CardioGlo™-Tox HT

Standardized and Validated ATP Bioluminescence Cardiotoxicity Assay Platform

 


Buy CardioGlo™-Tox HT Assay Kits
 

  • Cardiomyocytes have high intracellular concentrations of ATP. Changes in cardiomyocyte metabolism can be detected and measured using CardioGlo™-Tox HT.
  • Can be used with cardiomyocytes from different species as well as human ES or iPS-derived cardiomyocytes.
  • Specifically designed to screen and test for in vitro cardiotoxicity.
  • High throughput capability during in vitro ADME/Tox screening.
  • Allows drug and species ranking of toxicity.
  • Incorporates proven, standardized and validated ATP bioluminescence technology, the most sensitive signal detection system available.
  • Includes all reagents to calibrate and standardize the assay.
  • Measurement assurance parameters included to ensure the assay is working correctly.
  • Reliably compare results over time and between drug lots.
  • Assay kits include everything needed to perform the readout.
  • 96-Well plates included with all kits and available for adherent or non-adherent cells.
  • Available as 2- or 4-plate assay kits. Custom sizes also available.
  • Designed for multiplexing capability.
  • Rapid turnaround.
  • Simple and time efficient.

Principle of ATPBioluminescence Cell Proliferation/Cytotoxicity Assays

All ATP bioluminescence assays kits from Preferred Cell Systems™ include an ATP standard(s) and controls.  Performing the ATP standard curve and controls is highly recommended. The controls are used to calibrate the instrument and the ATP standard is used to standardize the assay. The values obtained provide the measurement assurance parameters that allow you, the user, to ensure that the results obtained can be trusted.

An example of an ATP standard curve and control results are shown in the diagram below and actual values for the kit being used are given in the Technical Manual. If the values obtained are within the ranges given in the Technical Manual, the user can process the samples knowing that the results will be trustworthy.

Calibration and standardization of an ATP bioluminescence assay from Preferred Cell Systems™ - Measurement Assurance
 

CardioGlo™-Tox HT can be used with cardiomyocytes from different species as well as human ES- or iPS-derived cardiomyocytes.

CardioGLO™-Tox HT assays are available with 96-well plates. Upon request, kits with 384-well plates are also available.

For Research Use Only. Not for clinical diagnostic use.
Luminescence or multimode plate reader with "glow" luminescence measuring capability.

 
  • IMDM base medium for dilution of ATP standard.
  • ATP standard.
  • ATP controls.
  • ATP Enumeration Reagent.
  • Sterile, solid white 96-well plates. Available for adherent or non-adherent cells.
  • Non-sterile, solid, white 96-well plates.
  • Sterile, adhesive foil covers to maintain sterility of unused wells.
CardioGlo™-Tox HT is flexible so that the investigator can use there own growth medium to culture the cells.
Download the CardioGlo™-Tox HT Technical Manual
Download the Luminometer Setup and RLU to ATP Conversion Procedure

When ordering CardioGlo™, simply click on Catalog Number and you will be taken directly to the desired product.

CardioGlo-Tox HT Assay Kits

Cell Type Included Catalog Number Assay Plate Type Number of Plates
Any KCG-T96A-2 Adherent 2
Any KCG-T96A-4 Adherent 4
Any KCG-T96NA-2 Non-Adherent 2
Any KCG-T96NA-4 Non-Adherent 4