In Vitro Assay for Immune Cells

using Fluorescence ImmunoFluor™

 
Functional Viability and Proliferation Assays for Immune Cells Including Specialty Assays for T- and B-Lymphocytes and 1- and 2-Way Mixed Lymphocyte Cultures (MLC)
 

Buy ImmunoFluor™ Assay Kits
 

Buy ImmunoFluor™ TCP Assay Kits for T-Cells
 

Buy ImmunoFluor™ BCP Assay Kits for B-Cells
 

Buy ImmunoFluor™ MLC Assay Kits
 


 

  • ImmunoFluor™ is a general use functional immune cell proliferation assay where the investigator employs their own culture reagents and protocol for any type of immune cell.
  • ImmunoFluor™ TCP Assay Kits include specific cocktails to stimulate functional T-lymphocytes.
  • ImmunoFluor™ BCP is specific for stimulating functional human B-lymphocytes.
  • ImmunoFluor™ MLC for a 1- or 2-way mixed lymphocyte culture (MLC) or reaction (MLR).
All assays incorporate reagents for a fluorescence readout. 
 
  • Lymphocyte activation.
  • T-lymphocyte proliferation.
  • Test unprimed lymphocytes in the presence of antibodies, enterotoxins, mitogens etc.
  • Cellular immune response studies.
  • Single-cell, lymphocyte cloning studies.
  • Test DLI (Donor Lymphocyte Infusion) samples for stimulation/induction prior to use.
  • Effect of accessory on lymphocytes induction.
  • Effect of co-stimulators on lymphocytes induction.
  • Effect of epitope sequences and novel peptides or proteins.
  • Test the response of primed T-cells in vitro.
Uses of ImmunoFluor™ MLC include:
  • One-way or two-way mixed lymphocyte culture (MLC) / reaction (MLR).
  • Allogeneic mixed lymphocyte culture.
  • Dendritic cell interactions.
  • Mesenchymal stem/stromal cell interactions.
  • Agents that activate/suppress MLC/MLR.
  • Non-subjective, instrument-based and quantitative.
  • Easily multiplexes with flow cytometry protocols using other fluorescent labels, e.g. FITC, PE, PECy5, PerCP-Cy5, PE-Cy7 etc.
  • Multiplexes with bioluminescence assays.
  • Use in place of colorimetric/absorbance assays for greater sensitivity.
  • Assay kit includes supplies for culture and cell processing.
  • Simple, time efficient and cost effective. 

Principle of detecting an immune cell, T-cell or B-cell response using ImmunoFluor fluorescence assay kits
 
ImmunoFluor™ and ImmunoFluor™ TCP
  • Human
  • Non-human primate
  • Mouse
ImmunoFluor™ BCP
  • Human
  • Mouse
  • Peripheral blood mononuclear cells (PBMC)
  • Lymphocyte sub-populations
  • Lymphocyte tissues and organs

For Research Use Only. Not for clinical diagnostic use.

Fluorescence or multimode plate reader with an excitation filter of 560nm and emission filter of 590nm.

All ImmunoFluor™ Assay Kits contain:
  • ​Resazurin Reagent
  • 3% SDS as stop reagent
  • Resorufin to standardize the assay
  • Non-sterile, 96-well plates for standardizing the assay
  • Sterile, 96-well plates
  • Sterile, adhesive foil covers
ImmunoFluor™ TCP assay kits are available with the choice of:
  • IL-2 alone, or
  • CD3 + CD28 co-stimulators, or
  • IL-2 + CD3 + CD28 co-stimulators as a complete cocktail
ImmunoGlo™ BCP assay kits for human or mouse B-cells are available with the following cocktails:
  • Proprietary cocktail contains cytokines only.
  • Proprietary cocktail includes conjugates, ligands and cytokines (human)
  • Proprietary cocktail includes conjugates and cytokines (mouse).
ImmunoFluor™ MLC contains the same contents for all ImmunoFluor™ assays, but includes:
  • Mitomycin-C to perform a 1-way MLC

How to measure an immune cell response using the ImmunoFluor™ fluorescence assay kit


How to measure a mixed lymphocyte culture response using the ImmunoFluor MLC fluorescence assay kit
 

 

 

When ordering ImmunoFluor™, ImmunoFluor™ TCP or ImmunoFluor™ BCP, simply click on the Catalog Number in the table below and you will be taken directly to the desired product.

ImmunoFluor™ Assays

Assay Type Catalog Number Species ImmunoGro™ Medium containing: Number of Plates/
Samples per Kit(#)
ImmunoFLUOR™ KM2-LPA-1 Multiple User defined 1 / 16-24
(#): Any number of replicate wells/ sample can be performed. However, Preferred Cell Systems™ recommends a minimum of 4 replicate wells/sample.  

 

When ordering ImmunoFluor™ TCP, simply click on the Catalog Number in the table below and you will be taken directly to the desired product.

ImmunoFluor™ TCP Assays

Assay Type Catalog Number Species ImmunoGro™ Medium containing: No. Plates/Samples per Kit(#)
ImmunoFluor TCP™ KM2-TCP1-1H Human IL-2 alone (may require co-stimulators) 1 / 16-24
ImmunoFluor TCP™ KM2-TCP1-1M Mouse IL-2 alone (may require co-stimulators) 1 / 16-24
ImmunoFluor TCP™ KM2-TCP1-1Pr Primate IL-2 alone (may require co-stimulators) 1 / 16-24
ImmunoFluor TCP™ KM2-TCP1-1R Rat IL-2 alone (may require co-stimulators) 1 / 16-24
ImmunoFluor TCP™ KM2-TCP2-1H Human CD3 and CD28 1 / 16-24
ImmunoFluor TCP™ KM2-TCP3-1H Human IL-2 and CD3 and CD28 1 / 16-24
ImmunoFluor TCP™ KM2SF-TCP1-1H Human IL-2 alone (may require co-stimulators) 1 / 16-24
ImmunoFluor TCP™ KM2SF-TCP2-1H Human CD3 and CD28 1 / 16-24
ImmunoFluor TCP™ KM2SF-TCP3-1H Human IL-2 and CD3 and CD28 1 / 16-24
(#): Any number of replicate wells/ sample can be performed. However, Preferred Cell Systems™ recommends a minimum of 4 replicate wells/sample.  

 

When ordering ImmunoFluor™ BCP, simply click on the Catalog Number in the table below and you will be taken directly to the desired product.

ImmunoFluor™ BCP Assay Kits

Assay Type Catalog Number Species ImmunoGro™ Medium containing: No. Plates/Samples per Kit(#)
ImmunoFluor BCP™ KM2-BCP2-1H Human Cocktail contains ligands and hIL-4 1 / 16-24
ImmunoFluor BCP™ KM2-BCP3-1H Human Cocktail includes conjugates, ligands and hIL-4 1 / 16-24
ImmunoFluor BCP™ KM2-BCP3-1M Mouse Cocktail includes conjugates and mIL-4 1 / 16-24
(#): Any number of replicate wells/ sample can be performed. However, Preferred Cell Systems™ recommends a minimum of 4 replicate wells/sample.  

 

When ordering ImmunoFluor™ MLC, simply click the Catalog Number in the table below and you will be taken directly to the desired product.

 

ImmunoFluor™ MLC Assay Kits

Assay Type Catalog Number Number of Plates
ImmunoFluor MLC™ MLC KM2-MLC-1 1