CAMEO™-4 

Miniaturized, Methylcellulose Colony-Forming Cell (CFC) or Unit (CFU) 
Complete Assay Kit for Hematopoietic Stem and Progenitor Cells

Buy CAMEO™-4 Assay Kits
 

 

Miniaturized, methylcellulose colony-forming cell (CFC) / unit (CFU) complete assay kit for hematopoietic stem and progenitor cells from The Colony Experts.

 

  • Hematopoietic research and cellular therapy applications requiring a methylcellulose, clonal assay.
  • Evaluate differentiation status or potential of different lympho-hematopoietic cell populations.
  • Correlate in vivo with in vitro differentiation.
  • Differentiation effects of growth factors or cytokines.
  • Evaluate ex vivo differential expansion.
  • Evaluate the effects of drugs, xenobiotics or other agents on the differentiation/maturation process. 
  • Miniaturized assay format requires 1/10 of the volume of reagents, target cells and other sample requirements compared to traditional 35mm Petri dish CFC assays.
  • Greater number of replicates (4) improves statistics.
  • Smaller growth surface for more accurate and faster colony counting.
  • No meniscus; allows evaluation of colonies to the well edge.
  • Colonies counted after 9-10 days, compared to 14 days for MethoCult™ reagents.
  • CAMEO™-4 assay kits contain sufficient reagents and culture plates for 50 samples.
  • Complete flexibility; evaluate new growth factors and/or cytokines.
  • Available for 17 different cell populations and 8 different species.
  • Quality controlled media and superior performance compared to MethoCult™.

Miniaturized, Methylcellulose Colony-Forming Cell (CFC) or Unit (CFU)  Complete Assay Kit for Hematopoietic Stem and Progenitor Cells
 
  • Human
  • Non-human primate (Macaca fascicularis (Cynomologus) and Macaca mulatta (Rhesus))
  • Horse
  • Pig
  • Sheep
  • Dog
  • Rat
  • Mouse
Cell Type Cell Population Detected (1) MethoCult™ Equivalent Growth Factor Cocktail Number of Samples(2)
Any None NE None 50
Stem cell HPP-SP 1 NE 3,6,SCF,Flt3-L 50
Stem cell HPP-SP 2 NE EPO,GM,2,3,6,7,SCF,TPO, Flt3-L 50
Stem cell CFC-GEMM 1 NE EPO,GM,3,6,SCF,TPO, Flt3-L 50
Stem cell CFC-GEMM 2 NE EPO,GM,3,6,SCF,TPO 50
Stem cell CFC-GEMM 3 H4435 "Enriched"
MethoCult Express
EPO,GM,G,3,6,SCF TPO(3) 50
Stem cell CFC-GEM 1 NE EPO,GM3,6,SCF 50
Stem cell CFC-GEM 2 H4034 "Optimum" EPO,GM,G,3,SCF 50
Stem cell CFC-GEM 3 H4434 "Classic" EPO,GM,3,SCF 50
Progenitor BFU-E 1 NE EPO,3,SCF 50
Progenitor BFU-E 2 H4430 EPO (high dose) 50
Precursor CFU-E NE EPO (low dose) 50
Progenitor GM-CFC 1 H4534 "Classic" GM,3,SCF 50
Progenitor GM-CFC 2 H4035 "Optimum" GM,G,3,SCF 50
Progenitor GM-CFC 3 NE GM alone 50
Precursor G-CFC NE G alone 50
Precursor M-CFC NE M alone 50
Progenitor Mk-CFC 1 NE TPO,3,SCF 50
Progenitor Mk-CFC 2 NE TPO alone 50
Progenitor B-CFC NE 7 alone 50


NE = No equivalent product available.

(1) = Accepted nomenclature of cell populations detected by the colony-forming cell assay.

(2) = Number of samples that can be performed from 38mL by setting up a total volume of 0.6mL, including 10% cell volume), sufficient for quadruplicate 0.1mL cultures in 4-well, 35mm Petri dishes.

(3) = Contains TPO to stimulate megakaryopoiesis. The equivalent MethoCult™ product does not contain TPO and therefore cannot be designated as a CFC-GEMM stem cell population, but rather a CFC-GEM population.

 

How to count colonies in the CFU assay using the CAMEO-4 assay kit 

Click image for larger view

ColonyGro™ and CAMEO™-4 Incorporate the Same Growth Factor Cocktails

While ColonyGro™ uses 35mm Petri dishes, CAMEO™-4 is miniaturized using 4-wells in a 35mm Petri dish that only requires 0.1mL/well of reagent rather then 1mL of reagent. Therefore, the growth surface area for CAMEO™-4 is much smaller. As a result, the increased cell interaction allows the cells to grow faster, producing colonies in a shorter period of time. This graph shows a direct correlation between ColonyGro™ and CAMEO™-4.

When ordering CAMEO™-4, please copy the Catalog Number, click on Catalog Number Search and paste the number, followed by Search. You will be taken directly to the desired product.

 

Cell Population Human Primate Dog Rat Mouse
None KC-0-50H KC-0-50Pr KC-0-50C KC-0-50R KC-0-50M
HPP-SP1 KC-HPP1-50H KC-HPP1-50Pr KC-HPP1-50C KC-HPP1-50R KC-HPP1-50M
HPP-SP2 KC-HPP2-50H KC-HPP2-50Pr KC-HPP2-50C KC-HPP2-50R KC-HPP2-50M
CFC-GEMM1 KC-GEMM1-50H KC-GEMM1-50Pr KC-GEMM1-50C KC-GEMM1-50R KC-GEMM1-50M
CFC-GEMM2 KC-GEMM2-50H KC-GEMM2-50Pr KC-GEMM2-50C KC-GEMM2-50R KC-GEMM2-50M
CFC-GEMM3 KC-GEMM3-50H NA NA NA NA
CFC-GEM1 KC-GEM1-50H KC-GEM1-50Pr KC-GEM1-50C KC-GEM1-50R KC-GEM1-50M
CFC-GEM2 KC-GEM2-50H KC-GEM2-50Pr KC-GEM2-50C KC-GEM2-50R KC-GEM2-50M
CFC-GEM3 KC-GEM3-50H NA NA NA NA
BFU-E1 KC-BFU1-50H KC-BFU1-50Pr KC-BFU1-50C KC-BFU1-50R KC-BFU1-50M
BFU-E2 KC-BFU2-50H KC-BFU2-50Pr KC-BFU2-50C KC-BFU2-50R KC-BFU2-50M
CFU-E KC-E-50H KC-E-50Pr KC-E-50C KC-E-50R KC-E-50M
GM-CFC1 KC-GM1-50H KC-GM1-50Pr KC-GM1-50C KC-GM1-50R KC-GM1-50M
GM-CFC2 CG-GM2-100H NA NA NA NA
GM-CFC3 KC-GM3-50H KC-GM3-50Pr KC-GM3-50C KC-GM3-50R KC-GM3-50M
G-CFC KC-G-50H KC-G-50Pr KC-G-50C KC-G-50R KC-G-50M
M-CFC KC-M-50H KC-M-50Pr KC-M-50C KC-M-50R KC-M-50M
MK-CFC1 KC-Mk1-50H KC-Mk1-50Pr KC-Mk1-50C KC-Mk1-50R KC-Mk1-50M
MK-CFC2 KC-Mk2-50H KC-Mk2-50Pr KC-Mk2-50C KC-Mk2-50R KC-Mk2-50M
B-CFC KC-B-50H KC-B-50Pr NA NA KC-B-50M

 

Cell Population Horse Pig Sheep
None KC-0-50E KC-0-50P KC-0-50O
HPP-SP1 KC-HPP1-50E KC-HPP1-50P KC-HPP1-50O
HPP-SP2 KC-HPP2-50E KC-HPP2-50P KC-HPP2-50O
CFC-GEMM1 KC-GEMM1-50E KC-GEMM1-50P KC-GEMM1-50O
CFC-GEMM2 KC-GEMM2-50E KC-GEMM2-50P KC-GEMM2-50O
CFC-GEMM3 NA NA NA
CFC-GEM1 KC-GEM1-50E KC-GEM1-50P KC-GEM1-50O
CFC-GEM2 KC-GEM2-50E KC-GEM2-50P KC-GEM2-50O
CFC-GEM3 NA NA NA
BFU-E1 KC-BFU1-50E KC-BFU1-50P KC-BFU1-50O
BFU-E2 KC-BFU2-50E KC-BFU2-50P KC-BFU2-50O
CFU-E KC-E-50E KC-E-50P KC-E-50O
GM-CFC1 KC-GM1-50E KC-GM1-50P KC-GM1-50O
GM-CFC2 NA NA NA
GM-CFC3 KC-GM3-50E KC-GM3-50P KC-GM3-50O
G-CFC KC-G-50E KC-G-50P KC-G-50O
M-CFC KC-M-50E KC-M-50P KC-M-50O
MK-CFC1 KC-Mk1-50E KC-Mk1-50P KC-Mk1-50O
MK-CFC2 KC-Mk2-50E KC-Mk2-50P KC-Mk2-50O
For Research Use Only. Not for clinical diagnostic use.

Inverted microscope with a magnification of 40x - 100x.

  • CAMEO™-4 reagent. Choose specific cell population and species.
  • 50 x 4-well, 35mm cell culture plates.

QuickGuide to CAMEO™-4, Miniaturized Complete Methylcellulose Colony-Forming Unit (CFU)  Assay Kit

Download the CAMEO™-4 Manual
Download the SDS Sheet