Hematopoietic Stem Cell Predictive Residual Toxicity and Sensitivity Assay
- Measure the proportion of primitive lympho-hematopoietic stem cells remaining or resistant to drug administration.
- Determine whether primitive lympho-hematopoietic stem cells change their sensitivity to a drug after a single dose.
- Measure the effect on primitive stem cells of multiple drug administration.
- Evaluate whether primitive lympho-hematopoietic stem cells exhibit expansion potential after a single drug dose.
- The only in vitro assay available that can detect whether primitive stem cells are available for potentially repopulating the lympho-hematopoietic system after a single drug dose.
- Indicates changes in drug sensitivity to primitive stem cells.
- 3R's alternative principle - Reduction, Refinement and Replacement for animal testing.
- Can be applied directly to fresh, primary human cells.
- Uses a 2-stage primitive stem cell "priming" and cell expansion in vitro re-plating technology.
- Incorporates Suspension Expansion Culture (SEC) and highly sensitive bioluminscence readout.
- Designed for multiplexing with other assays, including flow cytometry and gene expression analysis, to obtain the most information from a single sample.
- Highly cost effective and time efficient.
An example of an ATP standard curve and control results are shown in the diagram below and actual values for the kit being used are given in the Technical Manual. If the values obtained are within the ranges given in the Technical Manual, the user can process the samples knowing that the results will be trustworthy.
- Non-human primate
HALO® PRT is only for use with bone marrow.
HALO® PRT is available for the primitive lympho-hematopoietic stem cell populations:
- SC-HPP 1
- SC-HPP 2
Luminescence plate reader or multimode plate reader with "glow" luminescence measuring capability.
- HALO® PRT Master Mixes
- ATP standard
- ATP high and low controls
- ATP Enumeration Reagent
- 2 or 4 sterile, 96-well plates
- Non-sterile, 96-well plates
- Sterile, adhesive foil covers
HALO® PRT Assay Manual