Immune T-Cells / T-Lymphocytes
T-cells start their life in the bone marrow from lymphopoietic stem cells. However, T-cell development occurs in the thymus. Mature T-cells are found in the circulation and in other tissues. The types of T-cells, their function and their interactions with other cells to elicit, sometimes degrading, but at other times helpful effects, particularly as an immunotherapy, are complex and difficult to analyze.
Nevertheless, to help researchers obtain the most reliable results, Preferred Cell Systems™ has developed T-cell expansion reagents and assays that need not be viewed as single products, but rather as additional tools that can also be added or modified into more complex assays to study complex functions and interactions.
For example, although mixed lymphocyte reactions (MLR) are used to better define donor and patient histocompatability, mixed lymphocyte cultures (MLC) can be used to study specific T-cells and their functions and interactions, such as agents that modulate APC-mediated T-cell activation.
T-cell activation, stimulation and/or proliferation under various situations has traditionally been measured using radioactive markers such as tritiated thymidine or radioactive chromium. All T-cell assays from Preferred Cell systems™ utilize non-radioactive readouts, and often demonstrate greater accuracy and sensitivity than radioactive markers.
In the table below, you will find the immune T-cell populations developed by Preferred Cell Systems™ that can be detected using the assays shown.
- ImmunoGLO™ TCP = Bioluminescence readout
- ImmunoFLUOR™ TCP = Fluorescence readout
- ImmunoLIGHT™ TCP = Absorbance/colorimetric readout
- Use or application goal (research, cellular therapy or toxicity testing).
- Whether you need to measure proliferation, differentiation or both.
- Cell type being detected.
- Instrumentation available.
- Other factors such as accuracy, sensitivity, precision etc.
|Use / Application||Immune Tcell
|Growth Factor Cocktail|
|Flexible to add any cytokines and/or co-stimulators|
|Tcell General||In Vitro Toxicity||ImmunoGLO-Tox HT||Flexible to add any cytokines and/or co-stimulators|
|Tcell 1||Research / Cellular, Immunetherapy||ImmunoGLO TCP 1
ImmunoFLUOR TCP 1
|IL-2 (requires co-stimulators)|
|Tcell 1||In Vitro Toxicity||ImmunoGLO-Tox HT TCP 1||IL-2 (requires co-stimulators)|
|Tcell 2||Research / Cellular, Immunetherapy||ImmunoGLO TCP 2
ImmunoFLUOR TCP 2
ImmunoLIGHT TCP 2
|CD3 and CD28|
|Tcell 2||In Vitro Toxicity||ImmunoGLO-Tox HT TCP 2||CD3 and CD28|
|Tcell 3||Research / Cellular, Immunetherapy||ImmunoGLO TCP 3
ImmunoLIGHT TCP 3
|IL-2 + CD3 + CD28|
|Tcell 3||In Vitro Toxicity||ImmunoGLO-Tox HT TCP 3||IL-2 + CD3 + CD28|
|All cell populations||Base growth medium||ImmunoGro|
TCP = T-cell proliferation
IL-2 = Interleukin 2