AllColonies™
A Multi-Stem Cell, Multi-Lineage, Hematopoietic Methylcellulose Colony-Forming Cell
or Unit (CFC/CFU) Assay, Available in 3 Formats for Research and Cellular Therapy
Buy AllColonies Reagent & Kits

Uses
- Detect multiple stem cell and multi-lineage colonies in a single culture.
- Any type of hematopoietic research requiring clonal growth of proliferating cell populations.
- Any hematopoietic cellular therapy application requiring the simultaneous enumeration of multiple cell types in vitro
- Includes a proprietary growth factor cocktail that allows the production of different hematopoietic stem cell colonies, (CFC-GEMM and CFC-GEM) as well as multiple progenitor cell colonies (BFU-E, CFU-E, GM-CFC, M-CFC, G-CFC, Mk-CFC and even T-CFC and B-CFC). PLEASE NOTE: The formation of colonies from different lineages depends on the source, status and degree of fractionation of the cell suspension used.
- Available in 3 different culture formats (see below).
- Colonies can be enumerated between day 5 and 12 of culture depending on species and tissue.
- Colony cells can be removed from the plate and prepared for flow cytometric phenotypic analysis.
- The proprietary growth factor and supplement cocktail used allows a greater diversity of colonies to be produced than any other competitor product.
- AllColonies™ Traditional: 35mm Petri dish format, used for all colony-forming cell (CFC) / (CFU) assays. Sufficient reagent for 44 samples.
- AllColonies™ Micro: Miniaturized, 4-well assay that includes 50 culture plates for 50 samples.
- AllColonies™ Multi: A complete 96-well plate format that allows manual enumeration of colonies and measurement of total cell colony proliferation to standardize the AllColonies™ assay using an ATP bioluminescence readout.
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Image 10: How many colonies are in this image?

- Human
- Non-human primate
- Dog
- Rat
- Mouse
- Bone marrow from all species
- Umbilical cord blood from human tissue
- Peripheral blood from all species
- Mobilized peripheral blood from human tissue
- Other hematopoietic organs and tissues from different species
AllColonies™ Traditional: 35mm Plate Format
- 100mL AllColonies™ Reagent
AllColonies™ Micro: Miniaturized Format
- AllColonies™ Reagent
- 50 culture plates
AllColonies™ Multi: 96-Well Plate Format
- AllColonies™ Reagent
- Sterile 96-well plate for cell culture
- Non-sterile 96-well plate for assay standardization
- ATP Enumeration Reagent
- ATP standard
- ATP controls
- Sterile adhesive foil to maintain unused wells sterile
An inverted microscope with 40x and at least 100x magnification is required for all three assay formats.
A luminescence plate reader is also required for the 96-well plate format assay.
Download the AllColonies™ Micro Manual
Download the AllColonies™ Multi Manual
Download the Document for Luminometer Setup and RLU to ATP Conversion
Download Certificate of Analysis (CoAs) for ATP Bioluminescence Components. Note: Some CoAs will be included with assay kit
Download the SDS sheet
When ordering AllColonies™, you can quickly find the product by copying the catalog number in the table below and using it in the catalog search function.
Assay | Catalog No. | Enumeration | Format | Species | No. of Samples |
---|---|---|---|---|---|
Reagent | AL-CG-100C | 10 | 35mm Petri Dish | Dog | |
Reagent | AL-CG-100H | 03 | 35mm Petri Dish | Human | |
Reagent | AL-CG-100M | 09 | 35mm Petri Dish | Mouse | |
Reagent | AL-CG-100Pr | 04 | 35mm Petri Dish | Primate | |
Reagent | AL-CG-100R | 08 | 35mm Petri Dish | Rat | |
Assay Kit | AL-KCO-1C | GMP grade | 1 x 96-well plate | Dog | |
Assay Kit | AL-KCO-1H | Human | 1 x 96-well plate | Human | 6 |
Assay Kit | AL-KCO-1M | GMP Fibrinogen-free | 1 x 96-well plate | Mouse | |
Assay Kit | AL-KCO-1Pr | Research | 1 x 96-well plate | Primate | |
Assay Kit | AL-KCO-1R | GMP Quarantine | 1 x 96-well plate | Rat | |
Assay Kit | AL-KC-50C | Multiple | Miniaturized 50 culture dishes |
Dog | 3 |
Assay Kit | AL-KC-50H | Multiple | Miniaturized 50 culture dishes |
Human | 1 |
Assay Kit | AL-KC-50M | Human | Miniaturized 50 culture dishes |
Mouse | 5 |
Assay Kit | AL-KC-50Pr | Multiple | Miniaturized 50 culture dishes |
Primate | 2 |
Assay Kit | AL-KC-50R | Multiple | Miniaturized 50 culture dishes |
Rat | 4 |