ImmunoFluor™

An In Vitro Fluorescence Basic Viability and Proliferation Assay for Immune Cells

Cell Population	Species/Tissue	ImmunoGro™ Medium Formulation	Catalog Number	Quantity
Immune cells	Multiple / Any	ImmunoGro™ Base Medium	KM2-LPA-1	1 Kit

ImmunoFluor™ TCP

An In Vitro Fluorescence T-Lymphocyte Proliferation Assay

Cell Population	Species/Tissue	ImmunoGro™ Medium Formulation	Catalog Number	Quantity
T-lymphocytes	Human / Any	Low Serum + IL-2	KM2-TCP1-1H	1 Kit
T-lymphocytes	Human / Any	Low Serum + CD3 + CD28	KM2-TCP2-1H	1 Kit
T-lymphocytes	Human / Any	Low Serum + IL-2 + CD3 + CD28	KM2-TCP3-1H	1 Kit
T-lymphocytes	Human / Any	Serum-free + IL-2	KM2SF-TCP1-1H	1 Kit
T-lymphocytes	Human / Any	Serum-free + CD3 + CD28	KM2SF-TCP2-1H	1 Kit
T-lymphocytes	Human / Any	Serum-free + IL-2 + CD3 + CD28	KM2SF-TCP3-1H	1 Kit

ImmunoFluor™ TCP

An In Vitro Fluorescence T-Lymphocyte Proliferation Assay

Cell Population	Species/Tissue	ImmunoGro™ Medium Formulation	Catalog Number	Quantity
T-lymphocytes	Primate / Any	Low Serum + IL-2	KM2-TCP1-1Pr	1 Kit

ImmunoFluor™ TCP

An In Vitro Fluorescence T-Lymphocyte Proliferation Assay

Cell Population	Species/Tissue	ImmunoGro™ Medium Formulation	Catalog Number	Quantity
T-lymphocytes	Rat / Any	Low Serum + IL-2	KM2-TCP1-1R	1 Kit

ImmunoFluor™ TCP

An In Vitro Fluorescence T-Lymphocyte Proliferation Assay

Cell Population	Species/Tissue	ImmunoGro™ Medium Formulation	Catalog Number	Quantity
T-lymphocytes	Mouse / Any	Low Serum + IL-2	KM2-TCP1-1M	1 Kit

ImmunoFluor™ BCP

An In Vitro Fluorescence B-Lymphocyte Proliferation Assay

Cell Population	Species/Tissue	ImmunoGro™ Medium Formulation	Catalog Number	Quantity
B-lymphocytes	Human / Any	Low Serum +Ligands+IL4	KM2-BCP2-1H
B-lymphocytes	Human / Any	Low Serum+Conjugates+Ligands+IL-4	KM2-BCP3-1H

ImmunoFluor™ BCP

An In Vitro Fluorescence B-Lymphocyte Proliferation Assay

Cell Population	Species/Tissue	ImmunoGro™ Medium Formulation	Catalog Number	Quantity
B-lymphocytes	Mouse / Any	Low Serum+Conjugates+IL-4	KM2-BCP3-1M	1 Kit

ImmunoFluor™ MLC

An In Vitro Fluorescence 1- or 2-Way Mixed Lymphocyte Culture (MLC) Assay

Cell Population	Species/Tissue	ImmunoGro™ Medium Formulation	Catalog Number	Quantity
Immune Cells	Multiple / Any	Includes Mitomycin-C for 1-way MLC	KM2-MLC-1	1 Kit

 

• ImmunoFluor™ is a general use functional immune cell proliferation assay where the investigator employs their own culture reagents and protocol for any type of immune cell.
• ImmunoFluor™ TCP Assay Kits include specific cocktails to stimulate functional T-lymphocytes.
• ImmunoFluor™ BCP is specific for stimulating functional human B-lymphocytes.
• ImmunoFluor™ MLC for a 1- or 2-way mixed lymphocyte culture (MLC) or reaction (MLR).

All assays incorporate reagents for a fluorescence readout. 
 

• Lymphocyte activation.
• T-lymphocyte proliferation.
• Test unprimed lymphocytes in the presence of antibodies, enterotoxins, mitogens etc.
• Cellular immune response studies.
• Single-cell, lymphocyte cloning studies.
• Test DLI (Donor Lymphocyte Infusion) samples for stimulation/induction prior to use.
• Effect of accessory on lymphocytes induction.
• Effect of co-stimulators on lymphocytes induction.
• Effect of epitope sequences and novel peptides or proteins.
• Test the response of primed T-cells in vitro.

Uses of ImmunoFluor™ MLC include:

• One-way or two-way mixed lymphocyte culture (MLC) / reaction (MLR).
• Allogeneic mixed lymphocyte culture.
• Dendritic cell interactions.
• Mesenchymal stem/stromal cell interactions.
• Agents that activate/suppress MLC/MLR.

• Non-subjective, instrument-based and quantitative.
• Easily multiplexes with flow cytometry protocols using other fluorescent labels, e.g. FITC, PE, PECy5, PerCP-Cy5, PE-Cy7 etc.
• Multiplexes with bioluminescence assays.
• Use in place of colorimetric/absorbance assays for greater sensitivity.
• Assay kit includes supplies for culture and cell processing.
• Simple, time efficient and cost effective. 

 

ImmunoFluor™ and ImmunoFluor™ TCP

• Human
• Non-human primate
• Mouse

ImmunoFluor™ BCP

• Human
• Mouse

• Peripheral blood mononuclear cells (PBMC)
• Lymphocyte sub-populations
• Lymphocyte tissues and organs

For Research Use Only. Not for clinical diagnostic use.

Fluorescence or multimode plate reader with an excitation filter of 560nm and emission filter of 590nm.

All ImmunoFluor™ Assay Kits contain:

• €‹Resazurin Reagent
• 3% SDS as stop reagent
• Resorufin to standardize the assay
• Non-sterile, 96-well plates for standardizing the assay
• Sterile, 96-well plates
• Sterile, adhesive foil covers

ImmunoFluor™ TCP assay kits are available with the choice of:

• IL-2 alone, or
• CD3 + CD28 co-stimulators, or
• IL-2 + CD3 + CD28 co-stimulators as a complete cocktail

ImmunoGlo™ BCP assay kits for human or mouse B-cells are available with the following cocktails:

• Proprietary cocktail contains cytokines only.
• Proprietary cocktail includes conjugates, ligands and cytokines (human)
• Proprietary cocktail includes conjugates and cytokines (mouse).

ImmunoFluor™ MLC contains the same contents for all ImmunoFluor™ assays, but includes:

• Mitomycin-C to perform a 1-way MLC