An In Vitro Assay Absorbance Assay for Renal Cell Viability and Function




The Easy and Rapid In Vitro Research Absorbance Assay for Kidney Cells

Cell Population Species Medium Formulation Catalog Number Quantity
Renal Cells (adherent) Any User defined KRL-A-1 1 Kit
Renal Cells (non-adherent) Any User defined KRL-NA-1 1 Kit

RenalLight™: An In Vitro Absorbance Assay for Renal Cell Viability and Function


  • Determine the metabolic viability of different kidney cell types.
  • Study factors produced by different kidney cells types in culture, e.g. erythropoietin.
  • Determine metabolic/mitochondrial dysfunction in kidney cells.
  • Study the effect of agents on kidney function in different renal cell types.
  • Study mechanisms of renal injury.
  • Study renal disorders at the cellular and genetic levels.
  • Incorporates the non-destructive and proven Roche® WST-1 tetrazolium reagent to produce a soluble yellow formazan product.  
  • Uses an absorbance plate reader or multimode plate reader, with a 420nm to 480nm filter.
  • Non-subjective, instrument-based and quantitative readout procedure.
  • Combine directly with other endpoint assays.
  • Flexibility to use investigators own growth medium and culture protocols.
  • Available for adherent or non-adherent cells.
  • High-throughput capability.
  • Results in 1-4 hours after addition of the single-step WST-1 reagent.
  • Simple, time efficient and cost effective.

Principle of Using STEMLight Assays on Multiple Proliferating Cell Types Using an Absorbance / Colorimetric Readout
RenalGlo™ can be used for multiple species. Primary kidney cells as well as kidney cell lines, such as HepG2. Many kidney cell types have a high level of metabolic activity that can be used to determine changes in metabolic viability and other functions.
  • Incorporates a terazolium WST-1 reagent that is reduced to a soluble yellow formazan, which can be measured at an maximum absorbance of 440nm (420nm to 480nm) in an absorbance plate reader.
  • After culture, add 10μl of the WST-1 reagent, mix and read absorbance at 440nm after 1-4 hours in a 96-well plate reader. Plates can be returned to incubator and re-read at a later time for increased sensitivity.
  • No solubilization step as in a MTT reaction. Replaces MTT assays.

For Research Use Only. Not for clinical diagnostic use.
Absorbance or multimode plate reader with a 490nm filter.

  • WST-1 Reagent.
  • Sterile, clear, 96-well plates. Choice of non-adherent or adherent plates.
  • Non-sterile, 96-well plate for background determination.
  • Sterile, adhesive foil covers to maintain sterility of unused wells.
PLEASE NOTE. The investigator has the flexibility to culture renal cells using their own growth medium. RenalGro™ Growth Medium is available separately from Preferred Cell Systems™.