CAMEO™-4

A Minaturized Colony-Forming Unit (CFU) Assay

Cell Population	Species	Formulation	Catalog Number	Quantity
Stem Cell Populations
HPP-SP 1	Human	Low serum	KC-HPP1-50H	50mL
HPP-SP 2	Human	Low serum	KC-HPP2-50H	50mL
CFC-GEMM 1	Human	Low serum	KC-GEMM1-50H	50mL
CFC-GEMM 2	Human	Low serum	KC-GEMM2-50H	50mL
CFC-GEMM 3	Human	Low serum	KC-GEMM3-50H	50mL
CFC-GEM 1	Human	Low serum	KC-GEM1-50H	50mL
CFC-GEM 2	Human	Low serum	KC-GEM2-50H	50mL
CFC-GEM 3	Human	Low serum	KC-GEM3-50H	50mL
Progenitor Cell Populations
BFU-E 1	Human	Low serum	KC-BFU1-50H	50mL
GM-CFC 1	Human	Low serum	KC-GM1-50H	50mL
GM-CFC 2	Human	Low serum	KC-GM2-50H	50mL
GM-CFC 3	Human	Low serum	KC-GM3-50H	50mL
Mk-CFC 1	Human	Low serum	KC-Mk1-50H	50mL
Precursor Cell Populations
CFU-E	Human	Low serum	KC-E-50H	50mL
G-CFC	Human	Low serum	KC-G-50H	50mL
M-CFC	Human	Low serum	KC-M-50H	50mL
T-CFC	Human	Low serum	KC-T-50H	50mL
No Growth Factors Added
Any	Human	Low serum	KC-0-50H	50mL

CAMEO™-4

A Minaturized Colony-Forming Unit (CFU) Assay

Cell Population	Species	Formulation	Catalog Number	Quantity
Stem Cell Populations
HPP-SP 2	Mouse	Low serum	KC-HPP2-50M	50mL
CFC-GEMM 1	Mouse	Low serum	KC-GEMM1-50M	50mL
CFC-GEMM 2	Mouse	Low serum	KC-GEMM2-50M	50mL
CFC-GEM 1	Mouse	Low serum	KC-GEM1-50M	50mL
CFC-GEM 2	Mouse	Low serum	KC-GEM2-50M	50mL
Progenitor Cell Populations
BFU-E 1	Mouse	Low serum	KC-BFU1-50M	50mL
BFU-E 2	Mouse	Low serum	KC-BFU2-50M	50mL
GM-CFC 1	Mouse	Low serum	KC-GM1-50M	50mL
GM-CFC 3	Mouse	Low serum	KC-GM3-50M	50mL
Mk-CFC 1	Mouse	Low serum	KC-Mk1-50M	50mL
Precursor Cell Populations
CFU-E	Mouse	Low serum	KC-E-50M	50mL
G-CFC	Mouse	Low serum	KC-G-50M	50mL
M-CFC	Mouse	Low serum	KC-M-50M	50mL
No Growth Factors Added
Any	Mouse	Low serum	KC-0-50M	50mL

CAMEO™-4

A Minaturized Colony-Forming Unit (CFU) Assay

Cell Population	Species	Formulation	Catalog Number	Quantity
Stem Cell Populations
HPP-SP 2	Primate	Low serum	KC-HPP2-50Pr	50mL
CFC-GEMM 1	Primate	Low serum	KC-GEMM1-50Pr	50mL
Progenitor Cell Populations
BFU-E 1	Primate	Low serum	KC-BFU1-50Pr	50mL
BFU-E 2	Primate	Low serum	KC-BFU2-50Pr	50mL
GM-CFC 1	Primate	Low serum	KC-GM1-50Pr	50mL
GM-CFC 3	Primate	Low serum	KC-GM3-50Pr	50mL
Mk-CFC 1	Primate	Low serum	KC-Mk1-50Pr	50mL
Precursor Cell Populations
CFU-E	Primate	Low serum	KC-E-50Pr	50mL
G-CFC	Primate	Low serum	KC-G-50Pr	50mL
M-CFC	Primate	Low serum	KC-M-50Pr	50mL
No Growth Factors Added
Any	Primate	Low serum	KC-0-50Pr	50mL

CAMEO™-4

A Minaturized Colony-Forming Unit (CFU) Assay

Cell Population	Species	Formulation	Catalog Number	Quantity
Stem Cell Populations
HPP-SP 2	Rat	Low serum	KC-HPP2-50R	50mL
CFC-GEMM 1	Rat	Low serum	KC-GEMM1-50R	50mL
Progenitor Cell Populations
BFU-E 1	Rat	Low serum	KC-BFU1-50R	50mL
GM-CFC 1	Rat	Low serum	KC-GM1-50R	50mL
GM-CFC 3	Rat	Low serum	KC-GM3-50R	50mL
Mk-CFC 1	Rat	Low serum	KC-Mk1-50R	50mL
Precursor Cell Populations
CFU-E	Rat	Low serum	KC-E-50R	50mL
G-CFC	Rat	Low serum	KC-G-50R	50mL
M-CFC	Rat	Low serum	KC-M-50R	50mL
No Growth Factors Added
Any	Rat	Low serum	KC-0-50R	50mL

CAMEO™-4

A Minaturized Colony-Forming Unit (CFU) Assay

Cell Population	Species	Formulation	Catalog Number	Quantity
Stem Cell Populations
HPP-SP 2	Dog	Low serum	KC-HPP2-50C	50mL
CFC-GEMM 1	Dog	Low serum	KC-GEMM1-50C	50mL
Progenitor Cell Populations
BFU-E 1	Dog	Low serum	KC-BFU1-50C	50mL
GM-CFC 1	Dog	Low serum	KC-GM1-50C	50mL
GM-CFC 3	Dog	Low serum	KC-GM3-50C	50mL
Mk-CFC 1	Dog	Low serum	KC-Mk1-50C	50mL
Precursor Cell Populations
CFU-E	Dog	Low serum	KC-E-50C	50mL
G-CFC	Dog	Low serum	KC-G-50C	50mL
M-CFC	Dog	Low serum	KC-M-50C	50mL
No Growth Factors Added
Any	Dog	Low serum	KC-0-50C	50mL

CAMEO™-4

A Minaturized Colony-Forming Unit (CFU) Assay

Cell Population	Species	Formulation	Catalog Number	Quantity
Stem Cell Populations
HPP-SP 1	Horse	Low serum	KC-HPP1-50E	50mL
CFC-GEMM 1	Horse	Low serum	KC-GEMM1-50E	50mL
Progenitor Cell Populations
BFU-E 1	Horse	Low serum	KC-BFU1-50E	50mL
GM-CFC 1	Horse	Low serum	KC-GM1-50E	50mL
GM-CFC 3	Horse	Low serum	KC-GM3-50E	50mL
Mk-CFC 1	Horse	Low serum	KC-Mk1-50E	50mL
Precursor Cell Populations
CFU-E	Horse	Low serum	KC-E-50E	50mL
G-CFC	Horse	Low serum	KC-G-50E	50mL
M-CFC	Horse	Low serum	KC-M-50E	50mL
No Growth Factors Added
Any	Horse	Low serum	KC-0-50E	50mL

CAMEO™-4

A Minaturized Colony-Forming Unit (CFU) Assay

Cell Population	Species	Formulation	Catalog Number	Quantity
Stem Cell Populations
HPP-SP 2	Pig	Low serum	KC-HPP2-50Po	50mL
CFC-GEMM 1	Pig	Low serum	KC-GEMM1-50Po	50mL
Progenitor Cell Populations
BFU-E 1	Pig	Low serum	KC-BFU1-50Po	50mL
GM-CFC 1	Pig	Low serum	KC-GM1-50Po	50mL
GM-CFC 3	Pig	Low serum	KC-GM3-50Po	50mL
Mk-CFC 1	Pig	Low serum	KC-Mk1-50Po	50mL
Precursor Cell Populations
CFU-E	Pig	Low serum	KC-E-50Po	50mL
G-CFC	Pig	Low serum	KC-G-50Po	50mL
M-CFC	Pig	Low serum	KC-M-50Po	50mL
No Growth Factors Added
Any	Pig	Low serum	KC-0-50Po	50mL

CAMEO™-4

A Minaturized Colony-Forming Unit (CFU) Assay

Cell Population	Species	Formulation	Catalog Number	Quantity
Stem Cell Populations
HPP-SP 2	Sheep	Low serum	KC-HPP2-50O	50mL
CFC-GEMM 1	Sheep	Low serum	KC-GEMM1-50O	50mL
Progenitor Cell Populations
BFU-E 1	Sheep	Low serum	KC-BFU1-50O	50mL
GM-CFC 1	Sheep	Low serum	KC-GM1-50O	50mL
GM-CFC 3	Sheep	Low serum	KC-GM3-50O	50mL
Mk-CFC 1	Sheep	Low serum	KC-Mk1-50O	50mL
Precursor Cell Populations
CFU-E	Sheep	Low serum	KC-E-50O	50mL
G-CFC	Sheep	Low serum	KC-G-50O	50mL
M-CFC	Sheep	Low serum	KC-M-50O	50mL
No Growth Factors Added
Any	Sheep	Low serum	KC-0-50O	50mL

• Hematopoietic research and cellular therapy applications requiring a methylcellulose, clonal assay.
• Leukemic cell growth.
• Evaluate differentiation status or potential of different lympho-hematopoietic cell populations.
• Correlate in vivo with in vitro differentiation.
• Differentiation effects of growth factors or cytokines.
• Evaluate ex vivo differential expansion.
• Evaluate the effects of drugs, xenobiotics or other agents on the differentiation/maturation process. 

• Miniaturized assay format requires 1/10 of the volume of reagents, target cells and other sample requirements compared to traditional 35mm Petri dish CFC assays.
• Greater number of replicates (4) improves statistics.
• Smaller growth surface for more accurate and faster colony counting.
• No meniscus; allows evaluation of colonies to the well edge.
• Colonies counted after 9-10 days, compared to 14 days for MethoCult™ reagents.
• CAMEO™-4 assay kits contain sufficient reagents and culture plates for 50 samples.
• Complete flexibility; evaluate new growth factors and/or cytokines.
• Available for 17 different cell populations and 8 different species.
• Quality controlled media and superior performance compared to MethoCult™.

 

• Human
• Non-human primate (Macaca fascicularis (Cynomologus) and Macaca mulatta (Rhesus))
• Horse (Equine)
• Pig (Porcine)
• Sheep (Ovine)
• Dog (Canine)
• Rat (Rattus)
• Mouse (Murine)

Cell Type	Cell Population Detected (1)	MethoCult™ Equivalent	Growth Factor Cocktail	Number of Samples(2)	Species Available(4)
Any	None	NE	None	50	H,Pr,E,P,O,C,R,M
Stem cell	HPP-SP 1	NE	3,6,SCF,Flt3-L	50	H, M
Stem cell	HPP-SP 2	NE	EPO,GM,2,3,6,7,SCF,TPO, Flt3-L	50	H,Pr,E,P,O,C,R,M
Stem cell	CFC-GEMM 1	NE	EPO,GM,3,6,SCF,TPO, Flt3-L	50	H,Pr,E,P,O,C,R,M
Stem cell	CFC-GEMM 2	NE	EPO,GM,3,6,SCF,TPO	50	H, M
Stem cell	CFC-GEMM 3	H4435 "Enriched" MethoCult Express	EPO,GM,G,3,6,SCF TPO(3)	50	H
Stem cell	CFC-GEM 1	NE	EPO,GM3,6,SCF	50	H, M
Stem cell	CFC-GEM 2	H4034 "Optimum"	EPO,GM,G,3,SCF	50	H, M
Stem cell	CFC-GEM 3	H4434 "Classic"	EPO,GM,3,SCF	50	H
Progenitor	BFU-E 1	NE	EPO,3,SCF	50	H,Pr,E,P,O,C,R,M
Progenitor	BFU-E 2	H4430	EPO (high dose)	50	H
Precursor	CFU-E	NE	EPO (low dose)	50	H,Pr,E,P,O,C,R,M
Progenitor	GM-CFC 1	H4534 "Classic"	GM,3,SCF	50	H,Pr,E,P,O,C,R,M
Progenitor	GM-CFC 2	H4035 "Optimum"	GM,G,3,SCF	50	H
Progenitor	GM-CFC 3	NE	GM alone	50	H,Pr,E,P,O,C,R,M
Precursor	G-CFC	NE	G alone	50	H,Pr,E,P,O,C,R,M
Precursor	M-CFC	NE	M alone	50	H,Pr,E,P,O,C,R,M
Progenitor	Mk-CFC 1	NE	TPO,3,SCF	50	H,Pr,E,P,O,C,R,M
Progenitor	Mk-CFC 2	NE	TPO alone	50	H, M
Progenitor	B-CFC	NE	7 alone	50	H,Pr,E,P,C,R,M
Progenitor	T-CFC	NE	7 alone	50	H, M

NE = No equivalent product available.

(1) = Accepted nomenclature of cell populations detected by the colony-forming cell assay.

(2) = Number of samples that can be performed from 38mL by setting up a total volume of 0.6mL, including 10% cell volume), sufficient for quadruplicate 0.1mL cultures in 4-well, 35mm Petri dishes.

(3) = Contains TPO to stimulate megakaryopoiesis. The equivalent MethoCult™ product does not contain TPO and therefore cannot be designated as a CFC-GEMM stem cell population, but rather a CFC-GEM population.

(4) H = Human, Pr = Primate, E = Equine, P = Porcine, O = Ovine, C = Canine, R = Rattus, M = Murine

 

ColonyGro™ and CAMEO™-4 Incorporate the Same Growth Factor Cocktails

While ColonyGro™ uses 35mm Petri dishes, CAMEO™-4 is miniaturized using 4-wells in a 35mm Petri dish that only requires 0.1mL/well of reagent rather then 1mL of reagent. Therefore, the growth surface area for CAMEO™-4 is much smaller. As a result, the increased cell interaction allows the cells to grow faster, producing colonies in a shorter period of time. This graph shows a direct correlation between ColonyGro™ and CAMEO™-4.

Preferred Cell Systems™ provides 4 different colony-forming unit assays in 3 different formats. 

CAMEO™-4 is one of the 4 CFU assays available in a 35mm Petri dish format. Below, it is compared to the other 3 CFU assays available from Preferred Cell Systems™.

Characteristics	AllColonies™: Traditional/ Micro/Multi	ColonyGro™	CAMEO™-4	CAMEO™-96
Uses:	Basic and stem cell research. Veterinary research. Limited cellular therapy.	Basic and stem cell research. Veterinary research. Limited cellular therapy.	Basic and stem cell research. Veterinary research. Limited cellular therapy.	Basic and stem cell research. Veterinary research. Limited cellular therapy. Limited hematotoxicity testing
Assay availability:	100mL/50mL/1kit	100mL	50mL	1 kit
Number of samples:	44/50/Depends on number of replicates required(1)	44	50	Depends on number of replicates required(1)
Type of assay:	Methylcellulose  - Clonal	Methylcellulose - Clonal	Methylcellulose - Clonal	Methylcellulose - Clonal
Assay format:	35mm Petri dish/ 4-well Petri dish/ 96-well plate	35mm Petri dish	4-well Petri dish	96-well plate
Cell functionality detected:	Differentiation or cell differentiation AND proliferation	Differentiation	Differentiation	Cell differentiation AND proliferation
Readout:	Microscopic or microscopic AND plate reader	Microscopic	Microscopic	Microscopic AND plate reader
Readout endpoint:	Colony counts or colony counts WITH ATP bioluminescence	Colony counts	Colony counts	Colony counts WITH ATP bioluminescence
Subjectivity:	Subjective/subjective/ non-subjective	Subjective	Subjective	Subjective AND non-subjective
Cell types detected:	Primitive lympho- hematopoietic stem cells	Stem, progenitor and/or precursor cells	Stem, progenitor and/or precursor cells	Stem and/or progenitor cells
Species:	Human. Primate. Dog. Rat. Mouse	Human. primate. Horse. Pig. Sheep. Dog. rat. Mouse	Human. primate. Horse. Pig. Sheep. Dog. rat. Mouse	Human. primate. Horse. Pig. Sheep. Dog. rat. Mouse
MethoCult™ equivalent assays available:	No	Yes	Yes	Yes
Total culture volume required:	2.5mL/0.6mL/ Depends on replicate number (2)	2.5mL	0.6mL	Depends on replicate number (2)
Number of replicates/culture:	2/4/As many as required	2	4	As many as required
Volume/culture:	1mL/0.1mL/0.1mL	1mL	0.1mL	0.1mL
Cell culture time(3):	6-10 days	2-12 days	2-9 days	4-9 days
Instrument calibration:	Only for AllColonies™ Multi(4)	No	No	Yes(4)
Standardized assay (5):	Only for AllColonies™ Multi	No	No	Yes
Proficiency testing incorporated into assay(6):	Only for AllColonies™ Multi	No	No	Yes
Measurement assurance(7):	Only for AllColonies™ Multi	No	No	Yes
Validation(8):	Only for AllColonies™ Multi	No	No	Yes

(1) = The number of samples tested depends on the number of replicates/sample performed. For example, if 6 replicates/sample are performed, then 16 samples can be analyzed on a single 96-well plate. These samples, however, do not have to performed on the same day.
(2) = The total culture volume for AllColonies™ Multi and CAMEO™-96 depends on the number of replicates/sample performed. If 4 replicates are required, 0.6mL total volume would be prepared, while for 6 replicates, 0.8mL would be prepared.
(3) = Culture time depends on the the state of fractionation or purification of the source cells, cell population tested and species.
(4) = Instrument calibration is performed using the ATP controls included with the assay kit.
(5) = Standardization implies performing a standard curve. ATP standards are included. ColonyGro™ and CAMEO™-4 or any other methylcellulose colony-forming assay from competitors do not include standards or controls and can not be standardized. "Standardized media" does not make an assay "standardized". 
6 = Since AllColonies™ Multi and CAMEO™-96 include standards and controls that allow the results to be compared to measurement assurance parameters every time an assay is performed, proficiency testing is also performed automatically at the same time as the instrument is calibrated and the assay standardized.
7 = Measurement assurance parameters are included in the Instruction Manual.
8 = Validation parameters are included in the Instruction Manual. Since standards and controls are included with these assays, validation can be performed in-house. ColonyGro™ and CAMEO™-4 as well as all other methylcellulose colony-forming assays from competitors, do not include standards and controls and, therefore, cannot be standardized or validated.

For Research Use Only. Not for clinical diagnostic use.

Inverted microscope with a magnification of 40x - 100x.

• CAMEO™-4 reagent. Choose specific cell population and species.
• 50 x 4-well, 35mm cell culture plates.

How does CAMEO™-4 compare to ColonyGro™ or other methylcellulose reagents on the market?
CAMEO™-4 is exactly the same as ColonyGro™ with the same advantages. However, CAMEO-4 has the added advantage of being a miniaturized version of ColonyGro™ requiring less sample.
 
How many samples can be performed with a CAMEO™-4? assay kit?
50. This compared to 44 for ColonyGro™ and significantly less (about 30 samples) for all other commercially available methylcellulose reagents. In addition, CAMEO-4 assay kits include 50 culture dishes for 50 samples. No additional culture dishes are needed.
 
Are the culture dishes different?
Yes. CAMEO™-4 culture dishes are similar to 35mm Petri dishes used for ColonyGro™, but incorporate 4 small wells with very low walls. The low well walls allows the investigator to count colonies to the edge of the culture. In other words, no meniscus occurs when the methylcellulose culture is added to the wells.
 
What is the volume of each CAMEO™-4 culture?
Each of the 4 wells well contains 0.1mL of methylcellulose culture. The total volume of CAMEO-4 culture prepared is 0.6mL containing 0.06mL of cell suspension, i.e. 10% of the total culture volume.
 
What cell populations and species can be used with CAMEO™-4?
CAMEO™-4 is available for all of the same cell populations as ColonyGro™. CAMEO™-4 is also available for the same species.
 
How are colonies counted using CAMEO™-4 and are the colonies the same as ColonyGro™?
Colonies are counted in exactly the same way as ColonyGro™ using an inverted microscope. The colonies produced are similar to those for ColonyGro™  or other methylcellulose reagents.
 
Can CAMEO™-4 be use in the cell therapy processing laboratory?
Yes. CAMEO™-4 assay kits are available with exactly the same growth factor cocktail formulations as used for MethoCult™ reagents in the cell processing laboratory. There is, however, one exception. It is claimed that MethoCult™ reagent H4435 "Enriched" will stimulate CFC-GEMM. This is incorrect, because H4435 does not contain thrombopoietin (TPO) to stimulate the production of megakaryocytes. The Preferred Cell Systems™ formulation includes TPO and therefore true CFC-GEMM colonies will be produced. 
 

Download the CAMEO™-4 Manual
Download the SDS Sheet