In Vitro Assay for Immune Cells

using Bioluminescence ImmunoGlo™

 
Functional Viability and Proliferation Assays for Immune Cells Including Specialty Assays for T- and B-Lymphocytes and 1- and 2-Way Mixed Lymphocyte Cultures (MLC)
 

Buy ImmunoGlo™ Assay Kits
 

Buy ImmunoGlo™ TCP Assay Kits for T-Cells
 

Buy ImmunoGlo™ BCP Assay Kits for B-Cells
 

Buy ImmunoGlo™ MLC Assay Kits
 


 

  • ImmunoGlo™ is a general use functional immune cell proliferation assay where the investigator employs their own culture reagents and protocol for any type of immune cell.
  • ImmunoGlo™ TCP Assay Kits include specific cocktails to stimulate functional T-lymphocytes.
  • ImmunoGlo™ BCP is specific for stimulating functional human B-lymphocytes.
  • ImmunoGlo™ MLC for a 1- or 2-way mixed lymphocyte culture (MLC) or reaction (MLR).
All assays incorporate reagents to perform a standardized ATP bioluminescence readout. 
 
Uses of the ImmunoGlo™ family of assays include, but are not limited to, the following:
 
  • Lymphocyte activation and proliferation.
  • Test unprimed T-cells in the presence of antibodies, enterotoxins, mitogens etc.
  • CAR-T cell studies.
  • Cellular immune response studies.
  • Single-cell, lymphocyte cloning studies.
  • Test DLI (Donor Lymphocyte Infusion) samples for stimulation/induction prior to use.
  • Effect of accessory lymphocyte induction.
  • Effect of co-stimulators on lymphocyte induction.
  • Effect of epitope sequences and novel peptides or proteins.
  • Test the response of primed T-cells in vitro.
  • In vitro immunotoxicity studies (see Immuno™-Tox HT).
  • Immune cell gene targeting and expression, e.g. CRISPR-Cas9.
Uses of ImmunoGlo™ MLC include:
  • One-way or two-way mixed lymphocyte culture (MLC) / reaction (MLR).
  • Allogeneic mixed lymphocyte culture.
  • Dendritic cell interactions.
  • Mesenchymal stem/stromal cell interactions.
  • Agents that activate/suppress MLC/MLR.
 
The ImmunoGlo™ family of assays are the most advanced assays to measure immune cell response. The most important benefits include:  
  • The most sensitive, accurate and reliable, non-radioactive assay incorporating proven ATP bioluminescence technology.
  • Instrument-based, quantitative and fully standardized allowing comparison of results over time.
  • 10 - 100 times more sensitive than WST-1, CSFE and other absorbance or fluorescence assays.
  • High throughput capability.
  • Ability to use any mitogens, co-stimulators etc and lymphocyte culture protocols (ImmunoGlo™).
  • Ability to stimulate T-cells using IL-2 alone, CD3 and CD28 or IL-2, CD3 and CD28 cocktails ImmunoGlo™ TCP).
  • Ability to specifically study naive or activated B-lymphocyte populations (ImmunoGlo™ BCP).
  • Multiplexing capability to incorporate flow cytometry, cytokine release and other assay readouts for additional information from the same sample.

Principle of ATP Bioluminescence Used in All ImmunoGlo™ Assays

All ATP bioluminescence assays kits from Preferred Cell Systems™ include an ATP standard(s) and controls.  Performing the ATP standard curve and controls is highly recommended. The controls are used to calibrate the luminescence plate reader and the ATP standard is used to standardize the assay. The values obtained from the standard curve and controls (see diagram below) are then compared to the measurement assurance parameters that are provided in the instruction manual. If the values obtained are within the ranges provided by the measurement assurance parameters, you, the user, have successfully performed the proficiency testing to ensure that you have not only performed the assay correctly, but that the results you obtain can be trusted.

No additional proficiency testing is required if the calibration and standardization procedure is performed. The values you obtain from each calibration and standardization can be logged and used for certification that the assay has been performed correctly and that the results are trustworthy.

Calibration and standardization of an ATP bioluminescence assay from Preferred Cell Systems™ - Measurement Assurance
 
ImmunoGlo™
  • Multiple
ImmunoGlo™ TCP
  • Multiple, but not all assays available for all species. Please see online catalog.
ImmunoGlo™ BCP
  • Human
  • Mouse
  • Peripheral blood mononuclear cells (PBMC)
  • Umbilical cord blood
  • Bone marrow
  • Lymphocyte sub-populations
  • Lymphocyte tissues and organs (e.g. spleen, lymph nodes)

For Research Use Only. Not for clinical diagnostic use.

Luminescence or multimode plate reader with "glow" luminescence measuring capability.

All ImmunoGlo™, ImmunoGlo™ TCP and ImmunoGlo™ BCP contain:
  • Medium to dilute ATP standards
  • ATP standards
  • ATP controls
  • ATP Enumeration Reagent
  • Sterile, 96-well culture plates
  • Non-sterile, 96-well plates for ATP standard curve
  • Sterile, adhesive plate cover foils to maintain sterility of unused wells
ImmunoGlo™ TCP assay kits are available with the choice of:
  • IL-2 alone, or
  • CD3 and CD28 co-stimulators, or
  • IL-2 + CD3 + CD28 co-stimulators as a complete cocktail
ImmunoGlo™ BCP assay kits for human or mouse B-cells are available with the following cocktails:
  • Proprietary cocktail contains cytokines only.
  • Proprietary cocktail includes conjugates, ligands and cytokines (human)
  • Proprietary cocktail includes conjugates and cytokines (mouse).
ImmunoGlo™ MLC contains the same contents for all ImmunoGlo™ assays, but includes:
  • Mitomycin-C to perform a 1-way MLC



Complete, bioluminescence functionality and proliferation assay for immune cells.

ImmunoGlo™ TCP assays for T-lymphocytes


ImmunoGlo™ BCP assays for B-lymphocytes

Bioluminescence 1- or 2-Way Mixed Lymphocyte Culture (MLC) Assay

 

Download the ImmunoGlo™ Assay Technical Manual
Download the ATP Optimization Kit Protocol for First-Time Users
Download the ImmunoGlo™ TCP Assay Technical Manual
Download the ImmunoGlo™ BCP Assay Technical Manual
Download the ImmunoGlo™ MLC Assay Technical Manual
Download Luminometer setup and RLU to ATP conversion
Download Certificate of Analysis (CoAs) for ATP bioluminescence components. Note: Some CoAs will be included with assay kit

When ordering ImmunoGlo™click the Catalog Number in the table below and you will be taken directly to the desired product.

 

ImmunoGlo™ Assay Kits

Assay Type Catalog Number Species ImmunoGlo™
Medium containing:
Number of Plates/
Samples per Kit(#)
ImmunoGlo™ KM1-LPA-1 Multiple No cytokines or co-stimulators 1 / 16-24

 (#): Any number of replicate wells/sample can be used. Preferred Cell Systems™ recommends a minimum of 4 replicate wells/sample.
 

When ordering ImmunoGlo™ TCP click the Catalog Number in the table below and you will be taken directly to the desired product.

 

ImmunoGlo™TCP Assay Kits

Assay Type Catalog Number Species ImmunoGlo™
Medium containing:
No Plates/Samples
per Kit(#)
ImmunoGlo TCP™ KM1SF-TCP1-1H Human IL-2 alone (may require co-stimulators) 1 / 16-24
ImmunoGlo TCP™ KM1SF-TCP2-1H Human CD3 CD28 1 / 16-24
ImmunoGlo TCP™ KM1SF-TCP3-1H Human IL-2 CD3 CD28 1 / 16-24
ImmunoGlo TCP™ KM1-TCP1-1Pr Primate IL-2 alone (may require co-stimulators) 1 / 16-24
ImmunoGlo TCP™ KM1-TCP1-1R Rat IL-2 alone (may require co-stimulators) 1 / 16-24
ImmunoGlo TCP™ KM1-TCP1-1M Mouse IL-2 alone (may require co-stimulators) 1 / 16-24
ImmunoGlo TCP™ KM1-TCP1-1H Human 1 / 16-24
ImmunoGlo TCP™ KM1-TCP2-1H Human 1 / 16-24
ImmunoGlo TCP™ KM1-TCP3-1H Human 1 / 16-24

 (#): Any number of replicate wells/sample can be used. Preferred Cell Systems™ recommends a minimum of 4 replicate wells/sample.
 

When ordering ImmunoGlo™ BCP click the Catalog Number in the table below and you will be taken directly to the desired product.

 

ImmunoGlo™ BCP Assay Kits

Assay Type Catalog Number Species ImmunoGlo™
Medium containing:
No Plates/Samples
per Kit(#)
ImmunoGlo BCP™ KM1-BCP2-1H Human Cocktail contains ligands and hIL-4 1 / 16-24
ImmunoGlo BCP™ KM1-BCP3-1H Human Cocktail includes conjugates, ligands and hIL-4 1 / 16-24
ImmunoGlo BCP™ KM1-BCP3-1M Mouse Cocktail includes conjugates and mIL-4 1 / 16-24

 (#): Any number of replicate wells/sample can be used. Preferred Cell Systems™ recommends a minimum of 4 replicate wells/sample.
 

When ordering ImmunoGlo™ MLC, simply click the Catalog Number in the table below and you will be taken directly to the desired product.

 

ImmunoGlo™ MLC Assay Kits

Assay Type Catalog Number Number of Plates
ImmunoGlo MLC™ KM1-MLC-1 1