HALO® TE

Bioluminescence Assays to Determine Time to Engraftment of
Neutrophils, Red Blood Cells and Platelets After Stem Cell Transplantation
 

Buy HALO® TE Assay Kits
 

Bioluminescence assays to determine time to engraftment of neutrophils, red blood cells and platelets after stem cell transplantation
 

A specific video tutorial on using the TE assays is not yet ready. Please scroll down and check out the QuickGuide and Technical Manual (if available). Below, are the links to perform the ATP bioluminescence, fluorescence or absorbance readout:

How to Calibrate and Standardize an ATP Bioluminescence Assay - Part 1 (for HALO® TE)

How to Calibrate and Standardize an ATP Bioluminescence Assay - Part 2 (for HALO® TE)

Proficiency Testing for Hematopoietic Cellular Therapy Products
 

  • Rapidly predict and measure the time to neutrophil, erythroid and/or platelet engraftment after hematopoietic stem cell transplantation.
  • To promote "Best Practice Criteria Testing."
  • Measure the ability and amount of P-GM, P-BFU and/or P-Mk progenitor cell proliferation prior to detecting differentiated cells in a CFU assay.
  • Proliferation of progenitor cells can be detected within 5 days.
  • Allows for the detection of individual hematopoietic progenitor cell populations or all three simultaneously after transplantation.
  • Faster, easier and more accurate to use than any traditional CFU assay, even with automated colony counting.
  • Allows comparison of patient results over time due to inclusion of assay standards and controls.
  • HALO® TE is a validated assay.
  • Incorporates proprietary Suspension Expansion Culture (SEC) growth medium and ATP bioluminescence technology to produce the most advanced, accurate, sensitive and cost-effective assay system available.
  • Available with low serum or serum-free formulations.

Principle of ATP Bioluminescence Cell Proliferation Assays

All ATP bioluminescence assays kits from Preferred Cell Systems™ include an ATP standard(s) and controls.  Performing the ATP standard curve and controls is highly recommended. The controls are used to calibrate the instrument and the ATP standard is used to standardize the assay. The values obtained provide the measurement assurance parameters that allow you, the user, to ensure that the results obtained can be trusted.

An example of an ATP standard curve and control results are shown in the diagram below and actual values for the kit being used are given in the Technical Manual. If the values obtained are within the ranges given in the Technical Manual, the user can process the samples knowing that the results will be trustworthy.

Calibration and standardization of an ATP bioluminescence assay from Preferred Cell Systems™ - Measurement Assurance
 

When ordering HALO® TE, please use the Catalog Number. You can quickly find these products by copying the catalog number and pasting it in the advanced search.
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To determine Time to Engraftment:

  • P-BFU 1: Erythropoietic progenitor cell, used to predict red blood cell engraftment.
  • P-GM 1: Granulocyte-macrophage progenitor cell, used to determine neutrophil engraftment.
  • P-Mk 1: Megkaryopoietic progenitor cell, used to predict platelet engraftment.
HALO® TE Assays Available
Population Measured Catalog
Number
Format Number of
Plates/Kit
Number of
Samples/Kit
P-BFU 1 K2-BTE-1 Low serum 1 16-24(#)
P-BFU 1 K2SF-BTE-1 Serum-free 1 16-24(#)
P-GM 1 K2-GMTE-1 Low serum 1 16-24(#)
P-GM 1 K2SF-GMTE-1 Serum-free 1 16-24(#)
P-Mk 1 K2-MkTE-1 Low serum 1 16-24(#)
P-Mk 1 K2SF-MkTE-1 Serum-free 1 16-24(#)
P-BFU 1,
P-GM 1  P-Mk 1
K2-3PTE-1 Low serum 1 16-24(#)
P-BFU 1,
P-GM 1  P-Mk 1
K2SF-3PTE-1 Serum-free 1 16-24(#)

(#): These assay kits can detect 16-24 samples for each cell population, using 4-6 replicates/sample.

  • Umbilical cord blood
  • Peripheral blood
  • Bone marrow biopsy

Tissues Used for Detection

  • Peripheral blood
  • Bone marrow biopsy

The recommended cell purity is a mononuclear cell (MNC) fraction. A total nucleated cell (TNC) fraction is not recommended as this contains high concentrations of cell impurities, such as red blood cells, neutrophils, platelets and other cells that severely underestimate and even inhibit the detection of progenitor cells. 

For Research Use Only. Not for clinical diagnostic use.

Luminescence or multimode plate reader with "glow" luminescence measuring capability.
  • HALO® PT Master Mix (Low serum or serum-free formulations)
  • ATP standard
  • ATP high and low controls
  • ATP Enumeration Reagent
  • Sterile, 96-well plates
  • Non-sterile, 96-well plates
  • Sterile, adhesive foil covers

HALO® TE Assay Manual

Bioluminescence assays to determine time to engraftment of neutrophils, red blood cells and platelets after stem cell transplantation.