In Vitro Assay for Immune Cells

using Absorbance ImmunoLight™

 
Functional Viability and Proliferation Assays for Immune Cells Including Specialty Assays for T- and B-Lymphocytes and 1- and 2-Way Mixed Lymphocyte Cultures (MLC)
 

Buy ImmunoLight™ Assay Kits

 

Buy ImmunoLight™ TCP Assay Kits for T-Cells

 

Buy ImmunoLight™ BCP Assay Kits for B-Cells

 

Buy ImmunoLight™ MLC Assay Kits

 


 

  • ImmunoLight™ is a general use functional immune cell proliferation assay where the investigator employs their own culture reagents and protocol for any type of immune cell.
  • ImmunoLight™ TCP Assay Kits include specific cocktails to stimulate functional T-lymphocytes.
  • ImmunoLight™ BCP is specific for stimulating functional human B-lymphocytes.
  • ImmunoLight™ MLC for 1- or 2-Way Mixed Lymphocyte Culture (MLC).
Uses of the ImmunoLight™ family of assays include, but are not limited to, the following:  
  • Lymphocyte activation.
  • T-lymphocyte proliferation (see also ImmunoLight™-96 TCP)
  • Test unprimed T-cells in the presence of antibodies, enterotoxins, mitogens etc.
  • Cellular immune response studies.
  • Single cell, T-lymphocyte cloning studies.
  • Test DLI (Donor Lymphocyte Infusion) samples for stimulation/induction prior to use.
  • Effect of accessory (non T-cells) on T cell induction.
  • Effect of co-stimulators on T-cell induction.
  • Effect of epitope sequences and novel peptides or proteins.
  • Test the response of primed T-cells in vitro.
Uses of ImmunoLight™ MLC include:
  • One-way or two-way mixed lymphocyte culture (MLC) / reaction (MLR).
  • Allogeneic mixed lymphocyte culture.
  • Dendritic cell interactions.
  • Mesenchymal stem/stromal cell interactions.
  • Agents that activate/suppress MLC/MLR.
 
  • Uses a colorimetric/absorbance ELISA plate reader, available in most laboratories.
  • Non-subjective, instrument-based and quantitative.
  • Flexibility to use virtually any protocol, reagents and growth factors/cytokines or other immune agent stimulators or suppressors.
  • Assay kit includes supplies for culture and cell processing.
  • Simple, time efficient and cost effective.

Principle of the ImmunoLight Assays for Immune Responses Using an Absorbance / Colorimetric Readout
 
ImmunoLight™
  • Multiple
ImmunioLight™ TCP
  • Multiple, but not all assays available for all species. Please see online catalog.
ImmunoLight™ BCP
  • Human
For all assays:
  • Peripheral blood mononuclear cells (PBMC)
  • Umbilical cord blood
  • Bone marrow
  • Lymphocyte sub-populations
  • Lymphocyte tissues and organs (e.g. spleen, lymph nodes)

For Research Use Only. Not for clinical diagnostic use.

Absorbance plate reader or multimode plate reader with an absorbance filter at 490nm.

All ImmunoLight™ assay kits contain:
  • WST-1 Reagent
  • Sterile, clear 96-well plates
  • Sterile, adhesive foil covers
ImmunoLight™ TCP assay kits are available with the choice of:
  • IL-2 alone, or
  • CD3 + CD28 co-stimulators, or
  • IL-2 + CD3 + CD28 co-stimulators as a complete cocktail
ImmunoGlo™ BCP assay kits for human or mouse B-cells are available with the following cocktails:
  • Proprietary cocktail contains cytokines only.
  • Proprietary cocktail includes conjugates, ligands and cytokines (human)
  • Proprietary cocktail includes conjugates and cytokines (mouse).
ImmunoLight™ MLC contains the same contents for all ImmunoLight™ assays, but includes:
  • Mitomycin-C to perform a 1-way MLC

QuickGuide for ImmunoLight to Measure Immune Responses Using an Absorbance / Colorimetric Readout

QuickGuide to Perform ImmunoLight MLC (Mixed Lymphocyte Culture) Using an Absorbance / Colorimetric Readout
 

 

 

Download the ImmunoLight™ MLC Technical Manual

When ordering ImmunoLight™, simply click the Catalog Numberin the table below and you will be taken directly to the desired product.

ImmunoLight™ Assay Kits

Assay Type Catalog Number Species ImmunoGro™ Medium containing: Number of Plates/Samples
per Kit(#)
ImmunoLIGHT™ KM3-LPA-1 Multiple User defined 1 / 16-245

(#): Any number of replicate wells/sample can be used. However, Preferred Cell Systems™ recommends a minimum of 4 replicate wells/sample.
Human cell assays (H).  Primate cell assays (Pr). Rat cell assays (R). Mouse cell assays (M).

 

When ordering ImmunoLight™ TCP, simply click the Catalog Numberin the table below and you will be taken directly to the desired product.

ImmunoLight™ TCP Assay Kits

Assay Type Catalog Number Species ImmunoGro™ Medium containing: Number of Plates/Samples
per Kit(#)
ImmunoLight TCP™ KM3SF-TCP1-1H Human Serum-Free 1 / 16-245
ImmunoLight TCP™ KM3SF-TCP3-1H Human Serum-Free 1 / 16-245
ImmunoLight TCP™ KM3SF-TCP2-1H Human Serum-Free 1 / 16-245
ImmunoLight TCP™ KM3-TCP1-1Pr Primate Low serum 1 / 16-245
ImmunoLight TCP™ KM3-TCP1-1R Rat Low serum 1 / 16-245
ImmunoLight TCP™ KM3-TCP1-1M Mouse Low serum 1 / 16-245
ImmunoLight TCP™ KM3-TCP1-1H Human Low serum 1 / 16-245
ImmunoLight TCP™ KM3-TCP2-1H Human Low serum 1 / 16-245
ImmunoLight TCP™ KM3-TCP3-1H Human Low serum 1 / 16-245

(#): Any number of replicate wells/sample can be used. However, Preferred Cell Systems™ recommends a minimum of 4 replicate wells/sample.
Human cell assays (H).  Primate cell assays (Pr). Rat cell assays (R). Mouse cell assays (M).

 

When ordering ImmunoLight™ BCP, simply click the Catalog Numberin the table below and you will be taken directly to the desired product.

ImmunoLight™ Assay Kits
Assay Type Catalog Number Species ImmunoGro™ Medium containing: Number of Plates/Samples
per Kit(#)
ImmunoLight BCP™ KM3-BCP2-1H Human Low serum 1 / 16-245
ImmunoLight BCP™ KM3-BCP3-1H Human Low serum 1 / 16-245
ImmunoLight BCP™ KM3-BCP3-1M Mouse Low serum 1 / 16-245

(#): Any number of replicate wells/sample can be used. However, Preferred Cell Systems™ recommends a minimum of 4 replicate wells/sample.
Human cell assays (H).  Primate cell assays (Pr). Rat cell assays (R). Mouse cell assays (M).

 

When ordering ImmunoLight™ MLC, please copy the Catalog Number, click on Catalog Number Search and paste the number, followed by Search. You will be taken directly to the desired product.

 

ImmunoLight™ MLC Assay Kits

Assay Type Catalog Number Number of Plates
ImmunoLIGHT MLC™ KM3-MLC-1 1