HemoLIGHT™ TE

The Fast and Easy Absorbance Assay to Predict Neutrophil, Platelet and/or Erythroid Engraftment After Stem Cell Transplantation

 

 

HemoLIGHT™ TE

A Fast and Easy Absorbance Assay to Predict Neutrophil, Platelet and/or Erythroid Engraftment

Description Tissue Formulation Catalog Number Quantity
Lineage-Specific Cells
P-BFU 1 Bone marrow or peripheral blood Low serum K4-BTE-1 1 Kit
P-GM1 Bone marrow or peripheral blood Low serum K4-GMTE-1 1 Kit
P-Mk 1 Bone marrow or peripheral blood Low serum K4-MkTE-1 1 Kit
P-BFU 1 + P-GM 1 + P-Mk 1 Bone marrow or peripheral blood Low serum K4-3PTE-3 1 Kit
Lineage-Specific Cells
P-BFU 1 Bone marrow or peripheral blood Serum-Free K4SF-GMTE-1 1 Kit
P-GM1 Bone marrow or peripheral blood Serum-Free K4SF-MkTE-1 1 Kit
P-Mk1 Bone marrow or peripheral blood Serum-Free K4SF-3PTE-3 1 Kit
P-BFU1 + P-GM1 + P-Mk1 Bone marrow or peripheral blood Serum-Free K4SF-BTE-1 1 Kit

An absorbance assay to predict and monitor time to engraftment after stem cell transplantation
 

Download the Time to Engraftment Assay Flyer  

 

 

A specific video tutorial on using the TE assays is not yet ready. Please scroll down and check out the QuickGuide and Technical Manual (if available). Below, is the link to perform the absorbance readout:

How to Perform an Absorbance-Based Readout (for HemoLIGHT TE)

Absorbance assays to determine time to engraftment of neutrophils, red blood cells and platelets after stem cell transplantation​

  • To rapidly predict and measure the time to neutrophil, erythroid and/or platelet engraftment after hematopoietic stem cell transplantation.
  • To promote "Best Practice Criteria Testing."
  • Measure the ability and amount of GM-CFC, BFU-E and/or Mk-CFC progenitor cell proliferation prior to detecting differentiated cells in a CFU assay.
  • Proliferation of progenitor cells can be detected within 5 days.
  • Allows for the detection of individual hematopoietic progenitor cell populations or all three simultaneously after transplantation.
  • Faster, easier and more accurate to use than any traditional CFU assay, even with automated colony counting.
  • Incorporates methylcellulose-free, Suspension Expansion Culture (SEC) Technology to produce rapid, accurate and cost-effective assays.
  • Available with low serum or serum-free formulations.

Principle of HemoLIGHT TE to Determine Time to Engraftment After Stem Cell Transplantation Using an Absorbance / Colorimetric Readout
 
The following assays are available to monitor Time to Engraftment:
  • P-BFU 1 for erythroid engraftment.
  • P-GM 1 for neutrophil engraftment
  • P-Mk 1 for platelet engraftment
  • P-BFU 1 + P-GM 1 + P Mk 1 for multilineage engraftment
Each assay can monitor a maximum of 24 samples at 4 replicates each. Higher numbers of replicates can also be used.
  • Umbilical cord blood
  • Peripheral blood
  • Bone marrow biopsy

Tissues Used for Detection

  • Peripheral blood
  • Bone marrow biopsy

The recommended cell purity is a mononuclear cell (MNC) fraction. A total nucleated cell (TNC) fraction is not recommended as this contains high concentrations of cell impurities, such as red blood cells, neutrophils, platelets and other cells that severely underestimate and even inhibit the detection of progenitor cells. 

For Research Use Only. Not for clinical diagnostic use.

Luminescence or multimode plate reader with "glow" luminescence measuring capability.

  • HemoLIGHT™ TE Master Mix(s) as either low serum or serum-free formulations
  • WST-1 reagent
  • Sterile, clear, 96-well plates
  • Sterile, adhesive foil covers

HemoLIGHT™ TE Assay Manual

QuickGuide to HemoLIGHT TE to Determine Time to Engraftment After Stem Cell Transplantation Using an Absorbance / Colorimetric Readout