AllColonies™ Micro: Miniaturized, 4-well assay that includes 50 culture plates for 50 samples.

 

AllColonies™

A Multi-Stem Cell, Multi-Lineage, Hematopoietic Minaturized Methylcellulose CFU Assay

Cell Population	Species	Formulation	Catalog Number	Quantity
Multi-Stem Cells
Multi-Lineage	Human	Low serum	AL-KC-50H	50mL
Multi-Lineage	Mouse	Low serum	AL-KC-50M	50mL
Multi-Lineage	Primate	Low serum	AL-KC-50Pr	50mL
Multi-Lineage	Rat	Low serum	AL-KC-50R	50mL
Multi-Lineage	Dog	Low serum	AL-KC-50C	50mL

AllColonies™ Traditional: 35mm Petri dish format, used for all colony-forming unit (CFU) assays.
Sufficient reagent for 44 samples.

AllColonies™

A Multi-Stem Cell, Multi-Lineage, Hematopoietic Minaturized Methylcellulose CFU Assay

Cell Population	Species	Formulation	Catalog Number	Quantity
Multi-Stem Cells
Multi-Lineage	Human	Low serum	AL-CG-100H	100mL
Multi-Lineage	Mouse	Low serum	AL-CG-100M	100mL
Multi-Lineage	Primate	Low serum	AL-CG-100Pr	100mL
Multi-Lineage	Rat	Low serum	AL-CG-100R	100mL
Multi-Lineage	Dog	Low serum	AL-CG-100C	100mL

AllColonies™ Multi: A complete assay kit using a 96-well plate format that allows manual enumeration of colonies and measurement of total cell colony proliferation to standardize the AllColonies™ assay using an ATP bioluminescence readout.

 

AllColonies™

A Multi-Stem Cell, Multi-Lineage, Hematopoietic Traditional Methylcellulose CFU Assay

Cell Population	Species	Formulation	Catalog Number	Quantity
Multi-Stem Cells
Multi-Lineage	Human	Low serum	AL-KCO-1H	1 Kit
Multi-Lineage	Mouse	Low serum	AL-KCO-1M	1 Kit
Multi-Lineage	Primate	Low serum	AL-KCO-1Pr	1 Kit
Multi-Lineage	Rat	Low serum	AL-KCO-1R	1 Kit
Multi-Lineage	Dog	Low serum	AL-KCO-1C	1 Kit

• Identify primitive lympho-hematopoietic stem cells.
• Study the presence and functional potential and ability of primitive stem cells.
• Identify different stem cell populations and their differentiated progeny potential in a single culture.
• Any type of hematopoietic research requiring clonal growth of proliferating cell populations.
• AllColonies™ can also be used, in a limited capacity, to identify the presence of primitive stem cells in a hematopoietic cellular therapy product.

• Includes a proprietary growth factor cocktail that allows the production of different hematopoietic stem cell colonies, (CFC-GEMM and CFC-GEM) as well as multiple progenitor cell colonies (BFU-E, CFU-E, GM-CFC, M-CFC, G-CFC, Mk-CFC and even T-CFC and B-CFC). PLEASE NOTE: The formation of colonies from different lineages depends on the source, status and degree of fractionation of the cell suspension used.
• Available in 3 different culture formats (see below).
• Colonies can be enumerated between day 5 and 12 of culture depending on species and tissue.
• Colony cells can be removed from the plate and prepared for flow cytometric phenotypic analysis.
• The proprietary growth factor and supplement cocktail used allows a greater diversity of colonies to be produced than any other competitor product.

The following are a series of colony images from cultures using the AllColonies™ Reagent. AllColonies™ produces massive colonies that are derived from very primitive lympho-hematopoietic stem cells stimulated with a proprietary cocktails of factors. The colonies produced demonstrate that the cells can be from individual lineages or multiple lineages. The size of some of the colonies is an indication of the primitiveness of the cell giving rise to the colony.

Image 1

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Image 4


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Image 10: How many colonies are in this image?

• Human
• Non-human primate
• Dog
• Rat
• Mouse

• Bone marrow from all species
• Umbilical cord blood from human tissue
• Peripheral blood from all species
• Mobilized peripheral blood from human tissue
• Other hematopoietic organs and tissues from different species

Preferred Cell Systems™ provides 4 different colony-forming unit assays in 3 different formats. 

AllColonies™ is one of the 4 CFU assays available in a 35mm Petri dish format. Below, it is compared to the other 3 CFU assays available from Preferred Cell Systems™.

Characteristics	AllColonies™: Traditional/ Micro/Multi	ColonyGro™	CAMEO™-4	CAMEO™-96
Uses:	Basic and stem cell research. Veterinary research. Limited cellular therapy.	Basic and stem cell research. Veterinary research. Limited cellular therapy.	Basic and stem cell research. Veterinary research. Limited cellular therapy.	Basic and stem cell research. Veterinary research. Limited cellular therapy. Limited hematotoxicity testing
Assay availability:	100mL/50mL/1kit	100mL	50mL	1 kit
Number of samples:	44/50/Depends on number of replicates required(1)	44	50	Depends on number of replicates required(1)
Type of assay:	Methylcellulose  - Clonal	Methylcellulose - Clonal	Methylcellulose - Clonal	Methylcellulose - Clonal
Assay format:	35mm Petri dish/ 4-well Petri dish/ 96-well plate	35mm Petri dish	4-well Petri dish	96-well plate
Cell functionality detected:	Differentiation or cell differentiation AND proliferation	Differentiation	Differentiation	Cell differentiation AND proliferation
Readout:	Microscopic or microscopic AND plate reader	Microscopic	Microscopic	Microscopic AND plate reader
Readout endpoint:	Colony counts or colony counts WITH ATP bioluminescence	Colony counts	Colony counts	Colony counts WITH ATP bioluminescence
Subjectivity:	Subjective/subjective/ non-subjective	Subjective	Subjective	Subjective AND non-subjective
Cell types detected:	Primitive lympho- hematopoietic stem cells	Stem, progenitor and/or precursor cells	Stem, progenitor and/or precursor cells	Stem and/or progenitor cells
Species:	Human. Primate. Dog. Rat. Mouse	Human. primate. Horse. Pig. Sheep. Dog. rat. Mouse	Human. primate. Horse. Pig. Sheep. Dog. rat. Mouse	Human. primate. Horse. Pig. Sheep. Dog. rat. Mouse
MethoCult™ equivalent assays available:	No	Yes	Yes	Yes
Total culture volume required:	2.5mL/0.6mL/ Depends on replicate number (2)	2.5mL	0.6mL	Depends on replicate number (2)
Number of replicates/culture:	2/4/As many as required	2	4	As many as required
Volume/culture:	1mL/0.1mL/0.1mL	1mL	0.1mL	0.1mL
Cell culture time(3):	6-10 days	2-12 days	2-9 days	4-9 days
Instrument calibration:	Only for AllColonies™ Multi(4)	No	No	Yes(4)
Standardized assay (5):	Only for AllColonies™ Multi	No	No	Yes
Proficiency testing incorporated into assay(6):	Only for AllColonies™ Multi	No	No	Yes
Measurement assurance(7):	Only for AllColonies™ Multi	No	No	Yes
Validation(8):	Only for AllColonies™ Multi	No	No	Yes

(1) = The number of samples tested depends on the number of replicates/sample performed. For example, if 6 replicates/sample are performed, then 16 samples can be analyzed on a single 96-well plate. These samples, however, do not have to performed on the same day.
(2) = The total culture volume for AllColonies™ Multi and CAMEO™-96 depends on the number of replicates/sample performed. If 4 replicates are required, 0.6mL total volume would be prepared, while for 6 replicates, 0.8mL would be prepared.
(3) = Culture time depends on the the state of fractionation or purification of the source cells, cell population tested and species.
(4) = Instrument calibration is performed using the ATP controls included with the assay kit.
(5) = Standardization implies performing a standard curve. ATP standards are included. ColonyGro™ and CAMEO™-4 or any other methylcellulose colony-forming assay from competitors do not include standards or controls and can not be standardized. "Standardized media" does not make an assay "standardized". 
6 = Since AllColonies™ Multi and CAMEO™-96 include standards and controls that allow the results to be compared to measurement assurance parameters every time an assay is performed, proficiency testing is also performed automatically at the same time as the instrument is calibrated and the assay standardized.
7 = Measurement assurance parameters are included in the Instruction Manual.
8 = Validation parameters are included in the Instruction Manual. Since standards and controls are included with these assays, validation can be performed in-house. ColonyGro™ and CAMEO™-4 as well as all other methylcellulose colony-forming assays from competitors, do not include standards and controls and, therefore, cannot be standardized or validated.

AllColonies™ Traditional: 35mm Plate Format

• 100mL AllColonies™ Reagent

AllColonies™ Micro: Miniaturized Format

• AllColonies™ Reagent
• 50 culture plates

AllColonies™ Multi: 96-Well Plate Format

• AllColonies™ Reagent
• Sterile 96-well plate for cell culture
• Non-sterile 96-well plate for assay standardization
• ATP Enumeration Reagent
• ATP standard
• ATP controls
• Sterile adhesive foil to maintain unused wells sterile

For Research Use Only.

An inverted microscope with 40x and at least 100x magnification is required for all three assay formats.
A luminescence plate reader is also required for the 96-well plate format assay.

 

Download the AllColonies™ Traditional Manual
Download the AllColonies™ Micro Manual
Download the AllColonies™ Multi Manual
Download the Document for Luminometer Setup and RLU to ATP Conversion
Download Certificate of Analysis (CoAs) for ATP Bioluminescence Components. Note: Some CoAs will be included with assay kit
Download the SDS sheet