HALO®-Tox HT

Bone Marrow Toxicity / Stem Cell Hematotoxicity / Myelotoxicity Bioluminescence Cytotoxicity, Functional and Viability Assays 
 

Buy HALO®-Tox HT Assay Kits

Buy HALO®-Tox HT 4 Population

Buy HALO®-Tox HT 5 Population


Buy HALO®-Tox HT 7 Population



The most advanced and complete bone marrow toxicity / stem cell hematotoxicity / myelotoxicity assays using an ATP bioluminescence endpoint.

  • Predictive in vitro to in vivo surrogate assay for human and animal hematotoxicity/hemotoxicity testing.
  • Predictive in vitro stem and progenitor hematotoxicity screening and testing platform for all stages of drug development, including patient monitoring during clinical trials.
  • Predictive in vitro stem and progenitor hematotoxicity screening and testing for xenobiotic agents.
  • Incorporation into routine high throughput ADME/Tox screening.
  • Drug / compound ranking according to species or cell population sensitivity.
  • Gene targeting and expression for new drug development and therapies, e.g. CRISPR-Cas9.
  • 3Rs Alternative Assay Platform for Reduction, Refinement and Replacement of animal testing.
  • The only in vitro high throughput stem and progenitor hemotoxicity assay platform available with both 96-well and 384-well plate formats.
  • Highly predictive with greater than 80% concordance between in vitro and in vivo results (Toxicol Lett. 2009; 188:98-103).
  • Rapid turnaround: results in 5 days for human and non-human primate cells, and 4 days for other animal cells.
  • Validated against the Registry of Cytotoxicity Prediction Model as a cytotoxicity assay.
  • Estimate in vivo starting doses and maximum tolerated doses (MTD) from in vitro IC values.
  • Incorporates Suspension Expansion Culture and proven ATP bioluminescence technology for the most advanced and reliable hematotoxicity assay available.
  • Designed for multiplexing with other assays.

Principle of ATP Bioluminescence Cell Proliferation/Cytotoxicity Assays

All ATP bioluminescence assays kits from Preferred Cell Systems™ include an ATP standard(s) and controls.  Performing the ATP standard curve and controls is highly recommended. The controls are used to calibrate the instrument and the ATP standard is used to standardize the assay. The values obtained provide the measurement assurance parameters that allow you, the user, to ensure that the results obtained can be trusted.

An example of an ATP standard curve and control results are shown in the diagram below and actual values for the kit being used are given in the Technical Manual. If the values obtained are within the ranges given in the Technical Manual, the user can process the samples knowing that the results will be trustworthy.

Calibration and standardization of an ATP bioluminescence assay from Preferred Cell Systems™ - Measurement Assurance
 
  • Human
  • Non-human primate (Cynomologus and Rhesus)
  • Dog
  • Rat
  • Mouse
  • Horse, pig and sheep also available as custom orders
  • Bone marrow; human and animal tissues
  • Peripheral blood; human and animal tissues
  • Umbilical cord blood; human and some animals
  • Spleen; rodents
  • Fetal and adult liver; rodents
  • Yolk sac; rodents

Stem Cells

SC-HPP 2: EPO, GM-CSF, IL-2, IL-3, Il-6, IL-7, SCF, TPO, Flt3-L

SC-GEMM 1: EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L

Progenitor Cells

• P- BFU 1: EPO, IL-3, SCF

P-GM 1: GM-CSF, IL-3, SCF

P-Mk 1: TPO, IL-3, SCF

Lymphopoietic Cells

•  P-Tcell 1: IL-2 (requires co-stimulators, primate, dog, rat, mouse only)

•  P-Tcell 3: IL-2 co-stimulators CD3 CD28 (human only)

•  P-Bcell 1: IL-7 (requires co-stimulators, primate, dog, rat, mouse only)

•  P-Bcell 3: Includes CD40 ligand and IL-4 (human only)

 

No growth factors: Add growth factors/cytokines of choice.

 

 

Cell Populations for "Global" Hematotoxicity Testing

 

4 Population Hemotoxicity Assay Kits (4 Pop)

  • SC-GEMM 1
  • P-BFU 1
  • P-GM 1
  • P-Mk 1
  • Background control

 

5 Population Hematotoxicity Assay Kits (5 Pop)

  • SC-HPP 2
  • SC-GEMM 1
  • P-BFU 1
  • P-GM 1
  • P-Mk 1
  • Background control
 

7 Population Lympho-Hematotoxicity Assay Kits (7 Pop)

  • SC-HPP 2
  • SC-GEMM 1
  • P-BFU 1
  • P-GM 1
  • P-Mk 1
  • P-Tcell 1 or 3 (depending on species)
  • P-Bcell 1 or 3 (depending on species)
  • Background control

When ordering HALO® Tox HT, please use the Catalog Number and Species. You can quickly find these products by copying the catalog number and pasting it in the advanced search.
Advanced Search

LOW SERUM 2-PLATE ASSAY KITS

Cell Population Human Primate Dog Rat Mouse
None K2-0-2HH K2-0-2PrH K2-0-2CH K2-0-2RH K2-0-2MH
SC-HPP 2 K2-HPP2-2HH K2-HPP2-2PrH K2-HPP2-2CH K2-HPP2-2RH K2-HPP2-2MH
SC-GEMM 1 K2-GEMM1-2HH K2-GEMM1-2PrH K2-GEMM1-2CH K2-GEMM1-2RH K2-GEMM1-2MH
P-BFU 1 K2-BFU1-2HH K2-BFU1-2PrH K2-BFU1-2CH K2-BFU1-2RH K2-BFU1-2MH
P-GM 1 K2-GM1-2HH K2-GM1-2PrH K2-GM1-2CH K2-GM1-2RH K2-GM1-2MH
P-MK 1 K2-Mk1-2HH K2-Mk1-2PrH K2-Mk1-2CH K2-Mk1-2RH K2-Mk1-2MH
P-Tcell 1 NA K2-T1-2PrH K2-T1-2CH K2-T1-2RH K2-T1-2MH
P-Tcell 3 K2-T3-2HH NA NA NA NA
P-Bcell 1 NA K2-B1-2PrH K2-B1-2CH K2-B1-2RH K2-B1-2MH
P-Bcell 3 K2-B3-2HH NA NA NA NA

 

LOW SERUM, 4-PLATE ASSAY KITS

Cell Population Human Primate Dog Rat Mouse
None K2-0-4HH K2-0-4PrH K2-0-4CH K2-0-4RH K2-0-4MH
SC-HPP 2 K2-HPP2-4HH K2-HPP2-4PrH K2-HPP2-4CH K2-HPP2-4RH K2-HPP2-4MH
SC-GEMM 1 K2-GEMM1-4HH K2-GEMM1-4PrH K2-GEMM1-4CH K2-GEMM1-4RH K2-GEMM1-4MH
P-BFU 1 K2-BFU1-4HH K2-BFU1-4PrH K2-BFU1-4CH K2-BFU1-4RH K2-BFU1-4MH
P-GM 1 K2-GM1-4HH K2-GM1-4PrH K2-GM1-4CH K2-GM1-4RH K2-GM1-4MH
P-MK 1 K2-Mk1-4HH K2-Mk1-4PrH K2-Mk1-4CH K2-Mk1-4RH K2-Mk1-4MH
P-Tcell 1 NA K2-T1-4PrH K2-T1-4CH K2-T1-4RH K2-T1-4MH
P-Tcell 3 K2-T3-4HH NA NA NA NA
P-Bcell 1 NA K2-B1-4PrH K2-B1-4CH K2-B1-4RH K2-B1-4MH
P-Bcell 3 K2-B3-4HH NA NA NA NA

 

LOW SERUM "GLOBAL" POPULATION ASSAY KITS

Cell Population Human Primate Dog Rat Mouse
"Global" 4-Pop K2-4P-5HH K2-4P-5PrH K2-4P-5CH K2-4P-5RH K2-4P-5MH
"Global" 5-Pop K2-5P-6HH K2-5P-6PrH K2-5P-6CH K2-5P-6RH K2-5P-6MH
"Global" 7-Pop K2-7P-8HH K2-7P-8PrH K2-7P-8CH K2-7P-8RH K2-7P-8MH

 

 

SERUM-FREE, 2-PLATE ASSAY KITS

Cell Population Human Primate Mouse
None K2SF-0-2HH K2SF-0-2HPr K2SF-0-2HM
SC-HPP 2 K2SF-HPP2-2HH K2SF-HPP2-2HPr K2SF-HPP2-2HM
SC-GEMM 1 K2SF-GEMM1-2HH K2SF-GEMM1-2HPr K2SF-GEMM1-2HM
P-BFU 1 K2SF-BFU1-2HH K2SF-BFU1-2HPr K2SF-BFU1-2HM
P-GM 1 K2SF-GM1-2HH K2SF-GM1-2HPr K2SF-GM1-2HM
P-MK 1 K2SF-Mk1-2HH K2SF-Mk1-2HPr K2SF-Mk1-2HM
P-Tcell 1 NA K2SF-T1-2HPr K2SF-T1-2HM
P-Tcell 3 K2SF-T3-2HH NA NA
P-Bcell 1 NA K2SF-B1-2HPr K2SF-B1-2HM
P-Bcell 3 K2SF-B3-1H NA NA

 

SERUM-FREE, 4-PLATE ASSAY KITS

Cell Population Human Primate Mouse
None K2SF-0-4HH K2SF-0-4HPr K2SF-0-4HM
SC-HPP 2 K2SF-HPP2-4HH K2SF-HPP2-4HPr K2SF-HPP2-4HM
SC-GEMM 1 K2SF-GEMM1-4HH K2SF-GEMM1-4HPr K2SF-GEMM1-4HM
P-BFU 1 K2SF-BFU1-4HH K2SF-BFU1-4HPr K2SF-BFU1-4HM
P-GM 1 K2SF-GM1-4HH K2SF-GM1-4HPr K2SF-GM1-4HM
P-MK 1 K2SF-Mk1-4HH K2SF-Mk1-4HPr K2SF-Mk1-4HM
P-Tcell 1 NA K2SF-T1-4HPr K2SF-T1-4HM
P-Tcell 3 K2SF-T3-4HH NA NA
P-Bcell 1 NA K2SF-B1-4HPr K2SF-B1-4HM

 

SERUM-FREE,"GLOBAL" POPULATION ASSAY KITS

Cell Population Human Primate Mouse
"Global" 4-Pop K2SF-4P-5HH K2SF-4P-5PrH K2SF-4P-5MH
"Global" 5-Pop K2SF-5P-6HH K2SF-5P-6PrH K2SF-5P-6MH
"Global 7-Pop K2SF-7P-8HH K2SF-7P-8PrH K2SF-7P-8MH
For Research Use Only.

Luminescence or multimode plate reader with "glow" luminescence measuring capability.
  • HALO®-Tox HT Master Mix
  • ATP standard
  • ATP high and low controls
  • ATP Enumeration Reagent
  • Sterile, 96-well plates
  • Non-sterile, 96-well plates

 

Download the manual for HALO®-Tox HT
Download the Luminometer Setup and RLU to ATP Conversion Protocol
Download Certificate of Analysis (CoAs) for ATP bioluminescence components. Note: Some of CoAs will be included with the assay kits.
Download SDS for SEC Media for Kits
Download SDS for ATP Standard and Controls
Download SDS for ATP Enumeration Reagent