ColonyGro™

Methylcellulose, Colony-Forming Cell (CFC) or Unit (CFU)
Reagents for Hematopoietic Stem and Progenitor Cells

 

Buy ColonyGro™ Reagents
 

Methylcellulose, colony-forming cell (CFC)/ unit (CFU) reagents for hematopoietic stem and progenitor cells from The Colony Experts.

 

  • All applications involving lympho-hematopoietic cells with a manual or image analysis readout.
  • Detect clonal ability of cells.
  • Evaluate the differentiation status or potential of different lympho-hematopoietic cell populations.
  • Correlate in vivo with in vitro differentiation.
  • Differentiation effects of growth factors or cytokines.
  • Evaluate ex vivo differential expansion.
  • Evaluate the effects of drugs, xenobiotics or other agents on the differentiation/maturation process.  
  • Superior performance compared to competitor reagents. 
  • Colonies can be counted after 9-10 days.
  • Setup 44 samples/100mL with ColonyGro™, instead of only 33 with reagents from other suppliers.
  • Available to detect 8 different stem cell populations, and 10 progenitor and precursor cell populations.
  • Unlike competitor formulations, ColonyGro™ CFC-GEMM media contains TPO, allowing the production of erythroid, granulocyte, macrophage and megakaryocytes to be produced as defined for the CFC-GEMM populations.
  • Same or lower price than MethoCult™ and other competitor reagents. 

Methylcellulose, Colony-Forming Cell (CFC) or Unit (CFU) Reagents for Hematopoietic Stem and Progenitor Cells
 
  • Human
  • Non-human primate (Macaca fascicularis (Cynomologus) and Macaca mulatta (Rhesus)
  • Dog
  • Rat
  • Mouse

The following cell populations are available for ColonyGro™. Those populations that have multiple growth factors will produce different colony types in the same culture. Please note that the number of colonies counted in culture containing multiple growth factors will be different to those with single growth factors, which only detect cells in a specific cell compartment. 

Cell Type Cell Population Detected (1) MethoCult™ Equivalent Growth Factor Cocktail Number of Samples(2)
Any None NE None 44
Stem cell HPP-SP 1, primitive stem cells NE 3,6,SCF,Flt3-L 44
Stem cell HPP-SP 2, and all lineages NE EPO,GM,2,3,6,7,SCF,TPO, Flt3-L 44
Stem cell CFC-GEMM 1, and all hematopoietic lineages NE EPO,GM,3,6,SCF,TPO, Flt3-L 44
Stem cell CFC-GEMM 2, and all hematopoietic lineages NE EPO,GM,3,6,SCF,TPO 44
Stem cell CFC-GEMM 3, and all hematopoietic lineages H4435 "Enriched"
MethoCult Express
EPO,GM,G,3,6,SCF TPO(3) 44
Stem cell CFC-GEM 1, and E and GM lineages only(4) NE EPO,GM3,6,SCF 44
Stem cell CFC-GEM 2, and E and GM lineages only H4034 "Optimum" EPO,GM,G,3,SCF 44
Stem cell CFC-GEM 3, and E and GM lineages only H4434 "Classic" EPO,GM,3,SCF 44
Progenitor BFU-E 1, primitive E cells NE EPO,3,SCF 44
Progenitor BFU-E 2, E cells only H4430 EPO (high dose) 44
Precursor CFU-E, mature E cells NE EPO (low dose) 44
Progenitor GM-CFC 1, primitive GM cells H4534 "Classic" GM,3,SCF 44
Progenitor GM-CFC 2, primitive GM and neutrophils H4035 "Optimum" GM,G,3,SCF 44
Progenitor GM-CFC 3, GM cells only NE GM alone 44
Precursor G-CFC, neutrophils only NE G alone 44
Precursor M-CFC, macrophages only NE M alone 44
Progenitor Mk-CFC 1, primitive megakryocytes NE TPO,3,SCF 44
Progenitor Mk-CFC 2, megakaryocytes only NE TPO alone 44
Progenitor B-CFC, B-lymphocytes NE 7 alone 44


NE = No equivalent product available.

(1) = Accepted nomenclature of cell populations detected by the colony-forming cell assay.

(2) = Number of samples that can be performed from 100mL by setting up a total volume of 2.5mL, including 10% cell volume), sufficient for duplicate 1mL cultures in 35mm Petri dishes.

(3) = Contains TPO to stimulate megakaryopoiesis. The equivalent MethoCult™ product does not contain TPO, and therefore cannot be designated as a CFC-GEMM stem cell population, but rather a CFC-GEM population.

(4) = E for erythropoietic; GM for granulocyte-macrophage.

When ordering ColonyGro™, please copy the Catalog Number, click on Catalog Number Search and paste the number, followed by Search. You will be taken directly to the desired product.

 

 

Cell Population Human Primate Dog Rat Mouse
None CG-0-100H CG-0-100Pr CG-0-100C CG-0-100R CG-0-100M
HPP-SP1 CG-HPP1-100H CG-HPP1-100Pr CG-HPP1-100C CG-HPP1-100R CG-HPP1-100M
HPP-SP2 CG-HPP2-100H CG-HPP2-100Pr CG-HPP2-100C CG-HPP2-100R CG-HPP2-100M
CFC-GEMM1 CG-GEMM1-100H CG-GEMM1-100Pr CG-GEMM1-100C CG-GEMM1-100R CG-GEMM1-100M
CFC-GEMM2 CG-GEMM2-100H CG-GEMM2-100Pr CG-GEMM2-100C CG-GEMM2-100R CG-GEMM2-100M
CFC-GEMM3 CG-GEMM3-100H NA NA NA NA
CFC-GEM1 CG-GEM1-100H CG-GEM1-100Pr CG-GEM1-100C CG-GEM1-100R CG-GEM1-100M
CFC-GEM2 CG-GEM2-100H CG-GEM2-100Pr CG-GEM2-100C CG-GEM2-100R CG-GEM2-100M
CFC-GEM3 CG-GEM3-100H NA NA NA NA
BFU-E1 CG-BFU1-100H CG-BFU1-100Pr CG-BFU1-100C CG-BFU1-100R CG-BFU1-100M
BFU-E2 CG-BFU2-100H CG-BFU2-100Pr CG-BFU2-100C CG-BFU2-100R CG-BFU2-100M
CFU-E CG-E-100H CG-E-100Pr CG-E-100C CG-E-100R CG-E-100M
GM-CFC1 CG-GM1-100H CG-GM1-100Pr CG-GM1-100C CG-GM1-100R CG-GM1-100M
GM-CFC2 CG-GM2-100H NA NA NA NA
GM-CFC3 CG-GM3-100H CG-GM3-100Pr CG-GM3-100C CG-GM3-100R CG-GM3-100M
G-CFC CG-G-100H CG-G-100Pr CG-G-100C CG-G-100R CG-G-100M
M-CFC CG-M-100H CG-M-100Pr CG-M-100C CG-M-100R CG-M-100M
MK-CFC1 CG-Mk1-100H CG-Mk1-100Pr CG-Mk1-100C CG-Mk1-100R CG-Mk1-100M
MK-CFC2 CG-Mk2-100H CG-Mk2-100Pr CG-Mk2-100C CG-Mk2-100R CG-Mk2-100M
B-CFC CG-B-100H CG-B-100Pr CG-B-100C CG-B-100R CG-B-100M

 

Cell Population Horse Pig Sheep
None CG-0-100E CG-0-100P CG-0-100O
HPP-SP1 CG-HPP1-100E CG-HPP1-100P CG-HPP1-100O
HPP-SP2 CG-HPP2-100E CG-HPP2-100P CG-HPP2-100O
CFC-GEMM1 CG-GEMM1-100E CG-GEMM1-100P CG-GEMM1-100O
CFC-GEMM2 CG-GEMM2-100E CG-GEMM2-100P CG-GEMM2-100O
CFC-GEMM3 NA NA NAO
CFC-GEM1 CG-GEM1-100E CG-GEM1-100P CG-GEM1-100O
CFC-GEM2 CG-GEM2-100E CG-GEM2-100P CG-GEM2-100O
CFC-GEM3 NA NA NA
BFU-E1 CG-BFU1-100E CG-BFU1-100P CG-BFU1-100O
BFU-E2 CG-BFU2-100E CG-BFU2-100P CG-BFU2-100O
CFU-E CG-E-100E CG-E-100P CG-E-100O
GM-CFC1 CG-GM1-100E CG-GM1-100P CG-GM1-100O
GM-CFC2 NA NA NA
GM-CFC3 CG-GM3-100E CG-GM3-100P CG-GM3-100O
G-CFC CG-G-100E CG-G-100P CG-G-100O
M-CFC CG-M-100E CG-M-100P CG-M-100O
MK-CFC1 CG-Mk1-100E CG-Mk1-100P CG-Mk1-100O
MK-CFC2 CG-Mk2-100E CG-Mk2-100P CG-Mk2-100O
B-CFC CG-B-100E CG-B-100P CG-B-100O

How ColonyGro for CFC-GEMM compares to MethoCult H4435  

Click image for larger view

The graph to the left shows a cell dose response comparison between ColonyGro™ for the primitive hematopoietic stem cell population, CFC-GEMM 1, and the nearest MethoCult™ equivalent population detected with H4435 media. The difference between the growth factors is given in the table above, but whereas the CFC-GEMM 1 growth factor cocktail contains Flt3-Ligand and thrombopoietin (TPO), to induce the production of megakaryocytes, MethoCult™ H4435, contains neither of these components. Consequently, MethoCult™ H4435 cannot produce megakaryocytes, and therefore does not stimulate the production of primitive stem cells and cannot be designated as a CFC-GEMM population; MethoCult™ H4435 actually stimulates a more mature stem cell population.

  • 100mL of ColonyGro™ Reagent
  • Sufficient for 44 duplicate samples
  • 2.5ml total volume prepared/sample
  • 0.25ml sample cell suspension volume required
  • 1ml plated in 2 x 35mm Petri dishes

For Research Use Only. Not for clinical diagnostic use.

Inverted microscope with a magnification of 40x - 100x.

Download the ColonyGro™ Manual
Certificate of Analysis (CoA) for each reagent type will be provided with shipment
Download methylcellulose SDS Sheet