An In Vitro Bioluminescence Assay for Tumor Cell Viability and Function



Description Catalog Number Quantity
An Add-On Kit for Any Bioluminescence "GLO" Assay K-ATPSC-1 1 Kit


The Easy and Rapid In Vitro Research Bioluminescence Assay for Tumor Cells

Cell Population Species Medium Formulation Catalog Number Quantity
Any tumor cells (adherent) Any User defined KCLG-A-1 1 Kit
Any tumor cells (non-adherent) Any User defined KCLG-NA-1 1 Kit

CellaGlo™: An In Vitro Bioluminescence Assay for Tumor Cell Viability and Function


  • For use with primary tumor cells and transformed cell lines.
  • Determine proliferation ability and/or potential under different conditions.
  • Study the interaction of tumor cells with other normal cell types, e.g. immune cells, mesenchymal stem/progenitor cells.
  • Study the effect of agents on tumor cell growth.
  • Study tumor stem cells.
  • The most sensitive and accurate assay readout available incorporating proven ATP bioluminescence technology.
  • Optional ATP bioluminescence Standardization and Calibration Kit (Cat. No. K-ATPSC-1) available (see below).
  • Measurement assurance parameters available to ensure the assay is working correctly.
  • Validated according to FDA bioanalytical method validation guidelines.
  • Fast, reliable and reproducible readout with result in 15 minutes for a 96-well plate
  • High throughput capability.
  • Reliably compare results over time.
  • Multiplexing capability allowing the assay to be combined with other endpoints from the same sample.

Principle of ATP bioluminescence technology to measure tumor cells using CellaGlo™

To properly quantify tumor cell growth, Prefrred Cell Systems™ provides an optional ATP Bioluminescence Standardization & Calibration Kit (Cat. No. K-ATPSC-1) that allows non-standardized RLU values to be converted to standardized ATP concentrations.  Performing the ATP standard curve and controls is highly recommended. The controls are used to calibrate the luminescence plate reader and the ATP standard is used to standardize the assay. The values obtained from the standard curve and controls (see diagram below) are then compared to the measurement assurance parameters that are provided in the instruction manual. If the values obtained are within the ranges provided by the measurement assurance parameters, you, the user, have successfully performed the proficiency testing to ensure that you have not only performed the assay correctly, but that the results you obtain can be trusted.

No additional proficiency testing is required if the calibration and standardization procedure is performed. The values you obtain from each calibration and standardization can be logged and used for certification that the assay has been performed correctly and that the results are trustworthy.

Calibration and standardization of an ATP bioluminescence assay from Preferred Cell Systems™ - Measurement Assurance

CellaGLO™ can be used for tumor cells from multiple species. Virtually any cell line can be grown and measured in CellaGro™.

For Research Use Only. Not for clinical diagnostic use. 
Luminescence or multimode plate reader with "glow" luminescence measuring capability.

  • ATP Enumeration Reagent
  • Sterile, white, 96-well plates for cell culture. Choice of adherent or non-adherent plates. 
  • Sterile, adhesive foil covers to maintain sterility of unused wells
PLEASE NOTE: If required, CellaGLO™ can be standardized using the add-on ATP Bioluminescence Standardization and Calibration Kit, Catalog Number K-ATPSC-1.

Different tumor cells and cell lines have different growth requirements. For this reason, CellaGLO™ has the flexibility to be used with virtually any growth medium and culture protocol. 
Please visit the Instructional Videos page

Download the CellaGLO™ Technical Manual
Download the ATP Bioluminescence Standardization & Calibration Kit Manual
Download Certificate of Analysis (CoAs) for ATP Enumeration Reagent (ATP-ER)
Download Certificate of Analysis for ATP Stanadrds
Download Certificate of Analysis for ATP Controls
Download Certificate of Analysis of ATP Reconstitution Reagent
Download Certificate of Analysis for Sterile 96-Well Plates
Download Certificate of Analysis for Non-Sterile, 96-Well Plates
Download Certificate of Analysis for Sealing Films
Download Certificate of Analysis for IMDM