MSCFluor™

A Mesenchymal Stromal Cell (MSC) Viability and Proliferation Fluorescence Assay, with Excellent Flow Cytometric Capability
that Replaces CFU-F

Assays are also available Complete with Cryopreserved MSCs
 
 

 

MSCFluor™

A Mesenchymal Cell (MSC) Fluorescence Viability and Proliferation Assay that Replaces the CFU-F Assay

Cell Population Tissue Formulation Catalog Number Quantity
Mesenchymal Stem/Stromal Cells Any Low serum KMCF-LS-1 1 Kit
Mesenchymal Stem/Stromal Cells Human Serum-free, Humanized KMCF-SF-1 1 Kit

MSCFluor™ Complete

A Mesenchymal Cell (MSC) Fluorescence Viability and Proliferation Assay Complete with Cryopreserved Cells

Cell Population Tissue Formulation Catalog Number Quantity
Mesenchymal Stem/Stromal Cells Complete with human bone marrow MSC Low serum KMCF-LS-BM1 1 Kit
Mesenchymal Stem/Stromal Cells Complete with human bone marrow MSC Serum-free, Humanized KMCF-SF-BM1 1 Kit
Mesenchymal Stem/Stromal Cells Complete with human cord blood MSC Low serum KMCF-LS-CB1 1 Kit
Mesenchymal Stem/Stromal Cells Complete with human cord blood MSC Serum-free, Humanized KMCF-SF-CB1 1 Kit

 

MSCFluor™: A fluorescence research assay for mesenchymal stroll cells (MSC) 
 
 
  • Measures viability and proliferation of Mesenchymal Stromal Cells (MSC).
  • Compare MSC characteristics of naive and passaged MSCs over time.
  • Combine directly with phenotypic analysis by flow cytometry.
  • Replaces the colony-forming unit - fibroblast (CFU-F) assay.
  • Experimental transplantation and regeneration models.
  • Effects of growth factors/cytokines.
  • In vivo to in vitro assay.
  • Gene targeting assays.
  • Replaces the CFU-F assay.
  • Non-subjective, instrument-based and quantitative.
  • Includes high performance MSCGro™ Medium.
  • Multiplexes with bioluminescence assays.
  • Combine or multiplex directly with flow cytometry by labeling the same cells in culture after performing MSCFluor™.
  • Results in about 3-5 days depending on species and origin of MSC.
  • Allows the cells to be detected and characterized by flow cytometry using other excitation and emission wavelengths.
  • Simple, Time efficient and cost effective.

MSCFluor™ Complete

Includes a vial of cryopreserved human MSCs derived from either umbilical cord blood or bone marrow.

  • Human
  • Non-human primate
  • Rat
  • Mouse
  • Dog 
  • Horse

Principle of Measuring Mesenchymal Stem Cell Proliferation using a Resazurin (alamarBlue™) Readout
 
  • Low serum, complete
  • Serum-free, xeno-free complete
  • Humanized, serum-free complete

For Research Use Only.

A fluorescence plate reader or multiparameter plate reader with an excitation filter at 560nm and an emission filter at 590nm.

  • MSCGro™ Medium of choice
  • Resazurin Reagent
  • 3% SDS to stop reaction
  • Resorufin to standardize the assay
  • Non-sterile 96-well plate to perform assay standardization
  • Sterile, 96-well plate(s)
  • Sterile, adhesive cover foils to maintain sterility of unused wells
 

Kit Contents of MSCFluor™-96 Complete

  • Same as above, but including,
  • 1 vial of cryopreserved human umbilical cord blood or bone marrow MSCs

QuickGuide for MSCFluor™ to Measure MSC Viability and Proliferation