ImmunoFluor™, ImmunoFluor™ TCP,
ImmunoFluor™ BCP, ImmunoFluor™ MLC
A Family of In Vitro Fluorescence Assays for
Immune Cell Proliferation, T- and B-Cells and
Mixed Lymphocyte Culture
Buy ImmunoFluor™
ImmunoFluor™
An In Vitro Fluorescence Basic Viability and Proliferation Assay for Immune Cells
Cell Population | Species/Tissue | ImmunoGro™ Medium Formulation | Catalog Number | Quantity |
---|---|---|---|---|
Immune cells | Multiple / Any | ImmunoGro™ Base Medium | KM2-LPA-1 | 1 Kit |
ImmunoFluor™ TCP
An In Vitro Fluorescence T-Lymphocyte Proliferation Assay
Cell Population | Species/Tissue | ImmunoGro™ Medium Formulation | Catalog Number | Quantity |
---|---|---|---|---|
T-lymphocytes | Human / Any | Low Serum + IL-2 | KM2-TCP1-1H | 1 Kit |
T-lymphocytes | Human / Any | Low Serum + CD3 + CD28 | KM2-TCP2-1H | 1 Kit |
T-lymphocytes | Human / Any | Low Serum + IL-2 + CD3 + CD28 | KM2-TCP3-1H | 1 Kit |
T-lymphocytes | Human / Any | Serum-free + IL-2 | KM2SF-TCP1-1H | 1 Kit |
T-lymphocytes | Human / Any | Serum-free + CD3 + CD28 | KM2SF-TCP2-1H | 1 Kit |
T-lymphocytes | Human / Any | Serum-free + IL-2 + CD3 + CD28 | KM2SF-TCP3-1H | 1 Kit |
ImmunoFluor™ TCP
An In Vitro Fluorescence T-Lymphocyte Proliferation Assay
Cell Population | Species/Tissue | ImmunoGro™ Medium Formulation | Catalog Number | Quantity |
---|---|---|---|---|
T-lymphocytes | Primate / Any | Low Serum + IL-2 | KM2-TCP1-1Pr | 1 Kit |
ImmunoFluor™ TCP
An In Vitro Fluorescence T-Lymphocyte Proliferation Assay
Cell Population | Species/Tissue | ImmunoGro™ Medium Formulation | Catalog Number | Quantity |
---|---|---|---|---|
T-lymphocytes | Rat / Any | Low Serum + IL-2 | KM2-TCP1-1R | 1 Kit |
ImmunoFluor™ TCP
An In Vitro Fluorescence T-Lymphocyte Proliferation Assay
Cell Population | Species/Tissue | ImmunoGro™ Medium Formulation | Catalog Number | Quantity |
---|---|---|---|---|
T-lymphocytes | Mouse / Any | Low Serum + IL-2 | KM2-TCP1-1M | 1 Kit |
ImmunoFluor™ BCP
An In Vitro Fluorescence B-Lymphocyte Proliferation Assay
Cell Population | Species/Tissue | ImmunoGro™ Medium Formulation | Catalog Number | Quantity |
---|---|---|---|---|
B-lymphocytes | Human / Any | Low Serum +Ligands+IL4 | KM2-BCP2-1H | |
B-lymphocytes | Human / Any | Low Serum+Conjugates+Ligands+IL-4 | KM2-BCP3-1H | |
ImmunoFluor™ BCP
An In Vitro Fluorescence B-Lymphocyte Proliferation Assay
Cell Population | Species/Tissue | ImmunoGro™ Medium Formulation | Catalog Number | Quantity |
---|---|---|---|---|
B-lymphocytes | Mouse / Any | Low Serum+Conjugates+IL-4 | KM2-BCP3-1M | 1 Kit |
ImmunoFluor™ MLC
An In Vitro Fluorescence 1- or 2-Way Mixed Lymphocyte Culture (MLC) Assay
Cell Population | Species/Tissue | ImmunoGro™ Medium Formulation | Catalog Number | Quantity |
---|---|---|---|---|
Immune Cells | Multiple / Any | Includes Mitomycin-C for 1-way MLC | KM2-MLC-1 | 1 Kit |

The ImmunoFluor™ Assay Family
- ImmunoFluor™ is a general use functional immune cell proliferation assay where the investigator employs their own culture reagents and protocol for any type of immune cell.
- ImmunoFluor™ TCP Assay Kits include specific cocktails to stimulate functional T-lymphocytes.
- ImmunoFluor™ BCP is specific for stimulating functional human B-lymphocytes.
- ImmunoFluor™ MLC for a 1- or 2-way mixed lymphocyte culture (MLC) or reaction (MLR).
- Lymphocyte activation.
- T-lymphocyte proliferation.
- Test unprimed lymphocytes in the presence of antibodies, enterotoxins, mitogens etc.
- Cellular immune response studies.
- Single-cell, lymphocyte cloning studies.
- Test DLI (Donor Lymphocyte Infusion) samples for stimulation/induction prior to use.
- Effect of accessory on lymphocytes induction.
- Effect of co-stimulators on lymphocytes induction.
- Effect of epitope sequences and novel peptides or proteins.
- Test the response of primed T-cells in vitro.
- One-way or two-way mixed lymphocyte culture (MLC) / reaction (MLR).
- Allogeneic mixed lymphocyte culture.
- Dendritic cell interactions.
- Mesenchymal stem/stromal cell interactions.
- Agents that activate/suppress MLC/MLR.
- Non-subjective, instrument-based and quantitative.
- Easily multiplexes with flow cytometry protocols using other fluorescent labels, e.g. FITC, PE, PECy5, PerCP-Cy5, PE-Cy7 etc.
- Multiplexes with bioluminescence assays.
- Use in place of colorimetric/absorbance assays for greater sensitivity.
- Assay kit includes supplies for culture and cell processing.
- Simple, time efficient and cost effective.
ImmunoFluor™ and ImmunoFluor™ TCP
Tissues
- Human
- Non-human primate
- Mouse
- Human
- Mouse
- Peripheral blood mononuclear cells (PBMC)
- Lymphocyte sub-populations
- Lymphocyte tissues and organs
For Research Use Only. Not for clinical diagnostic use.
Fluorescence or multimode plate reader with an excitation filter of 560nm and emission filter of 590nm.
All ImmunoFluor™ Assay Kits contain:
QuickGuides
- €‹Resazurin Reagent
- 3% SDS as stop reagent
- Resorufin to standardize the assay
- Non-sterile, 96-well plates for standardizing the assay
- Sterile, 96-well plates
- Sterile, adhesive foil covers
- IL-2 alone, or
- CD3 + CD28 co-stimulators, or
- IL-2 + CD3 + CD28 co-stimulators as a complete cocktail
- Proprietary cocktail contains cytokines only.
- Proprietary cocktail includes conjugates, ligands and cytokines (human)
- Proprietary cocktail includes conjugates and cytokines (mouse).
- Mitomycin-C to perform a 1-way MLC