B-Cell / B-Lymphocyte Progenitor Cells
Like T-cell progenitors, B-cell progenitors are not supposed to play an important role unless unless the body recognizes an infection or other perturbation that will induce the need for a higher number of B-cells to produce antibody. B-cell progenitors are produced in the bone marrow. B-cell progenitors are sometimes called Pre-B cells.
B-cell progenitors can be stimulated to a low degree with species-specific interleukins alone, but co-factors are usually needed to allow the necessary B-cell interactions to take place. As such, Preferred Cell Systems™ has colony-forming cell reagents and assays that include IL-7, but in most cases other co-stimulators are required.
More recently, Preferred Cell Systems™ has developed B-cell expansion reagents and highly sensitive and advanced, instrument-based assays to quantitatively measure B-cell progenitor stimulation for various applications. These reagents and assays can now be obtained with species-specific co-stimulators for some species and are included in the Suspension Expansion Culture™ (SEC™) Master Mix.
The assays, in particular, have been designed so that not only the proliferation status of the cells can be measured, but the type of T-cells produced can be identified using phenotypic analysis by flow cytometry. Indeed, these assays can be combined or multiplexed with several different additional readouts.
Preferred Cell Systems™ distinguishes between B-cell progenitors cultured using SEC™ Technology and those grown in methylcellulose. All T-cell progenitor using SEC™ technology are designed with the prefix "P" for "Progenitor".
In the table below, you will find the B-cell progenitor populations developed by Preferred Cell Systems™ that can be detected using either a SEC™-based or a methylcellulose assay. These are:
- CellExpand = Flexible readout of cell expansion
- HALO = Bioluminescence readout
- HemoFLUOR = Fluorescence readout
- HemoLIGHT = Absorbance/colorimetric readout
- ColonyGro & CAMEO-4 = Manual or automated colony counting
- CAMEO-96 = Manual or automated colony counting following by bioluminescence measurement
- Use or application goal (research, cellular therapy or toxicity testing).
- Whether you need to measure proliferation, differentiation or both.
- Cell type being detected.
- Instrumentation available.
- Other factors such as accuracy, sensitivity, precision etc.
| SEC™ Granulocyte/ Macrophage Progenitor |
Use / Application | SEC™ Assay / Reagent |
CFC Granulocyte/ Macrophage Cell |
CFC Assay | Growth Factor Cocktail |
|---|---|---|---|---|---|
| P-Bcell 1 | Research | CellExpand HALO HemoFLUOR HemoLIGHT |
B-CFC 1 (Pre-B) |
ColonyGro CAMEO-4 CAMEO-96 |
IL-7 only (requires co-stimulators) |
| P-Bcell 1 | In Vitro Toxicity | HALO-Tox HT (1) | B-CFC 1 (Pre-B) |
ColonyGro CAMEO-4 CAMEO-96 |
IL-7 only (requires co-stimulators) |
| P-Bcell 2 | Research | CellExpand HALO HemoFLUOR HemoLIGHT |
NA | NA | Cytokines only |
| P-Bcell 3 | Research | CellExpand HALO HemoFLUOR HemoLIGHT |
NA | NA | IgD-Dex and cytokines |
| P-Bcell 3 | Cellular therapy / Immunotherapy | HALO PMT(2) HemoFLUOR PMT(2) HemoLIGHT PMT(2) |
NA | NA | IgD-Dex and cytokines |
| P-Bcell 3 | In Vitro Toxicity | HALO-Tox HT | NA | NA | IgD-Dex and cytokines |
| All populations | Base growth medium | ImmunoGro | NA | NA |
Comments
For lymphopoietic stem cells, please see the Hematopoietic Stem Cell Page
(1) = In vitro toxicity assays for species that either do not have the required co-stimulators or have not yet been tested for those species.
(2) = Only for human cells.
Abbreviations
SEC™ = Suspension Expansion Culture™ Technology
P = Progenitor
CFC = Colony-Forming Cell
IL-7 = Interleukin-7