HALO® Real Time

Visualization of Lympho-Hematopoietic Stem and Progenitor Cell Growth in Real Time

 

Buy HALO® Real Time Assay Kits

 

Bioluminescence real time hematopoietic stem and progenitor cell growth kinetic and viability assay.

  • Follow lympho-hematopoietic stem and progenitor cells in real time, as they grow in culture.
  • Detect cell viability and proliferation ability at any time in vitro.
  • Establish kinetic growth curves.
  • Measure cell doubling time.
  • Measure cell cycle parameters at different time points.
  • Determine and measure the presence of different cell populations with time by flow cytometric, phenotypic analysis.
  • Determine if frozen cell samples will grow, prior to using units of cells (e.g. cord blood).
  • Determine how different culture conditions, cell interactions, stimulators affect cell proliferation.
  • Determine the onset of cytotoxicity by drugs and xenobiotics.
  • HALO® Real Time is a non-destructive, non-lytic assay, allowing it to be combined (multiplexed) with virtually any other end-point assay.
  • Can be used as both a real time assay or as an end-point assay.
  • Can be combined with a standardized ATP bioluminescence assay such as HALO® or any other cellular therapy or toxicity assay.
  • Setup up in 96- or 384-well plates (included with the assay kits).
  • Reagent can be added at the start of culture or at any time after culture to follow the cells for up to 3-5 days.
  • Incorporates proprietary Suspension Expansion Culture™ (SEC™) Growth Medium to distinguish up to 7 different stem cell, and 9 different progenitor cell populations from 5 species. 
  • Simple, time efficient and cost effective.

Principle of Real Time Bioluminescence Cell Proliferation Assays
 
  • Human
  • Non-human primate (Macaca fascicularis (Cynomologus) and Macaca mulatta (Rhesus))
  • Dog
  • Rat
  • Mouse
See Lympho-Hematopoietic System to view cell populations and growth factor/cytokine cocktails).

The following proliferation status or potential can be detected for individual populations:


Stem Cells (SC)

SC-HPP 1: IL-3, IL-6, SCF, TPO, Flt3-L

SC-HPP 2: EPO, GM-CSF, IL-2, IL-3, Il-6, IL-7, SCF, TPO, Flt3-L

SC-GEMM 1: EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L

SC-GEMM 2: EPO, GM-CSF, IL-3, IL-6, SCF, TPO

SC-GEM 1: EPO, GM-CSF, IL-3, IL-6, SCF

SC-GEM 2: EPO, GM-CSF, IL-3, SCF

SC-GEM 3: EPO, GM-CSF, G-CSF, IL-3, SCF


Progenitor Cells (P)

P-BFU 1: EPO, IL-3, SCF

P-BFU 2: EPO

P-GM 1: GM-CSF, IL-3, SCF

P-GM 2: GM-CSF, G-CSF, IL-3, SCF

P-GM 3: GM-CSF

P-Mk 1: TPO, IL-3, SCF

P-Mk 2: TPO


Lymphopoietic Cells

P-Tcell: IL-2 (requires addition of co-stimulator)

P-Bcell: IL-7


No growth factors: Add growth factors/cytokines of choice.

When ordering HALO® Real Time, please use the Catalog Number and Species. You can quickly find these products by copying the catalog number and entering it in the advanced search.
Advanced Search

LOW SERUM ASSAY KITS

Cell Population Human Primate Dog Rat Mouse
SC-HPP 1 K6-HPP1-1H K6-HPP1-1Pr K6-HPP1-1C K6-HPP1-1R K6-HPP1-1M
SC-HPP 2 K6-HPP2-1H K6-HPP2-1Pr K6-HPP2-1C K6-HPP2-1R K6-HPP2-1M
SC-GEMM 1 K6-GEMM1-1H K6-GEMM1-1Pr K6-GEMM1-1C K6-GEMM1-1R K6-GEMM1-1M
SC-GEMM 2 K6-GEMM2-1H K6-GEMM2-1Pr K6-GEMM2-1C K6-GEMM2-1R K6-GEMM2-1M
SC-GEM 1 K6-GEM1-1H K6-GEM1-1Pr K6-GEM1-1C K6-GEM1-1R K6-GEM1-1M
SC-GEM 2 K6-GEM2-1H K6-GEM2-1Pr K6-GEM2-1C K6-GEM2-1R K6-GEM2-1M
P-BFU 1 K6-BFU1-1H K6-BFU1-1Pr K6-BFU1-1C K6-BFU1-1R K6-BFU1-1M
P-BFU 2 K6-BFU2-1H K6-BFU2-1Pr K6-BFU2-1C K6-BFU2-1R K6-BFU2-1M
P-GM 1 K6-GM1-1H K6-GM1-1Pr K6-GM1-1C K6-GM1-1R K6-GM1-1M
P-GM 3 K6-GM3-1H K6-GM3-1Pr K6-GM3-1C K6-GM3-1R K6-GM3-1M
P-MK 1 K6-Mk1-1H K6-Mk1-1Pr K6-Mk1-1C K6-Mk1-1R K6-Mk1-1M

 

 

SERUM-FREE ASSAY KITS

Cell Population Human Primate Mouse
SC-HPP 1 K6SF-HPP1-1H K6SF-HPP1-1Pr K6SF-HPP1-1M
SC-HPP 2 K6SF-HPP2-1H K6SF-HPP2-1Pr K6SF-HPP2-1M
SC-GEMM 1 K6SF-GEMM1-1H K6SF-GEMM1-1Pr K6SF-GEMM1-1M
SC-GEMM 2 K6SF-GEMM2-1H K6SF-GEMM2-1Pr K6SF-GEMM2-1M
SC-GEM 1 K6SF-GEM1-1H K6SF-GEM1-1Pr K6SF-GEM1-1M
SC-GEM 2 K6SF-GEM2-1H K6SF-GEM2-1Pr K6SF-GEM2-1M
P-BFU 1 K6SF-BFU1-1H K6SF-BFU1-1Pr K6SF-BFU1-1M
P-BFU 2 K6SF-BFU2-1H K6SF-BFU2-1Pr K6SF-BFU2-1M
P-GM 1 K6SF-GM1-1H K6SF-GM1-1Pr K6SF-GM1-1M
P-GM 3 K6SF-GM3-1H K6SF-GM3-1Pr K6SF-GM3-1M
P-MK 1 K6SF-Mk1-1H K6SF-Mk1-1Pr K6SF-Mk1-1M
For Research Use Only. Not for clinical diagnostic use.

Luminescence plate reader or multimode plate reader with "glow" luminescence measuring capability.
  • HALO® Master Mix
  • HALO®-Real Time Reagent
  • Sterile, 96-well plates
  • Sterile, adhesive foil covers

HALO® Real Time Assay Manual

Bioluminescence real time hematopoietic stem and progenitor cell growth kinetic and viability assay.