• Detect multiple stem cell and multi-lineage colonies in a single culture.
• Any type of hematopoietic research requiring clonal growth of proliferating cell populations.
• Any hematopoietic cellular therapy application requiring the simultaneous enumeration of multiple cell types in vitro

• Includes a proprietary growth factor cocktail that allows the production of different hematopoietic stem cell colonies, (CFC-GEMM and CFC-GEM) as well as multiple progenitor cell colonies (BFU-E, CFU-E, GM-CFC, M-CFC, G-CFC, Mk-CFC and even T-CFC and B-CFC). PLEASE NOTE: The formation of colonies from different lineages depends on the source, status and degree of fractionation of the cell suspension used.
• Available in 3 different culture formats (see below).
• Colonies can be enumerated between day 5 and 12 of culture depending on species and tissue.
• Colony cells can be removed from the plate and prepared for flow cytometric phenotypic analysis.
• The proprietary growth factor and supplement cocktail used allows a greater diversity of colonies to be produced than any other competitor product.

• AllColonies™ Traditional: 35mm Petri dish format, used for all colony-forming cell (CFC) / (CFU) assays. Sufficient reagent for 44 samples.
• AllColonies™ Micro: Miniaturized, 4-well assay that includes 50 culture plates for 50 samples.
• AllColonies™ Multi: A complete 96-well plate format that allows manual enumeration of colonies and measurement of total cell colony proliferation to standardize the AllColonies™ assay using an ATP bioluminescence readout.

 

The following are a series of colony images from cultures using the AllColonies™ Reagent. AllColonies™ produces massive colonies that are derived from very primitive lympho-hematopoietic stem cells stimulated with a proprietary cocktails of factors. The colonies produced demonstrate that the cells can be from individual lineages or multiple lineages. The size of some of the colonies is an indication of the primitiveness of the cell giving rise to the colony.

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Image 10: How many colonies are in this image?

• Human
• Non-human primate
• Dog
• Rat
• Mouse

• Bone marrow from all species
• Umbilical cord blood from human tissue
• Peripheral blood from all species
• Mobilized peripheral blood from human tissue
• Other hematopoietic organs and tissues from different species

AllColonies™ Traditional: 35mm Plate Format

• 100mL AllColonies™ Reagent

AllColonies™ Micro: Miniaturized Format

• AllColonies™ Reagent
• 50 culture plates

AllColonies™ Multi: 96-Well Plate Format

• AllColonies™ Reagent
• Sterile 96-well plate for cell culture
• Non-sterile 96-well plate for assay standardization
• ATP Enumeration Reagent
• ATP standard
• ATP controls
• Sterile adhesive foil to maintain unused wells sterile

For Research Use Only.

An inverted microscope with 40x and at least 100x magnification is required for all three assay formats.
A luminescence plate reader is also required for the 96-well plate format assay.

 

Download the AllColonies™ Traditional Manual
Download the AllColonies™ Micro Manual
Download the AllColonies™ Multi Manual
Download the Document for Luminometer Setup and RLU to ATP Conversion
Download Certificate of Analysis (CoAs) for ATP Bioluminescence Components. Note: Some CoAs will be included with assay kit
Download the SDS sheet

When ordering AllColonies™, you can quickly find the product by copying the catalog number in the table below and using it in the catalog search function.

Assay	Catalog No.	Enumeration	Format	Species	No. of Samples
Reagent	AL-CG-100C	10	35mm Petri Dish	Dog
Reagent	AL-CG-100H	03	35mm Petri Dish	Human
Reagent	AL-CG-100M	09	35mm Petri Dish	Mouse
Reagent	AL-CG-100Pr	04	35mm Petri Dish	Primate
Reagent	AL-CG-100R	08	35mm Petri Dish	Rat
Assay Kit	AL-KCO-1C	GMP grade	1 x 96-well plate	Dog
Assay Kit	AL-KCO-1H	Human	1 x 96-well plate	Human	6
Assay Kit	AL-KCO-1M	GMP Fibrinogen-free	1 x 96-well plate	Mouse
Assay Kit	AL-KCO-1Pr	Research	1 x 96-well plate	Primate
Assay Kit	AL-KCO-1R	GMP Quarantine	1 x 96-well plate	Rat
Assay Kit	AL-KC-50C	Multiple	Miniaturized 50 culture dishes	Dog	3
Assay Kit	AL-KC-50H	Multiple	Miniaturized 50 culture dishes	Human	1
Assay Kit	AL-KC-50M	Human	Miniaturized 50 culture dishes	Mouse	5
Assay Kit	AL-KC-50Pr	Multiple	Miniaturized 50 culture dishes	Primate	2
Assay Kit	AL-KC-50R	Multiple	Miniaturized 50 culture dishes	Rat	4