An In Vitro Bioluminescence Assay for Intestinal Viability and Function

Description Catalog Number Quantity
An Add-On Kit for Any Bioluminescence "GLO" Assay K-ATPSC-1 1 Kit


The Easy and Rapid In Vitro Research Bioluminescence Assay for Intestinal Cells

Cell Population Species Medium Formulation Catalog Number Quantity
Intestinal cells (adherent) Any User defined KEG-A-1 1 Kit
Intestinal cells (non-adherent) Any User defined KEG-NA-1 1 Kit

Epiglottis™: An In Vitro Bioluminescence Assay for Intestinal Viability and Function


  • Study intestinal epithelium stem cell research.
  • Measure stem cell self-renewal, proliferation potential and ability.
  • Study intestinal progenitor cells, cell differentiation and markers.
  • Study cell functional interaction.
  • Determine the response of crypt and villi cells to different agents.
  • The most sensitive and accurate assay readout available incorporating proven ATP bioluminescence technology.
  • Optional assay standardization using the ATP Bioluminescence Standardization & Calibration Assay Kit (Cat. No. K-ATPSC-1)
  • Measurement assurance parameters to ensure the assay is working correctly.
  • Validated according to FDA bioanalytical method validation guidelines.
  • Fast, reliable and reproducible readout with result in 15 minutes for a 96-well plate
  • High throughput capability.
  • Reliably compare results over time.
  • Multiplexing capability allowing the assay to be combined with other endpoints from the same sample.

EpoGlo™ ATP Bioluminescence Assay Principle

Performing the ATP standard curve and controls is highly recommended when using EpiGLO™. The controls are used to calibrate the instrument and the ATP standard is used to standardize the assay. The values obtained provide the measurement assurance parameters that allow you, the user, to ensure that the results obtained can be trusted.

An example of an ATP standard curve and control results are shown in the diagram below and actual values for the kit being used are given in the Technical Manual. If the values obtained are within the ranges given in the Technical Manual, the user can process the samples knowing that the results will be trustworthy.

Calibration and standardization of an ATP bioluminescence assay from Preferred Cell Systems™ - Measurement Assurance

EpiGLO™ can be used for intestinal cells from multiple species. Intestinal cell lines can also be used with EpiGLO™.

For Research Use Only. Not for clinical diagnostic use. 
Luminescence or multimode plate reader with "glow" luminescence measuring capability.

  • ATP Enumeration Reagent
  • Sterile, white, 96-well plates for hepatocyte culture.
  • Sterile, adhesive foil covers to maintain sterility of unused wells
PLEASE NOTE: If required, EpiGLO™ can be standardized using the add-on ATP Bioluminescence Standardization and Calibration Kit, Catalog Number K-ATPSC-1.

PLEASE NOTE. The investigator has the flexibility to culture intestinal epithelial cells using their own growth medium and culture protocols.
Please visit the Instructional Videos page

Download the EpiGLO™ Technical Manual
Download the ATP Bioluminescence Standardization & Calibration Kit Manual
Download Certificate of Analysis (CoAs) for ATP Enumeration Reagent (ATP-ER)
Download Certificate of Analysis for ATP Stanadrds
Download Certificate of Analysis for ATP Controls
Download Certificate of Analysis of ATP Reconstitution Reagent
Download Certificate of Analysis for Sterile 96-Well Plates
Download Certificate of Analysis for Non-Sterile, 96-Well Plates
Download Certificate of Analysis for Sealing Films
Download Certificate of Analysis for IMDM