CAMEO™-96

Complete, Standardized, Methylcellulose Colony-Forming Cell (CFC) or Unit (CFU)
Proliferation and Differentiation Assay for Hematopoietic Stem and Progenitor Cells

 

Buy CAMEO™-96 Assay Kits
 

Complete, standardized, methylcellulose colony-forming cell (CFC) / unit (CFU) proliferation and differentiation assay for hematopoietic stem and progenitor cells from The Colony Experts.

 

  • All applications involving lympho-hematopoietic cells with a manual or image analysis readout.
  • Detect clonal ability of cells.
  • Evaluate the differentiation status or potential of different lympho-hematopoietic cell populations.
  • Correlate in vivo with in vitro differentiation.
  • Differentiation effects of growth factors or cytokines.
  • Evaluate ex vivo differential expansion.
  • Evaluate the effects of drugs, xenobiotics or other agents on the differentiation/maturation process.  
  • CAMEO™-96 is the only complete assay in which both proliferation and differentiation can be determined simultaneously, using different readouts.
  • Allows standardization of the CFU/CFC assay using standards and controls.
  • Allows conversion of colony counts into standardized and quantitative ATP concentrations.
  • Complete flexibility; evaluate growth factors or cytokines.
  • Available for 19 different cell populations and 8 species.
  • Incorporates powerful ATP bioluminescence readout technology to measure cell proliferation.

Complete, Standardized, Methylcellulose Colony-Forming Cell (CFC) or Unit (CFU) Proliferation and Differentiation Assay for Hematopoietic Stem and Progenitor Cells
 
All ATP bioluminescence assays kits from Preferred Cell Systems™ include an ATP standard(s) and controls.  Performing the ATP standard curve and controls is highly recommended. The controls are used to calibrate the instrument and the ATP standard is used to standardize the assay. The values obtained provide the measurement assurance parameters that allow you, the user, to ensure that the results obtained can be trusted.

An example of an ATP standard curve and control results are shown in the diagram below and actual values for the kit being used are given in the Technical Manual. If the values obtained are within the ranges given in the Technical Manual, the user can process the samples knowing that the results will be trustworthy.

Calibration and standardization of an ATP bioluminescence assay from Preferred Cell Systems™ - Measurement Assurance
 
  • Human
  • Non-human primate (Macaca fascicularis (Cynomologus) and Macaca mulatta (Rhesus))
  • Horse
  • Pig
  • Sheep
  • Dog
  • Rat
  • Mouse

The following cell populations are available as CAMEO™-96 Complete Assay Kits.  Those populations that have multiple growth factors will produce different colony types in the same culture. Please note that the number of colonies counted and their proliferation in cultures containing multiple growth factors will be different to those with single growth factors, which only stimulate cells in a specific cell compartment.
 

Cell Type Cell Population Detected (1) MethoCult™ Equivalent Growth Factor Cocktail Suggested Number of Samples(2)
Any None NE None 24, 16 or 12
Stem cell HPP-SP 1 NE 3,6,SCF,Flt3-L 24, 16 or 12
Stem cell HPP-SP 2 NE EPO,GM,2,3,6,7,SCF,TPO, Flt3-L 24, 16 or 12
Stem cell CFC-GEMM 1 NE EPO,GM,3,6,SCF,TPO, Flt3-L 24, 16 or 12
Stem cell CFC-GEMM 2 NE EPO,GM,3,6,SCF,TPO 24, 16 or 12
Stem cell CFC-GEM 1 NE EPO,GM3,6,SCF 24, 16 or 12
Stem cell CFC-GEM 2 H4034 EPO,GM,G,3,SCF 24, 16 or 12
Progenitor BFU-E 1 NE EPO,3,SCF 24, 16 or 12
Progenitor GM-CFC 1 H4534 GM,3,SCF 24, 16 or 12
Progenitor Mk-CFC 1 NE TPO,3,SCF 24, 16 or 12
Progenitor T-CFC NE 2 alone 24, 16 or 12
Progenitor B-CFC NE 7 alone 24, 16 or 12


NE = No equivalent product available.

(1) = Accepted nomenclature of cell populations detected by the colony-forming cell assay.

(2) = The number of samples is based on the number of replicate wells/sample. If 4 replicate wells/sample are used, 24 samples/plate can be detected. Using 6 replicate wells/sample, 16 samples can be detected and for 8 replicates wells/samples prepared in columns, 12 samples can be measured.

When ordering CAMEO™-96, please use the Catalog Number and Species. You can quickly find these products by copying the catalog number and entering it in the advanced search.
Advanced Search

 

Cell Population Human Primate Dog Rat Mouse
None KCO-0-1H KCO-0-1Pr KCO-0-1C KCO-0-1R KCO-0-1M
HPP-SP1 KCO-HPP1-1H KCO-HPP1-1Pr KCO-HPP1-1C KCO-HPP1-1R KCO-HPP1-1M
HPP-SP2 KCO-HPP2-1H KCO-HPP2-1Pr KCO-HPP2-1C KCO-HPP2-1R KCO-HPP2-1M
CFC-GEMM1 KCO-GEMM1-1H KCO-GEMM1-1Pr KCO-GEMM1-1C KCO-GEMM1-1R KCO-GEMM1-1M
CFC-GEMM2 KCO-GEMM2-1H KCO-GEMM2-1Pr KCO-GEMM2-1C KCO-GEMM2-1R KCO-GEMM2-1M
CFC-GEM1 KCO-GEM1-1H KCO-GEM1-1Pr KCO-GEM1-1C KCO-GEM1-1R KCO-GEM1-1M
CFC-GEM2 KCO-GEM2-1H KCO-GEM2-1Pr KCO-GEM2-1C KCO-GEM2-1R KCO-GEM2-1M
BFU-E1 KCO-BFU1-1H KCO-BFU1-1Pr KCO-BFU1-1C KCO-BFU1-1R KCO-BFU1-1M
GM-CFC1 KCO-GM1-1H KCO-GM1-1Pr KCO-GM1-1C KCO-GM1-1R KCO-GM1-1M
MK-CFC1 KCO-Mk1-1H KCO-Mk1-1Pr KCO-Mk1-1C KCO-Mk1-1R KCO-Mk1-1M
T-CFC KCO-T-1H KCO-T-1Pr NA KCO-T-1R KCO-T-1M
B-CFC KCO-B-1H KCO-B-1Pr NA NA KCO-B-1M

 

Cell Population Horse Pig Sheep
None KCO-0-1E KCO-0-1P KCO-0-1O
HPP-SP1 KCO-HPP1-1E KCO-HPP1-1P KCO-HPP1-1O
HPP-SP2 KCO-HPP2-1E KCO-HPP2-1P KCO-HPP2-1O
CFC-GEMM1 KCO-GEMM1-1E KCO-GEMM1-1P KCO-GEMM1-1O
CFC-GEMM2 KCO-GEMM2-1E KCO-GEMM2-1P KCO-GEMM2-1O
CFC-GEM1 KCO-GEM1-1E KCO-GEM1-1P KCO-GEM1-1O
CFC-GEM2 KCO-GEM2-1E KCO-GEM2-1P KCO-GEM2-1O
BFU-E1 KCO-BFU1-1E KCO-BFU1-1P KCO-BFU1-1O
GM-CFC1 KCO-GM1-1E KCO-GM1-1P KCO-GM1-1O
MK-CFC1 KCO-Mk1-1E KCO-Mk1-1P KCO-Mk1-1O
For Research Use Only. Not for clinical diagnostic use.

Inverted microscope with a magnification of 40x - 100x.
Luminescence plate reader or multimode plate reader with "glow" luminescence capability.
  • CAMEO™-96 Reagent
  • ATP standard
  • ATP high and low controls
  • ATP Enumeration Reagent
  • Sterile, 96-well plates
  • Non-sterile-96-well plates
  • Sterile, adhesive foil covers

Complete, standardized, methylcellulose colony-forming cell (CFC) / unit (CFU) proliferation and differentiation assay for hematopoietic stem and progenitor cells.

Download the CAMEO™-96 Technical Manual
Download Luminometer Setup and RLU to ATP Conversion
Download Certificate of Analysis (CoAs) for ATP Bioluminescence Components. Note: Some CoAs will be included with assay kit
Download methylcellulose SDS sheet
Download ATP standards and controls SDS sheet
Download ATP Enumeration Reagents (ATP-ER) SDS sheet