ImmunoLight™, ImmunoLight™ TCP,
ImmunoLight™ BCP, ImmunoLight™ MLC

A Family of In Vitro Absorbance Assays for 
Immune Cell Proliferation, T- and B-Cells and
Mixed Lymphocyte Culture

ImmunoLight™

An In Vitro Absorbance Basic Viability and Proliferation Assay for Immune Cells

Cell Population Species/Tissue ImmunoGro™ Medium Formulation Catalog Number Quantity
Immune cells Multiple / Any ImmunoGro™ Base Medium KM3-LPA-1 1 Kit

ImmunoLight™ TCP

An In Vitro Absorbance T-Lymphocyte Proliferation Assay

Cell Population Species/Tissue ImmunoGro™ Medium Formulation Catalog Number Quantity
T-lymphocytes Human / Any Low Serum + IL-2 KM3-TCP1-1H 1 Kit
T-lymphocytes Human / Any Low Serum + CD3 + CD28 KM3-TCP2-1H 1 Kit
T-lymphocytes Human / Any Low Serum + IL-2 + CD3 + CD28 KM3-TCP3-1H 1 Kit
T-lymphocytes Human / Any Serum-free + IL-2 KM3SF-TCP1-1H 1 Kit
T-lymphocytes Human / Any Serum-free + CD3 + CD28 KM3SF-TCP2-1H 1 Kit
T-lymphocytes Human / Any Serum-free + IL-2 + CD3 + CD28 KM3SF-TCP3-1H 1 Kit

ImmunoLight™ TCP

An In Vitro Absorbance T-Lymphocyte Proliferation Assay

Cell Population Species/Tissue ImmunoGro™ Medium Formulation Catalog Number Quantity
T-lymphocytes Primate / Any Low Serum + IL-2 KM3-TCP1-1Pr 1 Kit

ImmunoLight™ TCP

An In Vitro Absorbance T-Lymphocyte Proliferation Assay

Cell Population Species/Tissue ImmunoGro™ Medium Formulation Catalog Number Quantity
T-lymphocytes Rat / Any Low Serum + IL-2 KM3-TCP1-1R 1 Kit

ImmunoLight™ TCP

An In Vitro Absorbance T-Lymphocyte Proliferation Assay

Cell Population Species/Tissue ImmunoGro™ Medium Formulation Catalog Number Quantity
T-lymphocytes Mouse / Any Low Serum + IL-2 KM3-TCP1-1M 1 Kit

ImmunoLight™ BCP

An In Vitro Absorbance B-Lymphocyte Proliferation Assay

Cell Population Species/Tissue ImmunoGro™ Medium Formulation Catalog Number Quantity
B-lymphocytes Human / Any Low Serum +Ligands+IL4 KM3-BCP2-1H 1 Kit
B-lymphocytes Human / Any Low Serum+Conjugates+Ligands+IL-4 KM3-BCP3-1H 1 Kit

ImmunoLight™ BCP

An In Vitro Absorbance B-Lymphocyte Proliferation Assay

Cell Population Species/Tissue ImmunoGro™ Medium Formulation Catalog Number Quantity
B-lymphocytes Mouse / Any Low Serum+Conjugates+IL-4 KM3-BCP3-1M 1 Kit

ImmunoLight™ MLC

An In Vitro Absorbance 1- or 2-Way Mixed Lymphocyte Culture (MLC) Assay

Cell Population Species/Tissue ImmunoGro™ Medium Formulation Catalog Number Quantity
Immune cells Multiple / Any Includes Mitomycin-C for 1-way MLC KM3-MLC-1 1 Kit

 

ImmunoLight Assays: A family of in vitro absorbance assays for immune proliferation of t- and B-cells and mixed lymphocyte culture/reactions

 

 


 

  • ImmunoLight™ is a general use functional immune cell proliferation assay where the investigator employs their own culture reagents and protocol for any type of immune cell.
  • ImmunoLight™ TCP Assay Kits include specific cocktails to stimulate functional T-lymphocytes.
  • ImmunoLight™ BCP is specific for stimulating functional human B-lymphocytes.
  • ImmunoLight™ MLC for 1- or 2-Way Mixed Lymphocyte Culture (MLC).
Uses of the ImmunoLight™ family of assays include, but are not limited to, the following:  
  • Lymphocyte activation.
  • T-lymphocyte proliferation (see also ImmunoLight™-96 TCP)
  • Test unprimed T-cells in the presence of antibodies, enterotoxins, mitogens etc.
  • Cellular immune response studies.
  • Single cell, T-lymphocyte cloning studies.
  • Test DLI (Donor Lymphocyte Infusion) samples for stimulation/induction prior to use.
  • Effect of accessory (non T-cells) on T cell induction.
  • Effect of co-stimulators on T-cell induction.
  • Effect of epitope sequences and novel peptides or proteins.
  • Test the response of primed T-cells in vitro.
Uses of ImmunoLight™ MLC include:
  • One-way or two-way mixed lymphocyte culture (MLC) / reaction (MLR).
  • Allogeneic mixed lymphocyte culture.
  • Dendritic cell interactions.
  • Mesenchymal stem/stromal cell interactions.
  • Agents that activate/suppress MLC/MLR.
 
  • Uses a colorimetric/absorbance ELISA plate reader, available in most laboratories.
  • Non-subjective, instrument-based and quantitative.
  • Flexibility to use virtually any protocol, reagents and growth factors/cytokines or other immune agent stimulators or suppressors.
  • Assay kit includes supplies for culture and cell processing.
  • Simple, time efficient and cost effective.

Principle of the ImmunoLight Assays for Immune Responses Using an Absorbance / Colorimetric Readout
 
ImmunoLight™
  • Multiple
ImmunioLight™ TCP
  • Multiple, but not all assays available for all species. Please see online catalog.
ImmunoLight™ BCP
  • Human
For all assays:
  • Peripheral blood mononuclear cells (PBMC)
  • Umbilical cord blood
  • Bone marrow
  • Lymphocyte sub-populations
  • Lymphocyte tissues and organs (e.g. spleen, lymph nodes)

For Research Use Only. Not for clinical diagnostic use.

Absorbance plate reader or multimode plate reader with an absorbance filter at 490nm.

All ImmunoLight™ assay kits contain:
  • WST-1 Reagent
  • Sterile, clear 96-well plates
  • Sterile, adhesive foil covers
ImmunoLight™ TCP assay kits are available with the choice of:
  • IL-2 alone, or
  • CD3 + CD28 co-stimulators, or
  • IL-2 + CD3 + CD28 co-stimulators as a complete cocktail
ImmunoGlo™ BCP assay kits for human or mouse B-cells are available with the following cocktails:
  • Proprietary cocktail contains cytokines only.
  • Proprietary cocktail includes conjugates, ligands and cytokines (human)
  • Proprietary cocktail includes conjugates and cytokines (mouse).
ImmunoLight™ MLC contains the same contents for all ImmunoLight™ assays, but includes:
  • Mitomycin-C to perform a 1-way MLC

QuickGuide for ImmunoLight to Measure Immune Responses Using an Absorbance / Colorimetric Readout

QuickGuide to Perform ImmunoLight MLC (Mixed Lymphocyte Culture) Using an Absorbance / Colorimetric Readout
 

 

 

Download the ImmunoLight™ MLC Technical Manual