Standardized Bioluminescence Toxicity Testing Assay for Multiple Cell Types


Buy STEMGlo™-Tox HT Assay Kits

A standardized ATP bioluminescence toxicity testing assay platform for primary explanted cells, ES, iPS, cell lines and tumor cells.


  • High throughput in vitro toxicity screening and testing of multiple cell types with multiplexing capability.
  • Can be incorporated at any stage of drug development, including ADME/Tox.
  • Drug/agent toxicity ranking according to cell source and species.
  • Development of drugs and other therapies using stem cell gene targeting and expression research, e.g. CRISPR-Cas9.
  • A flexible, in vitro assay platform to perform high throughput toxicity testing.
  • Can be applied to numerous cell types derived from different organs, tissues and species.
  • High-throughput capability using 96- or 384-well plate formats allows ADME-Tox drug or compound screening, thereby significantly reducing unexpected results during pre-clinical testing.
  • A 3Rs Assay Platform-Helps Reduction, Refinement, Replacement for animal testing.
  • Available for adherent or non-adherent cell types.
  • 2D and 3D cell culture models.
  • Incorporates the most sensitive ATP bioluminescence readout available to measure proliferation, cytotoxicity, cell number and even apoptosis.
  • Kits include standards and controls to calibrate and standardize the assay.
  • Validated signal detection readout according to FDA Bioanalytical Method Guidelines.
  • Allows results to be compared over time.
  • Results usually in 4 to 5 days depending on species and cell type.
  • Designed for multiplexing with other assay using the same sample.
  • Simple, time efficient and cost effective.

Principle of ATP Bioluminescence Cell Proliferation/Cytotoxicity Assays

All ATP bioluminescence assays kits from Preferred Cell Systems™ include an ATP standard(s) and controls.  Performing the ATP standard curve and controls is highly recommended. The controls are used to calibrate the instrument and the ATP standard is used to standardize the assay. The values obtained provide the measurement assurance parameters that allow you, the user, to ensure that the results obtained can be trusted.

An example of an ATP standard curve and control results are shown in the diagram below and actual values for the kit being used are given in the Technical Manual. If the values obtained are within the ranges given in the Technical Manual, the user can process the samples knowing that the results will be trustworthy.

Calibration and standardization of an ATP bioluminescence assay from Preferred Cell Systems™ - Measurement Assurance
  • Multiple


Cell Types

  • Bladder
  • Brain
  • Breast
  • Eye
  • Gut
  • Heart
  • Kidney
  • Lung
  • Liver
  • Ovary
  • Prostate
  • Skin
  • Embryonic tissue
  • Fetal tissue


Other Cell Types Include:

  • Proliferating cell lines
  • Tumor cells
  • Embryonic stem (ES) cells
  • Induce pluripotent stem (iPS) cells

When ordering STEMGlo™-Tox HT, please use the Catalog Number and Species. You can quickly find these products by copying the catalog number and pasting it in the advanced search.
Advanced Search

STEMGlo™-Tox HT Assay Kits

Cell Type Included Catalog Number Assay Plate Type Number of Plates
Ex vivo, ES, iPS, transformed cell lines and tumor cell lines KSG-T96-A-2 Adherent 2
Ex vivo, ES, iPS, transformed cell lines and tumor cell lines KSG-T96-A-4 Adherent 4
Ex vivo, ES, iPS, transformed cell lines and tumor cell lines KSG-T96-NA-2 Non-adherent 2
Ex vivo, ES, iPS, transformed cell lines and tumor cell lines KSG-T96-NA-4 Non-adherent 4

Larger quantities available upon request.
STEMGlo™-Tox HT is also available in 384-well plate format. Please contact Preferred Cell Systems™ for more information.


For Research Use Only. Not for clinical diagnostic use.
Luminescence or multimode plate reader with "glow" luminescence measuring capability.

  • ATP standard
  • ATP controls
  • Medium
  • ATP Enumeration Reagent
  • Sterile, 96- or 384-well plates, depending on assay use
  • Non-sterile, 96- or 384-well plates, depending on assay use
  • Sterile, adhesive foil covers to maintain sterility of unused wells

STEMGlo™-Tox HT Assay Manual

Standardized Bioluminescence Toxicity Testing Assay for Multiple Cell Types