HemoFLUOR™ TE

Predict Neutrophil, Platelet and/or Erythroid Engraftment in 5 Days

 

Download the Time to Engraftment Assay Flyer  

 

Buy HemoFLUOR™ TE Assay Kits
 

Fluorescence assays to determine time to engraftment of neutrophils, red blood cells and platelets after stem cell transplantation​
 

A specific video tutorial on using the TE assays is not yet ready. Please scroll down and check out the QuickGuide and Technical Manual (if available). Below, is the link to perform the fluorescence readout:

How to Perform a Fluorescence-Based Readout (for HemoFLUOR TE)
 

  • Rapidly predict and measure the time to neutrophil, erythroid and/or platelet engraftment after hematopoietic stem cell transplantation.
  • To promote "Best Practice Criteria Testing."
  • Uses a resazurin (alamarBlue™) reagent that is added in a single step to produce a pink/red fluorescence as the resazurin is converted to fluorescent resorufin (excitation filter, 560nm; emission filter 590nm in a fluorescence plate reader or multimode plate reader).
  • Measure the ability and amount of GM-CFC, BFU-E and/or Mk-CFC progenitor cell proliferation prior to detecting differentiated cells in a CFU assay.
  • Proliferation of progenitor cells can be detected within 5 days.
  • Allows for the detection of individual hematopoietic progenitor cell populations or all three simultaneously after transplantation.
  • Faster, easier and more accurate to use than any traditional CFC assay, even with automated colony counting.
  • Incorporates methylcellulose-free, Suspension Expansion Culture™ (SEC™) Technology.
  • Rapid, accurate and cost-effective.
  • Available with low serum or serum-free formulations.

Principle of HemoFLUOR™ TE to Measure Time to Engraftment Using a Fluorescence Readout
 
  • Umbilical cord blood
  • Peripheral blood
  • Bone marrow biopsy

Tissues used for Detection

  • Peripheral blood
  • Bone marrow biopsy

The recommended cell purity is a mononuclear cell (MNC) fraction. A total nucleated cell (TNC) fraction is not recommended as this contains high concentrations of cell impurities, such as red blood cells, neutrophils, platelets and other cells that severely underestimate and even inhibit the detection of progenitor cells. 

For Research Use Only. Not for clinical diagnostic use.

Fluorescence or multimode plate reader with an excitation filter of 560nm and an emission filter of 590nm.

  • HemoFLUOR™ TE Master Mix(s) as either low serum or serum-free formulations
  • Resazurin Reagent
  • 3% SDS to stop the reaction
  • Resorufin Reagent to standardize the assay
  • Sterile, 96-well plates
  • Non-sterile, 96-well plates to standardize the assay
  • Sterile, adhesive foil covers

The HemoFLUOR™ TE Assay Manual

How to Perform HemoFLUOR TE to Measure Time to Engraftment of Stem Cells Using a Fluorescence Readout

When ordering HemoFLUOR™ TE, simply click on the Catalog Number in the table below and you will be taken directly to the desired product.

To determine Time to Engraftment:

  • P-BFU 1: Erythropoietic progenitor cell, used to predict red blood cell engraftment.
  • P-GM 1: Granulocyte-macrophage progenitor cell, used to determine neutrophil engraftment.
  • P-Mk 1: Megkaryopoietic progenitor cell, used to predict platelet engraftment.

 

HemoFLUOR™ TE Assays Available
Population Measured Catalog
Number
Format Number of Plates/Kit Number of Samples/Kit
P-BFU 1 K5-BTE-1 Low serum 1 16-24(#)
P-BFU 1 K5SF-BTE-1 Serum-Free 1 16-24(#)
P-GM 1 K5-GMTE-1 Low serum 1 16-24(#)
P-GM 1 K5SF-GMTE-1 Serum-Free 1 16-24(#)
P-Mk 1 K5-MkTE-1 Low serum 1 16-24(#)
P-Mk 1 K5SF-MkTE-1 Serum-Free 1 16-24(#)
P-BFU 1 and P-GM 1 and P-Mk 1 K5-3PTE-3 Low serum 3 16-24(#)
P-BFU 1 and P-GM 1 and P-Mk 1 K5SF-3PTE-3 Serum-Free 3 16-24(#)

(#): These assay kits can detect 16-24 samples for each cell population, using 4-6 replicates/sample.