Description	Catalog Number	Quantity
An Add-On Kit for Any Bioluminescence "GLO" Assay	K-ATPSC-1	1 Kit

MSCGlo™

A Mesenchymal Cell (MSC) Bioluminescence Viability and Proliferation Assay that Replaces the CFU-F Assay

Cell Population	Tissue	Formulation	Catalog Number	Quantity
Mesenchymal Stem/Stromal Cells	Human	Serum-free, Humanized	KLMC-SF-1	1 Kit

MSCGlo™ Complete

A Mesenchymal Cell (MSC) Bioluminescence Viability and Proliferation Assay Complete with Cryopreserved Cells

Cell Population	Tissue	Formulation	Catalog Number	Quantity
Mesenchymal Stem/Stromal Cells	Complete with human bone marrow MSC	Serum-free, Humanized	KLMC-SF-BM1	1 Kit
Mesenchymal Stem/Stromal Cells	Complete with human cord blood MSC	Serum-free, Humanized	KLMC-SF-CB1	1 Kit

• All research applications involving mesenchymal stem/progenitor cells and cells derived from MSCs.
• Replacement for the colony-forming unit - fibroblast (CFU-F) assay.
• Stromal cell assay applications.
• Combination of MSC proliferation with other readouts, e.g. flow cytometry, gene expression.
• Immunoregulation involving MSC/MPC.
• Self-renewal and differentiation capability applications.
• Clinical and regenerative medicine applications.
• Determine the effects of growth factors/cytokines on MSCs and their differentiation.
• MSC expansion.
• In vivo to in vitro assays.
• MSC gene targeting and expression, e.g. CRISPR-Cas9.

• Uses the chemical energy of intracellular ATP to measure MSC viability, proliferation ability, potential and differentiation.
• Replaces the CFU-F assay with a more reliable, robust, non-subjective, instrument-based assay.
• Allows population doubling time (PDL), viability, proliferation ability and proliferation potential to be measured in a single assay.
• Choose between low serum, serum-free/xeno-free or humanized media included with the assays. Human platelet lysate can also be used.
• Monitor small, medium and large (bulk) MSC/MPC expansion (passaging).
• Assay kits can be customized to include different formulations of MSCGro™ Medium.
• Optional ATP Bioluminescence Standardization & Calibration Kit (Cat. No. K-ATPSC-1) available (see below).
• Multiplex with flow cytometry for phenotypic analysis or gene expression analysis to obtain and correlate information from a single sample.

MSCGlo™-96 Complete

Includes a vial of frozen MSCs derived from either human umbilical cord blood or bone marrow.

By using the ATP Bioluminescence Standardization & Calibration Kit (Cat. No. K-ATPSC-1), non-standardized RLU values can be converted to standardized ATP concentrations. Scroll down to download the manual for this kit. Controls are used to calibrate the luminescence plate reader and the ATP standard is used to standardize the assay. The values obtained from the standard curve and controls (see diagram below) are then compared to the measurement assurance parameters that are provided in the instruction manual. If the values obtained are within the ranges provided by the measurement assurance parameters, you, the user, have successfully performed the proficiency testing to ensure that you have not only performed the assay correctly, but that the results you obtain can be trusted.

No additional proficiency testing is required if the calibration and standardization procedure is performed. The values you obtain from each calibration and standardization can be logged and used for certification that the assay has been performed correctly and that the results are trustworthy.


 

• Human
• Non-human primate
• Rat
• Mouse
• Dog
• Horse

MSCGro™ Media Available

• Low serum, complete
• Serum-free, xeno-free, complete
• Humanized, serum-free, complete (for human cells only)

 

Mesenchymal Stem/Stromal Cells Available (all human)

• Bone marrow
• Umbilical cord blood-derived MSCs

For Research Use Only. Not for clinical diagnostic use.
Luminescence or multimode plate reader with "glow" luminescence measuring capability.

• MSCGro™ Medium
• ATP Enumeration Reagent
• Sterile, 96-well plates
• Sterile, adhesive foil covers

PLEASE NOTE: If required, MSCGlo™ and MSCGlo™ Complete can be standardized using the add-on ATP Bioluminescence Standardization and Calibration Kit, Catalog Number K-ATPSC-1.

Kit Contents for MSCGlo™ Complete

• 1 Vial of cryopreserved human umbilical cord blood or bone marrow MSCs
• MSCGro™ Medium
• ATP Enumeration Reagent
• Sterile, 96-well plates
• Sterile, adhesive foil covers

 

Download the MSCGlo™ Manual
Download the ATP Bioluminescence Standardization & Calibration Kit Manual
Download SDS for ATP Enumeration Reagent
Download SDS for ATP Standards and Controls Download Certificate of Analysis (CoAs) for ATP Enumeration Reagent (ATP-ER)
Download Certificate of Analysis for ATP Stanadrds
Download Certificate of Analysis for ATP Controls
Download Certificate of Analysis of ATP Reconstitution Reagent
Download Certificate of Analysis for Sterile 96-Well Plates
Download Certificate of Analysis for Non-Sterile, 96-Well Plates
Download Certificate of Analysis for Sealing Films
Download Certificate of Analysis for IMDM