MSCFluor™
A Mesenchymal Stromal Cell (MSC) Viability and Proliferation Fluorescence Assay, with Excellent Flow Cytometric Capability
that Replaces CFU-F
Assays are also available Complete with Cryopreserved MSCs
Buy MSCFluor™
MSCFluor™
A Mesenchymal Cell (MSC) Fluorescence Viability and Proliferation Assay that Replaces the CFU-F Assay
| Cell Population | Tissue | Formulation | Catalog Number | Quantity |
|---|---|---|---|---|
| Mesenchymal Stem/Stromal Cells | Human | Serum-free, Humanized | KMCF-SF-1 | 1 Kit |
MSCFluor™ Complete
A Mesenchymal Cell (MSC) Fluorescence Viability and Proliferation Assay Complete with Cryopreserved Cells
| Cell Population | Tissue | Formulation | Catalog Number | Quantity |
|---|---|---|---|---|
| Mesenchymal Stem/Stromal Cells | Complete with human bone marrow MSC | Serum-free, Humanized | KMCF-SF-BM1 | 1 Kit |
| Mesenchymal Stem/Stromal Cells | Complete with human cord blood MSC | Serum-free, Humanized | KMCF-SF-CB1 | 1 Kit |
Uses
- Measures viability and proliferation of Mesenchymal Stromal Cells (MSC).
- Compare MSC characteristics of naive and passaged MSCs over time.
- Combine directly with phenotypic analysis by flow cytometry.
- Replaces the colony-forming unit - fibroblast (CFU-F) assay.
- Experimental transplantation and regeneration models.
- Effects of growth factors/cytokines.
- In vivo to in vitro assay.
- Gene targeting assays.
- Replaces the CFU-F assay.
- Non-subjective, instrument-based and quantitative.
- Includes high performance MSCGroâ„¢ Medium.
- Multiplexes with bioluminescence assays.
- Combine or multiplex directly with flow cytometry by labeling the same cells in culture after performing MSCFluorâ„¢.
- Results in about 3-5 days depending on species and origin of MSC.
- Allows the cells to be detected and characterized by flow cytometry using other excitation and emission wavelengths.
- Simple, Time efficient and cost effective.
MSCFluor™ Complete
Includes a vial of cryopreserved human MSCs derived from either umbilical cord blood or bone marrow.
- Human
- Non-human primate
- Rat
- Mouse
- Dog
- Horse
- Low serum, complete
- Serum-free, xeno-free complete
- Humanized, serum-free complete
For Research Use Only.
A fluorescence plate reader or multiparameter plate reader with an excitation filter at 560nm and an emission filter at 590nm.
- MSCGroâ„¢ Medium of choice
- Resazurin Reagent
- 3% SDS to stop reaction
- Resorufin to standardize the assay
- Non-sterile 96-well plate to perform assay standardization
- Sterile, 96-well plate(s)
- Sterile, adhesive cover foils to maintain sterility of unused wells
Kit Contents of MSCFluorâ„¢-96 Complete
- Same as above, but including,
- 1 vial of cryopreserved human umbilical cord blood or bone marrow MSCs
