RenalLight™

The Easy and Rapid In Vitro Research Absorbance Assay for Kidney Cells

Cell Population	Species	Medium Formulation	Catalog Number	Quantity
Renal Cells (adherent)	Any	User defined	KRL-A-1	1 Kit
Renal Cells (non-adherent)	Any	User defined	KRL-NA-1	1 Kit

• Determine the metabolic viability of different kidney cell types.
• Study factors produced by different kidney cells types in culture, e.g. erythropoietin.
• Determine metabolic/mitochondrial dysfunction in kidney cells.
• Study the effect of agents on kidney function in different renal cell types.
• Study mechanisms of renal injury.
• Study renal disorders at the cellular and genetic levels.

• Incorporates the non-destructive and proven Roche® WST-1 tetrazolium reagent to produce a soluble yellow formazan product.  
• Uses an absorbance plate reader or multimode plate reader, with a 420nm to 480nm filter.
• Non-subjective, instrument-based and quantitative readout procedure.
• Combine directly with other endpoint assays.
• Flexibility to use investigators own growth medium and culture protocols.
• Available for adherent or non-adherent cells.
• High-throughput capability.
• Results in 1-4 hours after addition of the single-step WST-1 reagent.
• Simple, time efficient and cost effective.

 

RenalGlo™ can be used for multiple species. Primary kidney cells as well as kidney cell lines, such as HepG2. Many kidney cell types have a high level of metabolic activity that can be used to determine changes in metabolic viability and other functions.

• Incorporates a terazolium WST-1 reagent that is reduced to a soluble yellow formazan, which can be measured at an maximum absorbance of 440nm (420nm to 480nm) in an absorbance plate reader.
• After culture, add 10μl of the WST-1 reagent, mix and read absorbance at 440nm after 1-4 hours in a 96-well plate reader. Plates can be returned to incubator and re-read at a later time for increased sensitivity.
• No solubilization step as in a MTT reaction. Replaces MTT assays.

For Research Use Only. Not for clinical diagnostic use.
Absorbance or multimode plate reader with a 490nm filter.

• WST-1 Reagent.
• Sterile, clear, 96-well plates. Choice of non-adherent or adherent plates.
• Non-sterile, 96-well plate for background determination.
• Sterile, adhesive foil covers to maintain sterility of unused wells.

PLEASE NOTE. The investigator has the flexibility to culture renal cells using their own growth medium. RenalGro™ Growth Medium is available separately from Preferred Cell Systems™.