HemaToxFlow™

The In Vitro Flow Cytometry Hematotoxicity Assay for 
Human and Mouse Stem and Progenitor Cells


 
  • HemaToxFlow™ Reagents are used to determine potential hematotoxicity for stem and progenitor cells using readily available human and mouse CD membrane markers and flow cytometry.
  • HemaToxFlow™ can be adapted for high-throughput flow cytometry and high-content screening.
  • HemaToxFlow™ can also be verified using HALO®-Tox HT and even the colony-forming cell (CFC) assay.
  • Using CD markers to define specific differentiated cell populations, is an alternative to the methylcellulose CFC or CFU differentiation assay. 
  • Easy, rapid and accurate dispensing using normal pipettes or liquid handlers.
  • HemaToxFlow™ allows for superior cell interactions, thereby providing: (1) shorter lag time to initiate proliferation; (2) more rapid culture completion; (3) greater sensitivity.
  • Assay kits includes 1 x 96-well plate.
  • Setup as many replicates/dose as necessary.
  • Short 5 day incubation for mouse cells and 7-9 day incubation for human cells.
  • Use any flow cytometer available.
  • Results can be verified using HALO®-Tox HT or the CFC assay, as required.

HemaToxFlow™ is available as low serum and serum-free formulations for:

  • Human
  • Mouse

HemaToxFlow™ can be used with the following fractionated cells or purified cell populations:

Human Mononuclear Cell (MNC) Fraction, CD34+ purified or CD133+ purified

  • Bone marrow
  • Umbilical cord blood
  • Peripheral blood (normal or mobilized)
Preferred Cell Systems™ recommends using Miltenyi Biotec magnetic bead technology to purify CD34 or CD133 cells.

Mouse

  • Bone marrow (cells can be fractionated to an MNC fraction and may also be purified)
  • Spleen (purified cell population may be necessary)
  • Fetal liver
  • Embryonic tissue
  • SC-GEMM 1: Primitive hematopoietic stem cell.
  • P-BFU 1: Primitive erythropoietic progenitor cell
  • P-GM 1: Primitive granulocyte-macrophage progenitor cells 
  • P-Mk 1: Primitive megakaryopoietic progenitor cell

For background controls, use HemoGro™  medium alone

The table below shows the CD antibodies used to detect different cell populations from different human and mouse HemaToxFlow™ Assay Kits. 

 

Product / Catalog Number Suggested Antibodies Cell Population Detected
HemaToxFlow™Assay Kit for Human Stem Cells*. HTF(SF)-GEMM1-100H CD34
CD133
 
Hematopoietic stem cells
Primitive hematopoietic stem cells.
(Lineage-specific cells produced from the SC-GEMM 1 stem cell population can be detected using CD antibody panels in addition to CD34 and CD133. This will be similar to differentiating and counting different colonies in a CFU assay).
HemaToxFlow™ Assay Kit for Human Erythroid Cells. HTF(SF)-BFU1-100H CD235a (GlyA) Erythroid cells
HemaToxFlow™ Assay Kit for Human Myelod Cells. HTF(SF)-GM1-100H CD13
CD14
CD15
Myelod progenitor cells
Monocytes
Granulocytes
HemaToxFlow™ Assay Kit for Human Megakaryocytes. HTF(SF)-MK1-100H CD41
CD61
Megakaryocytes
Platelets
     
HemaToxFlo™ Assay Kit for Murine Stem Cells*. HTF(SF)-GEMM1-100M CD34 Hematopoietic stem cells
HemaToxFlo™ Assay Kit for Murine Erythroid Cells. HTF(SF)-BFU1-100M CD235a
Ter-119
Erythroid cells
HemaToxFlo™ Assay Kit for Murine Myeloid Cells. HTF(SF)-GM1-100M Mac-1/CD11b
F4/80
CD66b
Gr-1/Ly6G
Ly6C
Monocytes/macrophages
Monocytes/macrophages
Granulocytes
Granulocytes
Granulocytes
HemaToxFlo™ Assay Kit for Murine Megakaryocytic. HTF(SF)-Mk1-100M CD41
CD61
Megakaryocytes/platelets
Megakaryocytes/platelets


(*) Although stem cell toxicity can be determined using the suggested antibodies, it will be noticed that to stimulate stem cells, several other growth factors and cytokines are included, many of which are lineage-specific factors. Lineage-specific cells, produced by the stem cell reagent, can also be detected using panels of the suggest antibodies for either human or mouse. Cells in specific lineages, e.g. erythroid, myeloid or megakaryocytic can then be detected using gating procedures. However, it should be noted that detecting toxicity in different lineages using the HemaToxFlo™ Stem Cell Reagent may not produce the same results obtained with the HemaToxFlow™ Reagents for individual lineages.
(SF) Add "SF" to the catalog number for serum-free reagents.

The following QuickGuide shows the steps for using HemaToxFlow™ Reagents.

 

  • HemaToxFlow™ Reagent. Please choose cell population and serum formulation.
  • Sterile, 96-well plate
  • Sterile, adhesive foil cover to maintain sterility of unused wells
Download the HemaToxFlow™ Manual
Certificate of Analysis (CoA) for HemaToxFlow™ are sent to the customer upon request 
Download the SDS Sheet

When ordering HemaToxFlow™, simply click the Catalog Number in the table below and you will be taken directly to the desired product.

LOW SERUM ASSAY KITS

Cell Population Human Mouse
P-BFU 1 HTF-BFU1-100H HTF-BFU1-100M
P-GM 1 HTF-GM1-100H HTF-GM1-100M
P-Mk 1 HTF-Mk1-100H HTF-Mk1-100M
SC-GEMM 1 HTF-GEMM1-100H HTF-GEMM1-110M

 

SERUM-FREE ASSAY KITS

Cell Population Human Mouse
P-BFU 1 HTFSF-BFU1-100H HTFSF-BFU1-100M
P-GM 1 HTFSF-GM1-100H HTFSF-GM1-100M
P-Mk 1 HTFSF-Mk1-100H HTFSF-Mk1-100M
SC-GEMM 1 HTFSF-GEMM1-100H HTFSF-GEMM1-100M