HemoLIGHT™ PCA

An Easy and Rapid Methylcellulose-Free Absorbance Progenitor Cell Assay for the Cell Processing Laboratory

To learn how to setup and use HemoLIGHT™ PCA, click on the video links below:

How to Perform a Progenitor Cell Assay (PCA) - An Alternative to All Methylcellulose CFU Assays

 

• Determine the number of viable cells in proliferation.
• An alternative to, or replacement for the classic colony-forming unit (CFU) assay.
• FDA, FACT and AABB alternative assays. 

• Replaces the CFU assay with an instrument-based, quantitative assay.
• No colony counting required.
• Uses an absorbance ELISA plate reader available in most laboratories.
• Incorporates the same growth factor cocktails used in MethoCult® reagents for direct assay-to-assay comparisons and correlation.
• Non-subjective, instrument-based and quantitative assay system.
• Results in 7 days depending.
• Faster and easier to use than any traditional CFU assay, even using automated colony counting.
• Simple, time efficient, flexible and more cost effective than methylcellulose CFU assays.
• Complete assay kit; includes everything needed to culture and obtain results.
• Incorporates proven Suspension Expansion Culture™ (SEC™) Technology.

(#) denotes that the Preferred Cell Systems™ formulation of this product includes TPO that stimulates the production of megakaryopoiesis. MethoCult™ and other competitor formulations of this specific product do not include TPO, do not stimulate the production of megakaryocytes and therefore do not detect the CFC-GEMM stem cell population.

*denotes that assay kits are available with 1, 2 or 4, 96-well plates

• Incorporates a terazolium WST-1 reagent that is reduced to a soluble yellow formazan, which can be measured at an maximum absorbance of 440nm (420nm to 480nm) in an absorbance plate reader.
• After culture, add 10μl of the WST-1 reagent, mix and read absorbance at 440nm after 1-4 hours in a 96-well plate reader. Plates can be returned to incubator and re-read at a later time for increased sensitivity.
• No solubilization step as in a MTT reaction. Replaces MTT assays.

• Mobilized peripheral blood
• Umbilical cord blood
• Bone marrow
• Purified cell populations for any of the above tissues

1. Standardization of the CFU-GM assay using hematopoietic growth factors. J. Hematotherapy:6:191-192 (1997)
2. Development of a novel assay to evaluate the functional potential of umbilical cord blood progenitors. Transfusion. 48:620-628 (2008).
3. Potency, Proliferation and Engraftment Potential of Stem Cell Therapeutics: The Relationship between Potency and Clinical Outcome for Hematopoietic Stem Cell Products. J Cell Sci Therapy. (2013).
4. Detecting primitive hematopoietic stem cells in total nucleated and mononuclear cell fractions from umbilical cord blood segments and units. J Translational Medicine 13:94 (2015)
5. Improving quality and potency testing for umbilical cord blood: A New Perspective. Stem Cells Translational Medicine. 4:967-973 (2015)
6. Hematopoietic stem cell potency for cellular therapeutic transplantation. In: Hematopoietic Stem Cells, Ed. Rosana P. Camacho. Published by: InTech Open Access Publisher. ISBN 978-953-307-746-8. (2011).
7. Measuring the Potency of a Stem Cell Therapeutic. In: Stem Cell Protocols. Methods in Molecular Biology, 1235, Ed. Rich IN. Published by Human Press (2015).
8. Bioluminescence Potency Measurement of Cellular Therapy Products. In: Cellular Therapy: Principles, Methods, and Regulations, 2^nd Edition, Eds. Areman EM and Loper K. Published by AABB.
9. Present Cord Blood Testing Fails to Determine if the Stem Cells Used for Transplantation are of High Quality and PotencyThe Parents Guide to Cord Blood Foundation
10. The Difference Between Stem Cell Viability and Potency: A Short Guide for Parent and Patients. The Parents Guide to Cord Blood Foundation

• HemoLIGHT™ PCA Master Mix
• WST-1 reagent
• Sterile, clear, 96-well plate(s)
• Sterile, adhesive cover foils to maintain sterility of unused wells