ImmunoGlo™-Tox HT Assays

Complete and Standardized ATP Bioluminescence Immunotoxicity Assays Including Specialized Assays for T- and B-Lymphocytes
 

Buy ImmunoGlo™-Tox HT Assay Kits
 

A complete and standardized bioluminescence lymphocyte / immune toxicity assay platform.

 

  • ImmunoGlo™-Tox HT is a general use functional immune cell proliferation assay where the investigator employs their own culture reagents and protocol for any type of immune cell.
  • ImmunoGlo™ Tox HT TCP Assay Kits include specific cocktails to stimulate functional T-lymphocytes.
  • ImmunoGlo™ Tox HT BCP is specific for stimulating functional human B-lymphocytes.
  • In vitro high-throughput immunotoxicity screening.
  • In vitro high throughput lymphocyte proliferation screening.
  • Specialized toxicity assays for T- and B-lymphocytes.
  • Cellular immune response screening.
  • Development of drugs and immunotherapies using gene targeting, e.g. CRISPR-Cas9.
  • Non-radioactive assay incorporating proven high-throughput ATP bioluminescence technology.
  • Instrument-based, quantitative and fully standardized allowing comparison of results over time.
  • Can be validated for GLP studies.
  • More sensitive than WST-1, CSFE and other absorbance or fluorescence assays.
  • Available in 96- or 384-well plate formats.
  • Multiplexing capability to incorporate flow cytometry, cytokine release and other assay readouts for additional information from the same sample.
  • Simple, time efficient and cost effective. 

Principle of ATP Bioluminescence Cell Proliferation/Cytotoxicity Assays

All ATP bioluminescence assays kits from Preferred Cell Systems™ include an ATP standard(s) and controls.  Performing the ATP standard curve and controls is highly recommended. The controls are used to calibrate the instrument and the ATP standard is used to standardize the assay. The values obtained provide the measurement assurance parameters that allow you, the user, to ensure that the results obtained can be trusted.

An example of an ATP standard curve and control results are shown in the diagram below and actual values for the kit being used are given in the Technical Manual. If the values obtained are within the ranges given in the Technical Manual, the user can process the samples knowing that the results will be trustworthy.

Calibration and standardization of an ATP bioluminescence assay from Preferred Cell Systems™ - Measurement Assurance
 
ImmunoGlo™-Tox HT can be used for multiple species, including:
  • Human
  • Primate
  • Rat
  • Mouse
  • Peripheral blood mononuclear cells (PBMC)
  • Primary lymphocyte sub-populations
  • Primary organs and tissues containing immune cells
  • Lymphocyte cell lines
Assay Type Catalog Number ImmunoGro™ Medium containing: Number of Plates/
ImmunoGlo™-Tox HT KM1-96-2 or 4 No cytokines or co-stimulators 2 or 4
ImmunoGlo™-Tox HT TCP KM1-T96CS1-2H IL-2 alone (may require co-stimulators) 2
ImmunoGlo™-Tox HT TCP KM1-T96CS1-2Pr IL-2 alone (may require co-stimulators) 2
ImmunoGlo™-Tox HT TCP KM1-T96CS1-2R IL-2 alone (may require co-stimulators) 2
ImmunoGlo™-Tox HT TCP KM1-T96CS1-2M IL-2 alone (may require co-stimulators) 2
ImmunoGlo™-Tox HT TCP KM1-T96CS2-2H CD3 CD28 2
ImmunoGlo™-Tox HT TCP KM1-T96CS3-2H IL-2 CD3 CD28 2
ImmunoGlo™-Tox HT BCP KM1-B96C2-2H Cocktail includes CD40L and IL-4 2
ImmunoGlo™-Tox HT BCP KM1-B96C3-2H Cocktail includes IL-4 2

Human cell assays (H).  Primate cell assays (Pr). Rat cell assays (R). Mouse cell assays (M).

For Research Use Only.

Luminescence or multimode plate reader with "glow" luminescence measuring capability.

ImmunoGlo™-Tox HT
  • Base medium for ATP standard dilution
  • ATP standard
  • ATP high and low controls
  • ATP Enumeration Reagent
  • Sterile, 96- or 384-well culture plates
  • Non-sterile, 96- or 384-well plates
ImmunoGlo™-Tox HT TCP
  • ImmunoGlo™ TCPMaster Mix for requested T-lymphocyte stimulation
  • Base medium for ATP standard dilution
  • ATP standard
  • ATP high and low controls
  • ATP Enumeration Reagent
  • Sterile, 96- or 384-well culture plates
  • Non-sterile, 96- or 384-well plates
ImmunoGlo™-Tox HT BCP
  • ImmunoGlo™ BCP Master Mix for requested B-lymphocyte stimulation
  • Base medium for ATP standard dilution
  • ATP standard
  • ATP high and low controls
  • ATP Enumeration Reagent
  • Sterile, 96- or 384-well culture plates
  • Non-sterile, 96- or 384-well plates
ImmunoGlo™-Tox HT Assay Manual

A complete and standardized bioluminescence lymphocyte / immune toxicity assay platform.