologus) and Macaca mulatta (Rhesus))
- Horse
- Pig
- Minipig
- Sheep
- Dog
- Rat
- Mouse
HALO® is also available with serum-free SEC™ Growth Media for:
- Human
- Primate
- Mouse
HALO® is available for the following cell populations:
Stem Cells
- SC-HPP X: IL-3, IL-6, SCF, Flt3-L (only for human cells and only serum-free)
- SC-HPP 1: IL-3, IL-6, SCF, TPO, Flt3-L
- SC-HPP 2: EPO, GM-CSF, IL-2, IL-3, IL-6, IL-7, SCF, TPO, Flt3-L
- SC-GEMM 1: EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L
- SC-GEMM 2: EPO, GM-CSF, IL-3, IL-6, SCF, TPO
- SC-GEM 1: EPO, GM-CSF, IL-3, IL-6, SCF
- SC-GEM 2: EPO, GM-CSF, IL-3, SCF
- SC-GEM 3: EPO, GM-CSF, G-CSF, IL-3, SCF
Primitive Stem Cell-Producing, T-Lymphocyte Cell Populations
- SC-HPP XT: IL-3, IL-6, SCF, Flt3-L CD3 CD28 (only for human cells and only serum-free; see also P-Tcells)
- SC-HPP 1T: IL-3, IL-6, SCF, Flt3-L IL-2, CD3 CD28 (only for human cells and only serum-free; see also P-Tcells)
Progenitor Cells (P)
- P-BFU 1: EPO, IL-3, SCF
- P-BFU 2: EPO alone
- P-GM 1: GM-CSF, IL-3, SCF
- P-GM 3: GM-CSF alone
- P-Mk 1: TPO, IL-3, SCF
Lymphopoietic Cells
- P-Tcell 1: IL-2 alone
- P-Tcell 2: CD3 C28 (human and mouse cells only)
- P-Tcell 3: IL-2, CD3 CD28 (human and mouse cells only)
- P-Bcell 1: Il-7 only (primate, rat, mouse and canine)
- P-Bcell 2: Cocktail includes cytokines only (human cells only)
- P-Bcell 3: Cocktail includes IgD-Dex cytokines (human and mouse)
- For studying mature immune cells, please refer to ImmunoGlo™, ImmunoGlo™ TCP and ImmunoGlo™ BCP
No growth factors: Add growth factors/cytokines of choice.
HALO® "Global" Population Assays
4-Population "Global" Assays include reagents to measure:
- SC-GEMM 1
- P-BFU 1
- P-GM 1
- P-Mk 1
- Background controls
5-Population "Global" Assays include reagents to measure:
- SC-HPP 2
- SC-GEMM 1
- P-BFU 1
- P-GM 1
- P-Mk 1
- Background control
7-Population "Global" Assays include reagents to measure:
- SC-HPP 2
- SC-GEMM 1
- P-BFU 1
- P-GM 1
- P-Mk 1
- P-Tcell 3
- P-Bcell 3
- Background control
Click image for larger view
When You Use HALO®, No Colony Counting is Necessary
Since HALO® does not use methylcellulose, no colonies are produced. Instead, HALO® incorporates Suspension Expansion Culture™ (SEC™) Technology; cells are cultured in suspension. This means it is easier, faster and more accurate to setup HALO® cultures because normal pipettes are used. The question always asked is: If no colonies are produced, how do I know whether the cells have differentiated and how do I detect cell differentiation? The answer lies in flow cytometry. The type of cells produced is dependent on the growth factor cocktail used, just as it is in a normal CFU assay. The diagram above show this. If a SC-GEMM culture is setup in HALO®, you would expect to see the presence of stem cells, erythroid cells, granulocytes and macrophages and megakaryocytes, because that is the definition of the SC-GEMM stem cell population. If the cells are removed from a SC-GEMM culture and analyzed by flow cytometry, these are precisely the cell types that are present in the culture as seen by the blue columns. Similarly, if SC-BFU cultures are setup, you would expect to see a prevalence of CD235a+ (glycophorin-A) cells and that is exactly what you find, together with some granulocytes and macrophages as well as megakaryocytes. In other words, the cells you detect in HALO® cultures by flow cytometry are the cells you would expect for the growth factor cocktail used. Therefore, waiting for differentiation to occur and enumerating different types of colonies is unnecessary when using HALO®.
Click image for larger view
Measuring Proliferation Potential
Proliferation potential is measured by performing a cell dose response and calculating the slope of the linear regression. The steeper the slope, the greater the proliferation potential of the cell population. The greater the proliferation potential, the more primitive the cell population.
Click image for larger view
Example of a 7-Population Comparison Between Human Tissues
This graph shows the response of 7 lympho-hematopoietic cell populations derived from mobilized peripheral blood CD34 cells, bone marrow and umbilical cord blood mononuclear cells. Please note that whereas mobilized peripheral blood CD34 cells are shown at 500 CD34 /well, bone marrow and umbilical cord blood are shown at 5,000 mononuclear cells/well.
Click image for larger view
Comparison of 7-Population Response between Species
The graph shows the response of 7 lympho-hematopoietic populations, SC-HPP 2, SC-GEMM 1, P-BFU 1, P-GM 1, P-Mk 1, P-Tcell, P-Bcell and background, from sheep, pig, dog, rat and mouse bone marrow.
Click image for larger view
Production of T-Lymphocytes from Primitive Lympho-Hematopoietic Stem Cells
Human bone marrow High Proliferative Potential-Stem and Progenitor Cells (SC-HPP) were stimulated with various factors and lymphocyte co-factors to induce the cells out of quiecence and into the T-cell lineage. Such studies can be combined with low cytometric phenotypic analysis to determine different T-cell populations.
For Research Use Only. Not for clinical diagnostic use.
Luminescence plate reader or multimode plate reader with "glow" luminescence measuring capability.
- HALO® Master Mix. Please choose between low serum or serum-free formulations.
- ATP standard
- ATP high and low controls (not included with research kits)
- ATP Enumeration Reagent
- Sterile, 96-well plates
- Non-sterile, 96-well plates
- Sterile, adhesive foil covers
Please note that the same protocol is used for HALO® "Global" 4-, 5- and 7-Population Assay Kits
Please visit the Instructional Videos page
Download the HALO® Technical Manual
Download the ATP Optimization Kit Protocol for First-Time Users
Download Luminometer Setup and RLU to ATP Conversion
Download Certificate of Analysis (CoAs) for ATP Enumeration Reagent (ATP-ER)
Download Certificate of Analysis for ATP Stanadrds
Download Certificate of Analysis for ATP Controls
Download Certificate of Analysis of ATP Reconstitution Reagent
Download Certificate of Analysis for Sterile 96-Well Plates
Download Certificate of Analysis for Non-Sterile, 96-Well Plates
Download Certificate of Analysis for Sealing Films
Download Certificate of Analysis for IMDM
Download the HALO® Technical Manual
Download the ATP Optimization Kit Protocol for First-Time Users
Download Luminometer Setup and RLU to ATP Conversion
Download Certificate of Analysis (CoAs) for ATP Enumeration Reagent (ATP-ER)
Download Certificate of Analysis for ATP Stanadrds
Download Certificate of Analysis for ATP Controls
Download Certificate of Analysis of ATP Reconstitution Reagent
Download Certificate of Analysis for Sterile 96-Well Plates
Download Certificate of Analysis for Non-Sterile, 96-Well Plates
Download Certificate of Analysis for Sealing Films
Download Certificate of Analysis for IMDM