Complete, "Global" Bioluminescence Assay to Monitor
Hematopoietic and Immune Reconstitution After Stem Cell Transplantation


Buy HALO® PMT Assay Kits

Complete, "global" bioluminescence assay to monitor hematopoietic and immune reconstitution after stem cell transplantation.


  • To detect and quantify "global" hematopoietic reconstitution.
  • To detect and quantify "global" lympho-hematopoietic reconstitution.
  • To promote "Best Practice Criteria Testing."
  • Measurement of proliferation using highly sensitive bioluminescence readout, rather than differentiation (CFU/CFC) allows prediction of cell growth providing faster results.
  • Distinguish between balanced and unbalanced cell lineage reconstitution.
  • Allows simultaneous detection of hematopoietic stem and progenitor cells to be measured at any time to demonstrate and follow reconstitution.
  • Faster, easier and more accurate to use than any traditional CFC assay, even with automated colony counting.
  • Results obtained in only 5 days.
  • Allows comparison of patient results with time due to inclusion of assay standards and controls.
  • Incorporates proprietary Suspension Expansion Culture (SEC) growth media and ATP bioluminescence technology, to produce the most advanced and cost-effective assay system.

Principle of ATP Bioluminescence Cell Proliferation Assays
All ATP bioluminescence assays kits from Preferred Cell Systems™ include an ATP standard(s) and controls.  Performing the ATP standard curve and controls is highly recommended. The controls are used to calibrate the instrument and the ATP standard is used to standardize the assay. The values obtained provide the measurement assurance parameters that allow you, the user, to ensure that the results obtained can be trusted.

An example of an ATP standard curve and control results are shown in the diagram below and actual values for the kit being used are given in the Technical Manual. If the values obtained are within the ranges given in the Technical Manual, the user can process the samples knowing that the results will be trustworthy.

Calibration and standardization of an ATP bioluminescence assay from Preferred Cell Systems™ - Measurement Assurance

To determine "global" reconstitution:

  • 4-Population Assay for SC-GEMM 1, P-BFU 1, P-GM 1 and P-Mk 1
  • 5-Population Assay for SC-HPP 2, SC-GEMM 1, P-BFU 1, P-GM 1 and P-Mk 1
  • 7-Population Assay for SC-HPP 2, SC-GEMM 1, P-BFU 1, P-GM 1, P-Mk 1, P-Tcell, P-Bcell and background control

HALO® PMT Assays Available

Assay Type Population Measured Catalog Number
(Low serum /
Number of Plates/Kit

Number of Samples/Kit


SC-GEMM, P-BFU, P-GM, P-Mk, Control(1)

K2-4PMT-5 /
5 16(#)

P-BFU, P-GM, P-Mk, Control(2)

K2-5PMT-6 /
6 16(#)
P-BFU, P-GM, P-Mk,
P-Tcell, P-Bcell, Control(3)
K2-7PMT-8 /
8 16(#)

SC = Stem cell; P=progenitor cell

(1): To predict "global" hematopoietic reconstitution.

(2): To predict "global" hematopoietic reconstitution.

(3): To predict "global" lympho-hematopoietic reconstitution.

(#): These assay kits detect 16 samples for each cell population detected using 6 replicates/sample.

  • Umbilical cord blood
  • Peripheral blood
  • Bone marrow biopsy

Tissues used for Detection

  • Peripheral blood
  • Bone marrow biopsy

The recommended cell purity is a mononuclear cell (MNC) fraction. A total nucleated cell (TNC) fraction is not recommended as this contains high concentrations of cell impurities, such as red blood cells, neutrophils, platelets and other cells that severely underestimate and even inhibit the detection of progenitor cells. 

For Research Use Only. Not for clinical diagnostic use.

Luminescence plate reader or multimode plate reader with "glow" luminescence measuring capability.
  • HALO® PMT Master Mix
  • ATP standard
  • ATP high and low controls
  • ATP Enumeration Reagent
  • Sterile, 96-well plates
  • Non-sterile, 96-well plates
  • Sterile, adhesive foil covers

HALO® PMT Assay Manual

Complete, "global" bioluminescence assay to monitor hematopoietic and immune reconstitution after stem cell transplantation.