ImmunoGlo™ Real Time

Visualization of Immune Cell Growth in Real Time


Buy ImmunoGlo™ Real Time Assay Kits
 

Real time cell proliferation kinetics and viability of lymphopoietic and immune cells
 

  • Follow lymphocyte proliferation in real time.
  • Study lymphocyte growth kinetics under different conditions.
  • Estimate doubling time under different conditions for lymphocyte proliferation.
  • Non-radioactive, non-destructive assay incorporating bioluminescence technology.
  • Instrument-based, quantitative.
  • Add ImmunoGlo™ Real Time reagent at the beginning of culture or at any time after culture initiation, and follow cell growth for up to 3 days.
  • Cell proliferation can be measured repeatedly at any time.
  • More sensitive than WST-1, CSFE and other absorbance or fluorescence assays.
  • Complete flexibility to use any mitogens, co-stimulators etc and lymphocyte culture protocols.
  • Combine directly with flow cytometry to determine the phenotype of cells directly in the well at any time during culture and monitor the change in phenotype.

Principle of Real Time Bioluminescence Cell Proliferation/Cytotoxicity Assays

  • Human (only for ImmunoGlo TCP)
  • Non-human primate
  • Dog
  • Rat
  • Mouse
  • Peripheral blood mononuclear cells (PBMC)
  • Lymphocyte sub-populations
  • Lymphocyte tissues and organs

When ordering ImmunoGlo™ Real Time, please use the Catalog Number. You can quickly find this product by copying the catalog number and pasting it in the advanced search.
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Assay Type Catalog Number Number of Plates/Kit Number of Samples/Kit(#)
ImmunoGlo™ Real Time KM1-RT-1 1 16 - 24


 (#): Preferred Cell Systems™ recommends a minimum of 4 replicate wells/sample, particularly if flow cytometry is to be performed.

For Research Use Only. Not for clinical diagnostic use.

Luminescence or multimode plate reader with "glow" luminescence measuring capability.

  • ImmunoGro™ Medium
  • Real Time reagent buffer
  • Real Time bioluminescence reagent
  • Sterile 96-well plate
  • Sterile, adhesive plate cover foils to maintain sterility of unused wells

ImmunoGlo™ Real Time Assay Manual

A bioluminescence assay to follow the growth kinetics of different immune cells in culture.