An In Vitro Absorbance Assay for Tumor Cell Viability and Function




The Easy and Rapid In Vitro Research Absorbance Assay for Tumor Cells

Cell Population Species Medium Formulation Catalog Number Quantity
Any tumor cells (adherent) Any User defined KCLL-A-1 1 Kit
Any tumor cells (non-adherent) Any User defined KCLL-NA-1 1 Kit

CellaLight™: An In Vitro Absorbance Assay for Tumor Cell Viability and Function


  • For use with primary tumor cells and transformed cell lines.
  • Determine proliferation ability and/or potential under different conditions.
  • Study the interaction of tumor cells with other normal cell types, e.g. immune cells, mesenchymal stem/progenitor cells.
  • Study the effect of agents on tumor cell growth.
  • Study tumor stem cells.
  • Incorporates the non-destructive and proven Roche® WST-1 tetrazolium reagent to produce a soluble yellow formazan product.  
  • Uses an absorbance plate reader or multimode plate reader, with a 420nm to 480nm filter.
  • Non-subjective, instrument-based and quantitative readout procedure.
  • Combine directly with other endpoint assays.
  • Flexibility to use investigators own growth medium and culture protocols.
  • Available for adherent or non-adherent cells.
  • High-throughput capability.
  • Results in 1-4 hours after addition of the single-step WST-1 reagent.
  • Simple, time efficient and cost effective.

Principle of Using STEMLight Assays on Multiple Proliferating Cell Types Using an Absorbance / Colorimetric Readout
CellaGlo™ can be used for tumor cells from multiple species. Virtually any cell line can be grown and measured in CellaGro™.
  • Incorporates a terazolium WST-1 reagent that is reduced to a soluble yellow formazan, which can be measured at an maximum absorbance of 440nm (420nm to 480nm) in an absorbance plate reader.
  • After culture, add 10μl of the WST-1 reagent, mix and read absorbance at 440nm after 1-4 hours in a 96-well plate reader. Plates can be returned to incubator and re-read at a later time for increased sensitivity.
  • No solubilization step as in a MTT reaction. Replaces MTT assays.

For Research Use Only. Not for clinical diagnostic use.
Absorbance or multimode plate reader with a 490nm filter.

  • WST-1 Reagent
  • Sterile, clear, 96-well plates. Choice of non-adherent or adherent plates.
  • Non-sterile, 96-well plate for background determination.
  • Sterile, adhesive foil covers to maintain sterility of unused wells.
Different tumor cells and cell lines have different growth requirements. For this reason, CellaLIGHT™ has the flexibility to be used with virtually any growth medium and culture protocol.