MSCLight™

A Mesenchymal Stromal Cell (MSC) Viability and Proliferation Absorbance Assay, with Excellent Flow Cytometric Capability,
that Replaces CFU-F

Assays are also available Complete with Cryopreserved MSCs

 

 

MSCLight™

A Mesenchymal Cell (MSC) Fluorescence Viability and Proliferation Assay that Replaces the CFU-F Assay

Cell Population Tissue Formulation Catalog Number Quantity
Mesenchymal Stem/Stromal Cells Human Serum-free, Humanized KMCL-SF-1 1 Kit

MSCLight™ Complete

A Mesenchymal Cell (MSC) Absorbance Viability and Proliferation Assay Complete with Cryopreserved Cells

Cell Population Tissue Formulation Catalog Number Quantity
Mesenchymal Stem/Stromal Cells Complete with human bone marrow MSC Serum-free, Humanized KMCL-SF-BM1 1 Kit
Mesenchymal Stem/Stromal Cells Complete with human cord blood MSC Serum-free, Humanized KMCL-SF-CB1 1 Kit

 

MSCLight™: An absorbance research assay for mesenchymal stroll cells (MSC)

 

 

  • Determine the number of viable mesenchymal stem / stromal cells in proliferation.
  • Replaces the colony-forming unit - fibroblast (CFU-F) assay.
  • Replaces all MTT assays.
  • Experimental transplantation and regeneration models.
  • Effects of growth factors/cytokines.
  • In vivo to in vitro assays.
  • Gene targeting assays.
  • Uses an ELISA plate reader, available in most laboratories.
  • No CFU-F colony counting needed.
  • Non-subjective, instrument-based and quantitative.
  • Kits include high performance MSCGro™ medium with either low serum or serum free formulations.
  • MSCLight™ can be used with fluorescence-labeled and GLP-labeled mesenchymal stem cells from Preferred Cell Systems™.
  • Combine/multiplex MSCLight™ with flow cytometry by labeling the same cells in culture after performing MSCLight™.
  • Results in about 3-5 days depending on species and origin of MSC.
  • Complete assay kit includes everything needed to culture and measure mesenchymal stem cells from any tissue source.
  • Simple, time efficient and cost effective.

MSCLight™ Complete

Includes a vial of frozen MSCs derived from human umbilical cord blood or bone marrow. 



 
  • Human
  • Non-human primate
  • Rat
  • Mouse
  • Dog
  • Horse
  • Low serum, complete
  • Serum-free, xenon-free complete
  • Humanized, serum-free, complete

For Research Use Only

An absorbance or multimode plate reader to measure absorbance at 490nm.

  • MSCGro™ Medium formulation of choice
  • Sterile, 96-well plate(s)
  • Non-sterile, 96-well plate(s)
  • Sterile, adhesive cover foils to maintain sterility of unused wells
 

Kit Contents for MSCLight™ Complete

  • 1 Vial of cryopreserved human umbilical cord blood or bone marrow MSCs
  • MSCGro™ Medium formulation of choice
  • Sterile, 96-well plate(s)
  • Non-sterile, 96-well plate(s)
  • Sterile, adhesive cover foils to maintain sterility of unused wells

MSCLight™ Assay Manual

Absorbance mesenchymal stem cell (MSC) proliferation assay.