HALO®-Tox HT

Predictive Bone Marrow Toxicity / Stem Cell Hematotoxicity ATP Bioluminescence Assays
 

Buy HALO®-Tox HT Assay Kits

Buy HALO®-Tox HT 4 Population

Buy HALO®-Tox HT 5 Population


Buy HALO®-Tox HT 7 Population



The most advanced and complete bone marrow toxicity / stem cell hematotoxicity / myelotoxicity assays using an ATP bioluminescence endpoint.

  • Predictive in vitro to in vivo surrogate assay for human and animal hematotoxicity/hemotoxicity testing.
  • Predictive in vitro stem and progenitor hematotoxicity screening and testing platform for all stages of drug development, including patient monitoring during clinical trials.
  • Predictive in vitro stem and progenitor hematotoxicity screening and testing for xenobiotic agents.
  • Incorporation into routine high throughput ADME/Tox screening.
  • Drug / compound ranking according to species or cell population sensitivity.
  • Gene targeting and expression for new drug development and therapies, e.g. CRISPR-Cas9.
  • Incorporates proven, standardized and validated ATP bioluminescence technology, the most sensitive signal detection system available.
  • All assay kits include everything needed to culture the required cell population and all reagents to calibrate and standardize the assay as well as 96-well plates. Assay kits are also available with 384-well plates upon request.
  • Measurement assurance parameters included to ensure the assay is working correctly.
  • Reliably compare results over time and between drug lots.
  • Highly predictive with greater than 80% concordance between in vitro and in vivo results (Toxicol Lett. 2009; 188:98-103).
  • 3Rs Alternative Assay Platform for Reduction, Refinement and Replacement of animal testing.
  • Rapid turnaround: results in 5 days for human and non-human primate cells, and 4 days for other animal cells.
  • Validated according to FDA Bioanalytical Method Validation Guidelines and against the Registry of Cytotoxicity Prediction Model as a cytotoxicity assay.
  • Estimate in vivo starting doses and maximum tolerated doses (MTD) from in vitro IC values.
  • Incorporates Suspension Expansion Culture™ (SEC™) technology for the most advanced and reliable hematotoxicity assay available.
  • Designed for multiplexing with other assays.
  • Simple and time efficient.

Principle of ATP Bioluminescence Cell Proliferation/Cytotoxicity Assays

All ATP bioluminescence assays kits from Preferred Cell Systems™ include an ATP standard(s) and controls.  Performing the ATP standard curve and controls is highly recommended. The controls are used to calibrate the instrument and the ATP standard is used to standardize the assay. The values obtained provide the measurement assurance parameters that allow you, the user, to ensure that the results obtained can be trusted.

An example of an ATP standard curve and control results are shown in the diagram below and actual values for the kit being used are given in the Technical Manual. If the values obtained are within the ranges given in the Technical Manual, the user can process the samples knowing that the results will be trustworthy.

Calibration and standardization of an ATP bioluminescence assay from Preferred Cell Systems™ - Measurement Assurance
 
  • Human
  • Non-human primate (Cynomologus and Rhesus)
  • Dog
  • Rat
  • Mouse
  • Horse, pig and sheep also available as custom orders
  • Bone marrow; human and animal tissues
  • Peripheral blood; human and animal tissues
  • Umbilical cord blood; human and some animals
  • Spleen; rodents
  • Fetal and adult liver; rodents
  • Yolk sac; rodents

Stem Cells

SC-HPP 2: EPO, GM-CSF, IL-2, IL-3, Il-6, IL-7, SCF, TPO, Flt3-L

SC-GEMM 1: EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L

Progenitor Cells

• P- BFU 1: EPO, IL-3, SCF

P-GM 1: GM-CSF, IL-3, SCF

P-Mk 1: TPO, IL-3, SCF

Lymphopoietic Cells

•  P-Tcell 1: IL-2 (requires co-stimulators, primate, dog, rat, mouse only)

•  P-Tcell 3: IL-2 co-stimulators CD3 CD28 (human only)

•  P-Bcell 1: IL-7 (requires co-stimulators, primate, dog, rat only)

•  P-Bcell 3: Cocktail includes IgD-Dex and cytokines (human and mouse)

 

No growth factors: Add growth factors/cytokines of choice.

 

 

Cell Populations for "Global" Hematotoxicity Testing

 

4 Population Hemotoxicity Assay Kits (4 Pop)

  • SC-GEMM 1
  • P-BFU 1
  • P-GM 1
  • P-Mk 1
  • Background control

 

5 Population Hematotoxicity Assay Kits (5 Pop)

  • SC-HPP 2
  • SC-GEMM 1
  • P-BFU 1
  • P-GM 1
  • P-Mk 1
  • Background control
 

7 Population Lympho-Hematotoxicity Assay Kits (7 Pop)

  • SC-HPP 2
  • SC-GEMM 1
  • P-BFU 1
  • P-GM 1
  • P-Mk 1
  • P-Tcell 1 or 3 (depending on species)
  • P-Bcell 1 or 3 (depending on species)
  • Background control
For Research Use Only.

Luminescence or multimode plate reader with "glow" luminescence measuring capability.
  • HALO®-Tox HT Master Mix
  • ATP standard
  • ATP high and low controls
  • ATP Enumeration Reagent
  • Sterile, 96-well plates
  • Non-sterile, 96-well plates

 

Download the manual for HALO®-Tox HT
Download the Luminometer Setup and RLU to ATP Conversion Protocol
Download Certificate of Analysis (CoAs) for ATP bioluminescence components. Note: Some of CoAs will be included with the assay kits.
Download SDS for SEC Media for Kits
Download SDS for ATP Standard and Controls
Download SDS for ATP Enumeration Reagent

When ordering HALO®-Tox HT, simply click on Catalog Number Search in the tables below and you will be taken directly to the desired product.

LOW SERUM 2-PLATE ASSAY KITS

Cell Population Human Primate Dog Rat Mouse
None K2-0-2HH K2-0-2PrH K2-0-2CH K2-0-2RH K2-0-2MH
P-Bcell 1 K2-B1-2HH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2-B1-2HH-NAX" K2-B1-2PrH K2-B1-2CH K2-B1-2RH K2-B1-2HH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2-B1-2HH-NAX"
P-Bcell 3 K2-B3-2HH K2-B3-2PrH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2-B3-2PrH-NAX" K2-B3-2CH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2-B3-2CH-NAX" K2-B3-2RH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2-B3-2RH-NAX" K2-B3-2MH
P-BFU 1 K2-BFU1-2HH K2-BFU1-2PrH K2-BFU1-2CH K2-BFU1-2RH K2-BFU1-2MH
P-GM 1 K2-GM1-2HH K2-GM1-2PrH K2-GM1-2CH K2-GM1-2RH K2-GM1-2MH
P-Mk 1 K2-Mk1-2HH K2-Mk1-2PrH K2-Mk1-2CH K2-Mk1-2RH K2-Mk1-2MH
P-Tcell 1 K2-B1-2HH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2-B1-2HH-NAX" K2-T1-2PrH K2-T1-2CH K2-T1-2RH K2-T1-2MH
P-Tcell 3 K2-T3-2HH K2-T3-2PrH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2-T3-2PrH-NAX" K2-B1-2CH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2-B1-2CH-NAX" K2-B1-2RH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2-B1-2RH-NAX" K2-B1-2MH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2-B1-2MH-NAX"
SC-GEMM 1 K2-GEMM1-2HH K2-GEMM1-2PrH K2-GEMM1-2CH K2-GEMM1-2RH K2-GEMM1-2MH
SC-HPP 2 K2-HPP2-2HH K2-HPP2-2PrH K2-HPP2-2CH K2-HPP2-2RH K2-HPP2-2MH

 

LOW SERUM, 4-PLATE ASSAY KITS

Cell Population Human Primate Dog Rat Mouse
None K2-0-4HH K2-0-4PrH K2-0-4CH K2-0-4RH K2-0-4MH
P-Bcell 1 K2SF-B1-2HH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2SF-B1-2HH-NAX" K2-B1-4PrH K2-B1-4CH K2-B1-4RH K2SF-B1-2MH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2SF-B1-2MH-NAX"
P-Bcell 3 K2-B3-4HH K2SF-T3-2MH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2SF-T3-2MH-NAX" K2SF-T3-2MH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2SF-T3-2MH-NAX" K2SF-T3-2MH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2SF-T3-2MH-NAX" K2-B3-4MH
P-BFU 1 K2-BFU1-4HH K2-BFU1-4PrH K2-BFU1-4CH K2-BFU1-4RH K2-BFU1-4MH
P-GM 1 K2-GM1-4HH K2-GM1-4PrH K2-GM1-4CH K2-GM1-4RH K2-GM1-4MH
P-Mk 1 K2-Mk1-4HH K2-Mk1-4PrH K2-Mk1-4CH K2-Mk1-4RH K2-Mk1-4MH
P-Tcell 1 K2-T1-4HH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2-T1-4HH-NAX" K2-T1-4PrH K2-T1-4CH K2-T1-4RH K2-T1-4MH
P-Tcell 3 K2-T3-4HH K2-T3-4PrH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2-T3-4PrH-NAX" K2-T3-4CH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2-T3-4CH-NAX" K2-T3-4RH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2-T3-4RH-NAX" K2-T3-4MH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2-T3-4MH-NAX"
SC-GEMM 1 K2-GEMM1-4HH K2-GEMM1-4PrH K2-GEMM1-4CH K2-GEMM1-4RH K2-GEMM1-4MH
SC-HPP 2 K2-HPP2-4HH K2-HPP2-4PrH K2-HPP2-4CH K2-HPP2-4RH K2-HPP2-4MH

 

LOW SERUM "GLOBAL" POPULATION ASSAY KITS

Cell Population Human Primate Dog Rat Mouse
"Global" 4-Pop Tox K2-4P-5HH K2-4P-5PrH K2-4P-5CH K2-4P-5RH K2-4P-5MH
"Global" 5-Pop Tox K2-5P-6HH K2-5P-6PrH K2-5P-6CH K2-5P-6RH K2-5P-6MH
"Global" 7-Pop Tox K2-7P-8HH K2-7P-8PrH K2-7P-8CH K2-7P-8RH K2-7P-8MH

 

 

SERUM-FREE, 2-PLATE ASSAY KITS

Cell Population Human Primate Mouse
None K2SF-0-2HH K2SF-0-2PrH K2SF-0-2MH
P-Bcell 1 K2SF-B1-2HH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2SF-B1-2HH-NAX" K2SF-B1-2PrH K2SF-B1-2MH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2SF-B1-2MH-NAX"
P-Bcell 3 K2SF-B3-2HH K2SF-B3-2PrH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2SF-B3-2PrH-NAX" K2SF-B3-2MH
P-BFU 1 K2SF-BFU1-2HH K2SF-BFU1-2PrH K2SF-BFU1-2MH
P-GM 1 K2SF-GM1-2HH K2SF-GM1-2PrH K2SF-GM1-2MH
P-Mk 1 K2SF-Mk1-2HH K2SF-Mk1-2PrH K2SF-Mk1-2MH
P-Tcell 1 K2SF-B3-2PrH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2SF-B3-2PrH-NAX" K2SF-T1-2PrH K2SF-T1-2MH
P-Tcell 3 K2SF-T3-2HH K2SF-B3-2PrH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2SF-B3-2PrH-NAX" K2SF-B3-2PrH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2SF-B3-2PrH-NAX"
SC-GEMM 1 K2SF-GEMM1-2HH K2SF-GEMM1-2PrH K2SF-GEMM1-2MH
SC-HPP 2 K2SF-HPP2-2HH K2SF-HPP2-2PrH K2SF-HPP2-2MH

 

SERUM-FREE, 4-PLATE ASSAY KITS

Cell Population Human Primate Mouse
None K2SF-0-4HH K2SF-0-4PrH K2SF-0-4MH
P-Bcell 1 K2SF-B1-4HH K2SF-B1-4PrH K2SF-B1-4MH
P-BFU 1 K2SF-BFU1-4HH K2SF-BFU1-4PrH K2SF-BFU1-4MH
P-GM 1 K2SF-GM1-4HH K2SF-GM1-4PrH K2SF-GM1-4MH
P-Mk 1 K2SF-Mk1-4HH K2SF-Mk1-4PrH K2SF-Mk1-4MH
P-Tcell 1 K2SF-T1-2HH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2SF-T1-2HH-NAX" K2SF-T1-4PrH K2SF-T1-4MH
P-Tcell 3 K2SF-T3-4HH K2SF-T3-2PrH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2SF-T3-2PrH-NAX" K2SF-T3-2MH-NAX"&Search.x=33&Search.y=4&Search=Search">"K2SF-T3-2MH-NAX"
SC-GEMM 1 K2SF-GEMM1-4HH K2SF-GEMM1-4PrH K2SF-GEMM1-4MH
SC-HPP 2 K2SF-HPP2-4HH K2SF-HPP2-4PrH K2SF-HPP2-4MH

 

SERUM-FREE,"GLOBAL" POPULATION ASSAY KITS

Cell Population Human Primate Mouse
"Global" 4-Pop Tox K2SF-4P-5HH K2SF-4P-5PrH K2SF-4P-5MH
"Global" 5-Pop Tox K2SF-5P-6HH K2SF-5P-6PrH K2SF-5P-6MH
"Global" 7-Pop Tox K2SF-7P-8HH K2SF-7P-8PrH K2SF-7P-8MH