HemoFLUOR™ TE

The Fast and Easy Fluorescence Assay to Predict Neutrophil, Platelet and/or Erythroid Engraftment After Stem Cell Transplantation

 

 

HemoFLUOR™ TE

A Fast and Easy Fluorescence Assay to Predict Neutrophil, Platelet and/or Erythroid Engraftment

Description Tissue Formulation Catalog Number Quantity
Lineage-Specific Cells
P-BFU 1 Bone marrow or peripheral blood Low serum K5-BTE-1 1 Kit
P-GM 1 Bone marrow or peripheral blood Low serum K5-GMTE-1 1 Kit
P-Mk 1 Bone marrow or peripheral blood Low serum K5-MkTE-1 1 Kit
P-BFU 1 + P-GM 1 + P-Mk 1 Bone marrow or peripheral blood Low serum K5-3PTE-3 1 Kit
Lineage-Specific Cells
P-BFU 1 Bone marrow or peripheral blood Serum-Free K5SF-BTE-1 1 Kit
P-GM 1 Bone marrow or peripheral blood Serum-Free K5SF-GMTE-1 1 Kit
P-Mk 1 Bone marrow or peripheral blood Serum-Free K5SF-MkTE-1 1 Kit
P-BFU 1 + P-GM 1 + P-Mk 1 Bone marrow or peripheral blood Serum-Free K5SF-3PTE-3 1 Kit

 

A fluorescence assay to predict and monitor time to engraftment after stem cell transplantation
 

Download the Time to Engraftment Assay Flyer  

 

A specific video tutorial on using the TE assays is not yet ready. Please scroll down and check out the QuickGuide and Technical Manual (if available). Below, is the link to perform the fluorescence readout:

How to Perform a Fluorescence-Based Readout (for HemoFLUOR TE)
 

  • Uses a resazurin (alamarBlue™) reagent that is added in a single step to produce a pink/red fluorescence as the resazurin is converted to fluorescent resorufin (excitation filter, 560nm; emission filter 590nm in a fluorescence plate reader or multimode plate reader).
  • Measure the ability and amount of GM-CFC, BFU-E and/or Mk-CFC progenitor cell proliferation prior to detecting differentiated cells in a CFU assay.
  • Proliferation of progenitor cells can be detected within 5 days.
  • Allows for the detection of individual hematopoietic progenitor cell populations or all three simultaneously after transplantation.
  • Faster, easier and more accurate to use than any traditional CFC assay, even with automated colony counting.
  • Incorporates methylcellulose-free, Suspension Expansion Culture™ (SEC™) Technology.
  • Rapid, accurate and cost-effective.
  • Available with low serum or serum-free formulations.

Principle of HemoFLUOR™ TE to Measure Time to Engraftment Using a Fluorescence Readout
 
The following assays are available to monitor Time to Engraftment:
  • P-BFU 1 for erythroid engraftment.
  • P-GM 1 for neutrophil engraftment
  • P-Mk 1 for platelet engraftment
  • P-BFU 1 + P-GM 1 + P Mk 1 for multilineage engraftment
Each assay can monitor a maximum of 24 samples at 4 replicates each. Higher numbers of replicates can also be used.
  • Umbilical cord blood
  • Peripheral blood
  • Bone marrow biopsy

Tissues used for Detection

  • Peripheral blood
  • Bone marrow biopsy

The recommended cell purity is a mononuclear cell (MNC) fraction. A total nucleated cell (TNC) fraction is not recommended as this contains high concentrations of cell impurities, such as red blood cells, neutrophils, platelets and other cells that severely underestimate and even inhibit the detection of progenitor cells. 

For Research Use Only. Not for clinical diagnostic use.

Fluorescence or multimode plate reader with an excitation filter of 560nm and an emission filter of 590nm.

  • HemoFLUOR™ TE Master Mix(s) as either low serum or serum-free formulations
  • Resazurin Reagent
  • 3% SDS to stop the reaction
  • Resorufin Reagent to standardize the assay
  • Sterile, 96-well plates
  • Non-sterile, 96-well plates to standardize the assay
  • Sterile, adhesive foil covers

The HemoFLUOR™ TE Assay Manual

How to Perform HemoFLUOR TE to Measure Time to Engraftment of Stem Cells Using a Fluorescence Readout