MSCGlo™ QC

Complete, Bioluminescence Assay to Optimize and Standardize Methods and Procedures to Ensure Mesenchymal Stem Cell Health for Regenerative Medicine

 

Buy MSCGlo™ QC Assay Kits

 

Complete, Bioluminescence Assay to Optimize and Standardize Methods and Procedures to Ensure Mesenchymal Stem Cell Health for Regenerative Medicine

 

  • For quantifying the "quality" of MSC/MPC in a stem cell product prior to use.
  • Determine whether a processing procedure affects MSC/MPC "quality" (proliferation ability).
  • Determine MSC/MPC "quality" pre- and post cryopreservation.
  • Determine MSC/MPC processing consistency and stability.
  • Directly compare MSC/MPC lots over time or manufactured under different conditions.
  • An ATP bioluminescence, instrument-based and quantitative assay to measure proliferation ability and cellular functionality of clinical-grade MSC/MPC for regenerative medicine.
  • Determines population doubling time (PDL), viability and proliferation ability (quality), in a single assay.
  • Customize to include either CRUXRUFA Human Platelet Lysate GMP or GMP Q grade or serum-free/xeno-free or humanized MSCGro™ Medium. 
  • Fully standardized, incorporating an external standard and controls for assay validation according to FDA regulations.
  • Assay can usually be completed in 5-7 days of culture depending on the batch of MSCs.
  • Easier, more time efficient and far more accurate than the traditional CFU-F assay.

Principle of ATP Bioluminescence Cell Proliferation Assays

All ATP bioluminescence assays kits from Preferred Cell Systems™ include an ATP standard(s) and controls.  Performing the ATP standard curve and controls is highly recommended. The controls are used to calibrate the luminescence plate reader and the ATP standard is used to standardize the assay. The values obtained from the standard curve and controls (see diagram below) are then compared to the measurement assurance parameters that are provided in the instruction manual. If the values obtained are within the ranges provided by the measurement assurance parameters, you, the user, have successfully performed the proficiency testing to ensure that you have not only performed the assay correctly, but that the results you obtain can be trusted.

No additional proficiency testing is required if the calibration and standardization procedure is performed. The values you obtain from each calibration and standardization can be logged and used for certification that the assay has been performed correctly and that the results are trustworthy.

Calibration and standardization of an ATP bioluminescence assay from Preferred Cell Systems™ - Measurement Assurance
 
  • CRUXRUFA Human Platelet Lysate
  • MSCGro™ Serum-free, xeno-free complete
  • MSCGro™ Humanized, complete

For Research Use Only. Not for clinical diagnostic use.

Luminescence or multimode plate reader with "glow" luminescence measuring capability.

  • CRUXRUFA™ Human Platelet Lysate OR
  • MSCGro™ Medium of choice
  • ATP standard
  • ATP controls
  • ATP Enumeration Reagent
  • Sterile, 96-well plates
  • Non-sterile, 96-well plates
  • Sterile, adhesive foil covers


Complete, Bioluminescence Assay to Optimize and Standardize Methods and Procedures to Ensure Mesenchymal Stem Cell Health for Regenerative Medicine

Download the MSCGlo™-QC Manual
Download the ATP Optimization Kit Protocol for First-Time Users
Download the Luminescence Plate Reader Setup and Conversion of RLU to ATP Values Protocol
Download the Certificates of Analysis (CoAs). Note: Some CoAs are included with the assay kits.
Download SDS for ATP Enumeration Reagent
Download SDS for ATP Standards and Controls

When ordering MSCGlo™ QC, simply click on the Catalog Number Search in the table below and you will be taken directly to the desired product.

Growth Medium Formulation Catalog Number Number of Plates/Kit Number of Samples/Kit*
Humanized KLMC-HMQC-1 1 8-24
Low serum KLMC-LSQC-1 1 8-24
Serum free KLMC-SFQC-1 1 8-24
CRUXRUFA™ Human Platelet Lysate KLMC-CRQC-1 1 8-24

* Depends on number of replicate wells prepared