HemoGLO™ PMT "Global"

The Fast and Easy "Global" Bioluminescence Patient Monitoring After Transplantation (PMT) Assay for Lympho-Hematopoietic Reconstitution

 

Description Catalog Number Quantity
An Add-On Kit for Any Bioluminescence "GLO" Assay K-ATPSC-1 1 Kit

HemoGLO™ PMT

A Fast and Easy "Global" Bioluminescence Assay to Monitor Lympho-Hematopoietic Reconstitution After Transplantation

Description Tissue Formulation Catalog Number Quantity
Multiple
4-Population + Background Bone marrow or peripheral blood Low serum K6-4PMT-5 1 Kit
5-Population + Background Bone marrow or peripheral blood Low serum K6-5PMT-6 1 Kit
4-Population + Background Bone marrow or peripheral blood Low serum K6-7PMT-8 1 Kit
Multiple
4-Population + Background Bone marrow or peripheral blood Serum-Free K6SF-4PMT-5 1 Kit
5-Population + Background Bone marrow or peripheral blood Serum-Free K6SF-5PMT-6 1 Kit
7-Population + Background Bone marrow or peripheral blood Serum-Free K6SF-7PMT-8 1 Kit

 

HemoGLO™ PMT "Global": Am ATP bioluminescence assay to monitor lympho-hematopoietic reconstitution in patients after stem cell transplantation

 

  • Determine short- and long-term reconstitution of stem cells and multiple lineages, including those of the immune system (T- and B-cell lineages).
  • Short 5-7 day incubation period allows reconstitution to be monitored continually.
  • Quantitative cell population proliferation data provides all the information to predict and monitor lympho-hematopoietic reconstitution.
  • Suspension Expansion Culture™ (SEC™) Technology provides the accuracy and precision needed and makes the use of any methylcellulose CFU assay obsolete.
  • Incorporates ATP Bioluminescence Technology; the most sensitive, accurate, reliable and reproducible readout available.
  • 96-well plate format ensures smaller sample and reagent volumes with faster setup.
  • Multiplexes with flow cytometry and other readouts.
  • Directly compare reconstitution of different tissues, donors and patients over time to obtain important historical data for later use.
  • Results in less than 15 minutes.
  • Learn the assay in just a few hours.
  • Includes everything needed to culture and measure 4-, 5- or 7-populations for "global" assessment of multi-lineage hematopoietic or multi-llineage ympho-hematopoietic reconstitution.

Principle of ATP bioluminescence procedure for HemoGLO™ PMT "Global"

PLEASE NOTE: If the assay needs to be standardized and/or validated, or if proficiency testing and measurement assurance parameters are required, standards and controls are available for purchase from Preferred Cell Systems™. Alternatively, HALO® PMT "Global" can be used.

 
HemoGLO™ PMT "Global" Assay Kits are available as:
  • 4-Population "Global" Assay consists of: SC-GEMM 1, P-BFU 1, P-GM 1, P-Mk 1 and Background
  • 5-Population "Global" Assay consists of: SC-HPP 2, SC-GEMM 1, P-BFU 1, P-GM 1, P-Mk 1 and Background
  • 7-Population "Global" Assay consists of: SC-HPP 2, SC-GEMM 1, P-BFU 1, P-GM 1, P-Mk 1, P-Tcell 3, P-Bcell 2 and Background
Each HemoGLO™ PMT "Global" Assay Kit contains 5, 6 or 8 x 96-well plates, one for each lineage and background. Each sample is meant to be assessed in 8 replicates, but can be reduced to 4 replicates. Therefore, if using 8 replicates/sample, 12 samples can be assessed using a single assay kit.
  • Umbilical cord blood
  • Peripheral blood
  • Bone marrow biopsy

Tissues used for Detection

  • Peripheral blood
  • Bone marrow biopsy

The recommended cell purity is a mononuclear cell (MNC) fraction. A total nucleated cell (TNC) fraction is not recommended as this contains high concentrations of cell impurities, such as red blood cells, neutrophils, platelets and other cells that severely underestimate and even inhibit the detection of progenitor cells. 

For Research Use Only. Not for clinical diagnostic use.

Luminescence plate reader or multimode plate reader with "glow" luminescence measuring capability.
  • HALO® PMT "Global" Master Mixes
  • ATP Enumeration Reagent
  • Sterile, 96-well plates
  • Sterile, adhesive foil covers
PLEASE NOTE: If required, HemoGLO™ PMT can be standardized using the add-on ATP Bioluminescence Standardization and Calibration Kit, Catalog Number K-ATPSC-1.
 

A specific video tutorial on using the PMT assays is not yet available. Please scroll down and check out the QuickGuide and Technical Manual (if available). Below, are the links to perform the ATP bioluminescence readout:

 

QuickGuide to HemoGLO™ PMT "Global" Assays to Monitor Patients After Transplantation for Lympho-Hematopoietic Reconstitution

Download the HemoGLO™ PMT "Global" Technical Manual
Download the ATP Bioluminescence Standardization & Calibration Kit Manual
Download Certificate of Analysis (CoAs) for ATP Enumeration Reagent (ATP-ER)
Download Certificate of Analysis of ATP Reconstitution Reagent
Download Certificate of Analysis for Sterile 96-Well Plates
Download Certificate of Analysis for Sealing Films
Download Certificate of Analysis for IMDM