ImmunoFluor™, ImmunoFluor™ TCP,
ImmunoFluor™ BCP, ImmunoFluor™ MLC

A Family of In Vitro Fluorescence Assays for
Immune Cell Proliferation, T- and B-Cells and
Mixed Lymphocyte Culture

ImmunoFluor™

An In Vitro Fluorescence Basic Viability and Proliferation Assay for Immune Cells

Cell Population Species/Tissue ImmunoGro™ Medium Formulation Catalog Number Quantity
Immune cells Multiple / Any ImmunoGro™ Base Medium KM2-LPA-1 1 Kit

ImmunoFluor™ TCP

An In Vitro Fluorescence T-Lymphocyte Proliferation Assay

Cell Population Species/Tissue ImmunoGro™ Medium Formulation Catalog Number Quantity
T-lymphocytes Human / Any Low Serum + IL-2 KM2-TCP1-1H 1 Kit
T-lymphocytes Human / Any Low Serum + CD3 + CD28 KM2-TCP2-1H 1 Kit
T-lymphocytes Human / Any Low Serum + IL-2 + CD3 + CD28 KM2-TCP3-1H 1 Kit
T-lymphocytes Human / Any Serum-free + IL-2 KM2SF-TCP1-1H 1 Kit
T-lymphocytes Human / Any Serum-free + CD3 + CD28 KM2SF-TCP2-1H 1 Kit
T-lymphocytes Human / Any Serum-free + IL-2 + CD3 + CD28 KM2SF-TCP3-1H 1 Kit

ImmunoFluor™ TCP

An In Vitro Fluorescence T-Lymphocyte Proliferation Assay

Cell Population Species/Tissue ImmunoGro™ Medium Formulation Catalog Number Quantity
T-lymphocytes Primate / Any Low Serum + IL-2 KM2-TCP1-1Pr 1 Kit

ImmunoFluor™ TCP

An In Vitro Fluorescence T-Lymphocyte Proliferation Assay

Cell Population Species/Tissue ImmunoGro™ Medium Formulation Catalog Number Quantity
T-lymphocytes Rat / Any Low Serum + IL-2 KM2-TCP1-1R 1 Kit

ImmunoFluor™ TCP

An In Vitro Fluorescence T-Lymphocyte Proliferation Assay

Cell Population Species/Tissue ImmunoGro™ Medium Formulation Catalog Number Quantity
T-lymphocytes Mouse / Any Low Serum + IL-2 KM2-TCP1-1M 1 Kit

ImmunoFluor™ BCP

An In Vitro Fluorescence B-Lymphocyte Proliferation Assay

Cell Population Species/Tissue ImmunoGro™ Medium Formulation Catalog Number Quantity
B-lymphocytes Human / Any Low Serum +Ligands+IL4 KM2-BCP2-1H
B-lymphocytes Human / Any Low Serum+Conjugates+Ligands+IL-4 KM2-BCP3-1H

ImmunoFluor™ BCP

An In Vitro Fluorescence B-Lymphocyte Proliferation Assay

Cell Population Species/Tissue ImmunoGro™ Medium Formulation Catalog Number Quantity
B-lymphocytes Mouse / Any Low Serum+Conjugates+IL-4 KM2-BCP3-1M 1 Kit

ImmunoFluor™ MLC

An In Vitro Fluorescence 1- or 2-Way Mixed Lymphocyte Culture (MLC) Assay

Cell Population Species/Tissue ImmunoGro™ Medium Formulation Catalog Number Quantity
Immune Cells Multiple / Any Includes Mitomycin-C for 1-way MLC KM2-MLC-1 1 Kit

 

ImmunoFLUOR Assays: A family of in vitro fluorescence assays for immune cell proliferation, T- and B-cell proliferation and mixed lymphocyte culture/reactions

 

 

 


 

  • ImmunoFluor™ is a general use functional immune cell proliferation assay where the investigator employs their own culture reagents and protocol for any type of immune cell.
  • ImmunoFluor™ TCP Assay Kits include specific cocktails to stimulate functional T-lymphocytes.
  • ImmunoFluor™ BCP is specific for stimulating functional human B-lymphocytes.
  • ImmunoFluor™ MLC for a 1- or 2-way mixed lymphocyte culture (MLC) or reaction (MLR).
All assays incorporate reagents for a fluorescence readout. 
 
  • Lymphocyte activation.
  • T-lymphocyte proliferation.
  • Test unprimed lymphocytes in the presence of antibodies, enterotoxins, mitogens etc.
  • Cellular immune response studies.
  • Single-cell, lymphocyte cloning studies.
  • Test DLI (Donor Lymphocyte Infusion) samples for stimulation/induction prior to use.
  • Effect of accessory on lymphocytes induction.
  • Effect of co-stimulators on lymphocytes induction.
  • Effect of epitope sequences and novel peptides or proteins.
  • Test the response of primed T-cells in vitro.
Uses of ImmunoFluor™ MLC include:
  • One-way or two-way mixed lymphocyte culture (MLC) / reaction (MLR).
  • Allogeneic mixed lymphocyte culture.
  • Dendritic cell interactions.
  • Mesenchymal stem/stromal cell interactions.
  • Agents that activate/suppress MLC/MLR.
  • Non-subjective, instrument-based and quantitative.
  • Easily multiplexes with flow cytometry protocols using other fluorescent labels, e.g. FITC, PE, PECy5, PerCP-Cy5, PE-Cy7 etc.
  • Multiplexes with bioluminescence assays.
  • Use in place of colorimetric/absorbance assays for greater sensitivity.
  • Assay kit includes supplies for culture and cell processing.
  • Simple, time efficient and cost effective. 

Principle of detecting an immune cell, T-cell or B-cell response using ImmunoFluor fluorescence assay kits
 
ImmunoFluor™ and ImmunoFluor™ TCP
  • Human
  • Non-human primate
  • Mouse
ImmunoFluor™ BCP
  • Human
  • Mouse
  • Peripheral blood mononuclear cells (PBMC)
  • Lymphocyte sub-populations
  • Lymphocyte tissues and organs

For Research Use Only. Not for clinical diagnostic use.

Fluorescence or multimode plate reader with an excitation filter of 560nm and emission filter of 590nm.

All ImmunoFluor™ Assay Kits contain:
  • ​Resazurin Reagent
  • 3% SDS as stop reagent
  • Resorufin to standardize the assay
  • Non-sterile, 96-well plates for standardizing the assay
  • Sterile, 96-well plates
  • Sterile, adhesive foil covers
ImmunoFluor™ TCP assay kits are available with the choice of:
  • IL-2 alone, or
  • CD3 + CD28 co-stimulators, or
  • IL-2 + CD3 + CD28 co-stimulators as a complete cocktail
ImmunoGlo™ BCP assay kits for human or mouse B-cells are available with the following cocktails:
  • Proprietary cocktail contains cytokines only.
  • Proprietary cocktail includes conjugates, ligands and cytokines (human)
  • Proprietary cocktail includes conjugates and cytokines (mouse).
ImmunoFluor™ MLC contains the same contents for all ImmunoFluor™ assays, but includes:
  • Mitomycin-C to perform a 1-way MLC

How to measure an immune cell response using the ImmunoFluor™ fluorescence assay kit


How to measure a mixed lymphocyte culture response using the ImmunoFluor MLC fluorescence assay kit