STEMGlo™-Tox HT

Fully Standardized ATP Bioluminescence In Vitro Toxicity Assay Platform for ES, iPS or other stem cell lines.

 

Buy STEMGlo™-Tox HT Assay Kits
 

A standardized ATP bioluminescence toxicity testing assay platform for primary explanted cells, ES, iPS, cell lines and tumor cells.

 

  • High throughput in vitro toxicity screening and testing using embryonic stem (ES), induced pluripotent stem (iPS) cells or other stem cell lines.
  • Can be incorporated at any stage of drug development, including ADME/Tox.
  • Drug/agent toxicity ranking according to cell source and species.
  • Development of drugs and other therapies using stem cell gene targeting and expression research, e.g. CRISPR-Cas9.
  • A flexible, in vitro assay platform to perform high throughput toxicity testing.
  • Can be applied to numerous cell types derived from different organs, tissues and species.
  • High-throughput capability using 96- or 384-well plate formats allows ADME-Tox drug or compound screening, thereby significantly reducing unexpected results during pre-clinical testing.
  • A 3Rs Assay Platform-Helps Reduction, Refinement, Replacement for animal testing.
  • Available for adherent or non-adherent cell types.
  • 2D and 3D cell culture models.
  • Incorporates the most sensitive ATP bioluminescence readout available to measure proliferation, cytotoxicity, cell number and even apoptosis.
  • Kits include standards and controls to calibrate and standardize the assay.
  • Validated signal detection readout according to FDA Bioanalytical Method Guidelines.
  • Allows results to be compared over time.
  • Results usually in 4 to 5 days depending on species and cell type.
  • Designed for multiplexing with other assay using the same sample.
  • Simple, time efficient and cost effective.

Principle of ATP Bioluminescence Cell Proliferation/Cytotoxicity Assays

All ATP bioluminescence assays kits from Preferred Cell Systems™ include an ATP standard(s) and controls.  Performing the ATP standard curve and controls is highly recommended. The controls are used to calibrate the instrument and the ATP standard is used to standardize the assay. The values obtained provide the measurement assurance parameters that allow you, the user, to ensure that the results obtained can be trusted.

An example of an ATP standard curve and control results are shown in the diagram below and actual values for the kit being used are given in the Technical Manual. If the values obtained are within the ranges given in the Technical Manual, the user can process the samples knowing that the results will be trustworthy.

Calibration and standardization of an ATP bioluminescence assay from Preferred Cell Systems™ - Measurement Assurance
 
STEMGlo™-Tox HT can be used to investigate potential in vitro toxicity using ES, iPS or other stem cell lines for different species.

For Research Use Only. Not for clinical diagnostic use.
Luminescence or multimode plate reader with "glow" luminescence measuring capability.

  • ATP standard
  • ATP controls
  • Medium
  • ATP Enumeration Reagent
  • Sterile, white solid, 96-well plates. Available for adherent or non-adherent cells.
  • Non-sterile, 96-well plates for assay calibration and standardization.
  • Sterile, adhesive foil covers to maintain sterility of unused wells

STEMGlo™-Tox HT Assay Manual

Standardized Bioluminescence Toxicity Testing Assay for Multiple Cell Types

When ordering STEMGlo™-Tox HT, simply click the Catalog Number in the table below and you will be taken directly to the desired product.
 

STEMGlo™-Tox HT Assay Kits

Cell Type Included Catalog Number Assay Plate Type Number of Plates
ES, iPS and other stem cell lines KSG-T96A-2 96-well plate. 0.1mL/well assay 2
ES, iPS and other stem cell lines KSG-T96A-4 96-well plate. 0.1mL/well assay 4
ES, iPS and other stem cell lines KSG-T96NA-2 96-well plate. 0.1mL/well assay 2
ES, iPS and other stem cell lines KSG-T96NA-4 96-well plate. 0.1mL/well assay 4

Larger quantities available upon request.
STEMGlo™-Tox HT is also available in 384-well plate format. Please contact Preferred Cell Systems™ for more information.