HemoFLUOR™ PMT

A Fast and Easy "Global" Fluorescence Assay to Monitor Lympho-Hematopoietic Reconstitution After Transplantation

Description	Tissue	Formulation	Catalog Number	Quantity
Multiple
4-Population + Background	Bone marrow or peripheral blood	Low serum	K5-4PMT-5	1 Kit
5-Population + Background	Bone marrow or peripheral blood	Low serum	K5-5PMT-6	1 Kit
4-Population + Background	Bone marrow or peripheral blood	Low serum	K5-7PMT-8	1 Kit
Multiple
4-Population + Background	Bone marrow or peripheral blood	Serum-Free	K5SF-4PMT-5	1 Kit
5-Population + Background	Bone marrow or peripheral blood	Serum-Free	K5SF-5PMT-6	1 Kit
7-Population + Background	Bone marrow or peripheral blood	Serum-Free	K5SF-7PMT-8	1 Kit

How to Perform a Fluorescence-Based Readout (for HemoFLUOR™ PMT "Global")
 

Determine "global" hematopoietic and lympho-hematopoietic stem and progenitor cell reconstitution in patients that have undergone stem cell transplantation.

• Replacement for the CFU assay for hematopoietic lineage reconstitution.

• Measurement of progenitor cell proliferation, rather than differentiation, provides faster results.
• Measures individual hematopoietic lineages providing a faster prediction of possible reconstitution than the €œclassic€ CFU assay.
• No colony counting required.
• Transforms the subjective CFU assay into a non-subjective, instrument-based, quantitative assay.
• Easier to use than any traditional CFU assay, even using automated colony counting.
• Complete assay kit; includes everything needed to culture and obtain results.
• Easy to learn, fast to use and cheaper than methylcellulose CFU assays.
• Incorporates proven Suspension Expansion Culture™ (SEC™) Technology.

 

• Incorporates the measurement of non-fluorescent Resazurin (alimaBlue™) to fluorescent resorufin in an oxidation/reduction reaction that occurs only in live cells. The amount of fluorescence (relative fluorescence units, RFU) is dependent on the metabolic status of the cells and therefore is not only a measure of metabolic viability, but cell proliferation or inhibition.
• After culture, add 10μL of reagent, mix and measure signal between 30 minutes and 4 hours in a fluorescence plate reader.
• Uses a plate fluorometer or multimode plate reader to measure fluorescence with an excitation filter 560nmand an emission filter of 590nm.

HemoFLUOR™ PMT "Global" Assay Kits are available as:

• 4-Population "Global" Assay consists of: SC-GEMM 1, P-BFU 1, P-GM 1, P-Mk 1 and Background
• 5-Population "Global" Assay consists of: SC-HPP 2, SC-GEMM 1, P-BFU 1, P-GM 1, P-Mk 1 and Background
• 7-Population "Global" Assay consists of: SC-HPP 2, SC-GEMM 1, P-BFU 1, P-GM 1, P-Mk 1, P-Tcell 3, P-Bcell 2 and Background

Each HemoFLUOR™ PMT "Global" Assay Kit contains 5, 6 or 8 x 96-well plates, one for each lineage and background. Each sample is meant to be assessed in 8 replicates, but can be reduced to 4 replicates. Therefore, if using 8 replicates/sample, 12 samples can be assessed using a single assay kit.

• Peripheral blood
• Bone marrow (biopsy)

For research use only. Not for clinical diagnostic use.

A fluorescence or multimode plate reader with an excitation filter at 560nm and an emission filter at 590nm.

• HemoFLUOR™ PMT "Global" Master Mixes for each cell population tested
• Resazurin Reagent
• 3% SDS to stop the reaction
• Resorufin to standardize the assay
• Non-sterile 96-well plate to standardize the assay
• Sterile, 96-well plate(s)
• Sterile, adhesive cover foils to maintain sterility of unused wells