STEMLight™

An In Vitro Absorbance Assay for ES, iPS and other Transformed Cell Line Viability and Function


 

STEMLight™

The Easy and Rapid In Vitro Research Absorbance Assay for Stem Cell Lines

Cell Population Species Medium Formulation Catalog Number Quantity
ES, iPS and other stem cell lines (Adherent) Any User defined KSL-A-1 1 Kit
ES, iPS and other stem cell lines (Non-Adherent) Any User defined KSL-NA-1 1 Kit

STEMLight™: An In Vitro Absorbance Assay for ES, iPS and other Transformed Cell Line Viability and Function

 

  • Study proliferation potential and/or ability of ES, iPS or other stem cell lines.
  • Determine the effects of growth factors on stem cell maintenance and growth.
  • Study the appearance of intermittent stem cell populations of specific cell lineages.
  • Study the kinetics from stem cell proliferation to stem cell differentiation.
  • Study the effects of factors required to maintain "stemness".
  • Study the effects of CRISPR and other genetic manipulations.
  • Uses an absorbance plate reader or multiparameter plate reader, available in most laboratories.
  • Non-subjective, instrument-based and quantitative readout procedure.
  • Available for adherent or non-adherent cells.
  • Multiplexes with flow cytometric assays.
  • Multiplexes with gene expression assays etc for more information from a single sample.
  • Flexibility to use virtually any in vitro stem cell protocol, reagents and growth factors/cytokines.
  • High throughput capability.
  • Simple, time efficient and cost effective.

Principle of Using STEMLight Assays on Multiple Proliferating Cell Types Using an Absorbance / Colorimetric Readout
 
STEMGlo™ can be used for any ES, iPS or other stem cell line for virtually any species.

Cell Sources

Absorbance/colorimetric assays can be used to study virtually any cell type with proliferation capability or high metabolic activity.

  • Incorporates a terazolium WST-1 reagent that is reduced to a soluble yellow formazan, which can be measured at an maximum absorbance of 440nm (420nm to 480nm) in an absorbance plate reader.
  • After culture, add 10μl of the WST-1 reagent, mix and read absorbance at 440nm after 1-4 hours in a 96-well plate reader. Plates can be returned to incubator and re-read at a later time for increased sensitivity.
  • No solubilization step as in a MTT reaction. Replaces MTT assays.

For Research Use Only. Not for clinical diagnostic use.
Absorbance or multimode plate reader with a 490nm filter.

  • WST-1 Reagent
  • Sterile, clear, 96-well plates
  • Non-sterile, 96-well plate for background determination
  • Sterile, adhesive foil covers to maintain sterility of unused wells

STEMLight™ Assay Manual

QuickGuide to Using STEMLight Assays for Multiple Cell Types Using an Absorbance / Colorimetric Readout