STEMClone™

A Standardized and Validated Bioluminescence Methylcellulose Clonal Assay for ES, iPS and Transformed Cell Lines


 

STEMClone™

A Clonal Methylcellulose Proliferation and Differentiation Assay for Stem Cell Lines

Cell Population Species Medium Formulation Catalog Number Quantity
ES, iPS and other stem cell lines (adherent) Any STEMClone™ Methylcellulose Base Reagent KSC-A-1 1 Kit
ES, iPS and other stem cell lines (non‑adherent) Any STEMClone™ Methylcellulose Base Reagent KSC-NA-1 1 Kit


STEMClone™: A Standardized and Validated Methylcellulose Clonal Assay for ES, iPS and other Transformed Cell Lines

 

 

A complete, standardized, methylcellulose clonal proliferation and differentiation assay for any cell type that can form colonies in vitro

 

  • To determine cell clonogenicity (colony-forming ability) of ES, iPS and other stem cell lines.
  • To determine the proliferation and differentiation ability and/or potential of ES and iPS cells.
  • Allows correlation between proliferation and differentiation ability of stem cell lines.
  • Allows factors that affect stem cell clonality to be investigated.
  • Duel readouts in a single assay. Cells are allowed to form colonies which can be counted. Proliferation within the colonies can be quantified using an ATP bioluminescence readout
  • The ATP bioluminescence readout measures cell proliferation which can be standardized to allow results to be compared from different experiments.
  • Due to the correlation between colony counts (differentiation) and ATP concentration (cell proliferation), the assay allows subjective colony counts to be expressed as standardized ATP concentrations.
  • Reduces subjective colony counting high variability to a quantitative assay with low variability.
  • Incorporates proven standardized ATP bioluminescence technology used in many assays from Preferred Cell Systems™.

Principle of Standardizing a Methylcellulose Colony-Forming Assay

All ATP bioluminescence assays kits from Preferred Cell Systems™ include an ATP standard(s) and controls.  Performing the ATP standard curve and controls is highly recommended. The controls are used to calibrate the instrument and the ATP standard is used to standardize the assay. The values obtained provide the measurement assurance parameters that allow you, the user, to ensure that the results obtained can be trusted.

An example of an ATP standard curve and control results are shown in the diagram below and actual values for the kit being used are given in the Technical Manual. If the values obtained are within the ranges given in the Technical Manual, the user can process the samples knowing that the results will be trustworthy.

Calibration and standardization of an ATP bioluminescence assay from Preferred Cell Systems™ - Measurement Assurance
 
STEMClone™ can be used for ES, iPS and other stem cell lines from multiple species.

For Research Use Only. Not for clinical diagnostic use.
An inverted microscope equipped with a 10x objective and a 4x or 10x occular.
Luminescence or multimode plate reader with "glow" luminescence measuring capability.

  • STEMClone™ Methylcellulose Base Reagent.
  • ATP standard.
  • ATP controls.
  • ATP Enumeration Reagent.
  • Sterile, white, clear bottom 96-well plates. Available as non-adherent or adherent plates.
  • Non-sterile, 96-well plates.
  • Sterile, adhesive foil covers to maintain sterility of unused wells.
STEMClone™ Methylcellulose Base Reagent can be mixed with required growth medium and growth factors stimulate and differentiation ES, iPS or other stem cell lines.

STEMClone™ Assay Manual
STEMClone™ Quick Guide