HALO® Real Time
Bioluminescence Real Time Hematopoietic Stem and Progenitor Cell Growth Kinetic and Viability AssayUses
- Follow lympho-hematopoietic stem and progenitor cells in real time, as they grow in culture.
- Detect cell viability and proliferation ability at any time in vitro.
- Establish kinetic growth curves.
- Measure cell doubling time.
- Measure cell cycle parameters at different time points.
- Determine and measure the presence of different cell populations with time by flow cytometric, phenotypic analysis.
- Determine if frozen cell samples will grow, prior to using units of cells (e.g. cord blood).
- Determine how different culture conditions, cell interactions, stimulators affect cell proliferation.
- Determine the onset of cytotoxicity by drugs and xenobiotics.
- HALO® Real Time is a non-destructive, non-lytic assay, allowing it to be combined (multiplexed) with virtually any other end-point assay.
- Can be used as both a real time assay or as an end-point assay.
- Can be combined with a standardized ATP bioluminescence assay such as HALO® or any other cellular therapy or toxicity assay.
- Setup up in 96- or 384-well plates (included with the assay kits).
- Reagent can be added at the start of culture or at any time after culture to follow the cells for up to 3-5 days.
- Incorporates proprietary Suspension Expansion Culture™ (SEC™) Growth Medium to distinguish up to 7 different stem cell, and 9 different progenitor cell populations from 5 species.
- Simple, time efficient and cost effective.
- Non-human primate (Macaca fascicularis (Cynomologus) and Macaca mulatta (Rhesus))
The following proliferation status or potential can be detected for individual populations:
Stem Cells (SC)
• SC-HPP 1: IL-3, IL-6, SCF, TPO, Flt3-L
• SC-HPP 2: EPO, GM-CSF, IL-2, IL-3, Il-6, IL-7, SCF, TPO, Flt3-L
• SC-GEMM 1: EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L
• SC-GEMM 2: EPO, GM-CSF, IL-3, IL-6, SCF, TPO
• SC-GEM 1: EPO, GM-CSF, IL-3, IL-6, SCF
• SC-GEM 2: EPO, GM-CSF, IL-3, SCF
• SC-GEM 3: EPO, GM-CSF, G-CSF, IL-3, SCF
Progenitor Cells (P)
• P-BFU 1: EPO, IL-3, SCF
• P-BFU 2: EPO
• P-GM 1: GM-CSF, IL-3, SCF
• P-GM 2: GM-CSF, G-CSF, IL-3, SCF
• P-GM 3: GM-CSF
• P-Mk 1: TPO, IL-3, SCF
• P-Mk 2: TPO
• P-Tcell: IL-2 (requires addition of co-stimulator)
• P-Bcell: IL-7
No growth factors: Add growth factors/cytokines of choice.
Luminescence plate reader or multimode plate reader with "glow" luminescence measuring capability.
- HALO® Master Mix
- HALO®-Real Time Reagent
- Sterile, 96-well plates
- Sterile, adhesive foil covers